Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,414,784 G→T intergenic (+255204/+6153) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,414,7840GT95.5% 50.3 / ‑4.5 22intergenic (+255204/+6153)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (14/7);  minor base C (1/0);  total (15/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

ATTGTTTTGTTTGTTTAGATAAATTAGGTGGTGTGGTTGTCTTTTGCCAAGAAAAAATATCAATAAAGTTATCTTTACCTAGAGTATAATCTAAAATATTTTTCAGATTGATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGAGGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTAC  >  1/1414569‑1415020
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
aTTGTTTTGTTTGTTTAGATAAATTAGGTGGTGTGGTTGTCTTTTGCCAAGAAAAAATATCAATAAAGTTATCTTTACCTAGAGTATAATCTAAAATATTTTTCAGATTGATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGAc                                                                                                                                                                                                                           >  1:602467/1‑235 (MQ=255)
                        tAGGTGGTGTGGTTGTCTTTTGCCAAGAAAAAATATCAATAAAGTTATCTTTACCTAGAGTATAATCTAAAATATTTTTCAGATTGATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATAcggc                                                                                                                                                                                   <  1:798706/251‑1 (MQ=255)
                              gtgtggtTGTCTTTTGCCAAGAAAAAATATCAATAAAGTTATCTTTACCTAGAGTATAATCTAAAATATTTTTCAGATTGATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGACGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCa                                                                                                                                                                             >  1:570972/1‑251 (MQ=255)
                                                                                   gTATAATCTAAAATATTTTTCAGATTGATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATg                                                                                                                         >  1:596201/1‑250 (MQ=255)
                                                                                                          aTTGATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTc                                                                                                                                                                                                >  1:203400/1‑156 (MQ=255)
                                                                                                             gATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATAcggcgg                                                                                                                                                                                 >  1:514880/1‑168 (MQ=255)
                                                                                                                 aTCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTc                                                                                                                                                                                                >  1:16440/1‑149 (MQ=255)
                                                                                                                  tctcATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATc                                                                                                                                              >  1:887312/1‑198 (MQ=255)
                                                                                                                     cATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATc                                                                                                                                              >  1:8893/1‑195 (MQ=255)
                                                                                                                           catcaATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATc                                                                                                                                              <  1:256955/189‑1 (MQ=255)
                                                                                                                           catcaATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTaa                                                                                                                           <  1:182174/208‑1 (MQ=255)
                                                                                                                                                   cATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCa                                                                                                                                                                                                                                      >  1:516599/1‑77 (MQ=255)
                                                                                                                                                     tCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTc                                                                                                                                                                                                <  1:486267/113‑1 (MQ=255)
                                                                                                                                                                 aTGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATAc                                                                                                                                                                   <  1:434224/130‑1 (MQ=255)
                                                                                                                                                                              cTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATc                                                                                                                                              >  1:741165/1‑138 (MQ=255)
                                                                                                                                                                                           atataAAAAAGATGCCCATGTAGAATGATGATAGCCATCTTTATAg                                                                                                                                                                                                                             <  1:324100/46‑1 (MQ=255)
                                                                                                                                                                                                  aaaGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAAtata                                                                                                                                                                        >  1:852979/1‑92 (MQ=255)
                                                                                                                                                                                                  aaaGATGCCCATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATccacca          >  1:404538/1‑250 (MQ=255)
                                                                                                                                                                                                         cccATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGtgc  <  1:129838/251‑3 (MQ=255)
                                                                                                                                                                                                           cATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATAc                                                                                                                                                                   >  1:368111/1‑88 (MQ=255)
                                                                                                                                                                                                           cATGTAGAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATtaata                                                                                              >  1:40407/1‑157 (MQ=255)
                                                                                                                                                                                                                 gAATGATGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTatat                                                                                                                                               >  1:361707/1‑102 (MQ=255)
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
ATTGTTTTGTTTGTTTAGATAAATTAGGTGGTGTGGTTGTCTTTTGCCAAGAAAAAATATCAATAAAGTTATCTTTACCTAGAGTATAATCTAAAATATTTTTCAGATTGATAATCTCATTATCATCAATACTTGATAAAAAAATTGCATCTTTATTCAAAATGCTCTTGGAGGCTTTTGATAAATTATATAAAAAAGATGCCCATGTAGAATGAGGATAGCCATCTTTATAGACAAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTAC  >  1/1414569‑1415020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑