Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,413,663	A→G	intergenic (+254083/+7274)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,413,663	0	A	G	100.0%	193.5 / NA	71	intergenic (+254083/+7274)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/38);  total (33/38)

CTCATAATCCATGCGTTCCACATCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAACAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCTCCCGCTTCTGGCTTCCCCTCTTTCCATTTTTCGCTAAAAATCACTTTTTGTACGCGAGGT  >  1/1413424‑1413901                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               cTCATAATCCATGCGTTCCACATCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACcg                                                                                                                                                                                                                                      >  1:670661/1‑250 (MQ=255)       aTCCATGCGTTCCACATCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACttt                                                                                                                                                                                                                               >  1:411191/1‑251 (MQ=255)       aTCCATGCGTTCCACATCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCCCCGTCGAAAGCAACGAACCGCGACttt                                                                                                                                                                                                                               >  1:269378/1‑251 (MQ=255)                ttCCACATCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAACCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCaa                                                                                                                                                                                                                      >  1:317637/1‑251 (MQ=255)                ttCCACATCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATCTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCaa                                                                                                                                                                                                                      >  1:345744/1‑251 (MQ=255)                     caTCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTcc                                                                                                                                                                                                                             <  1:895338/239‑1 (MQ=255)                     caTCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTcc                                                                                                                                                                                                                             >  1:372711/1‑239 (MQ=255)                      aTCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCCACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCatat                                                                                                                                                                                                                <  1:441620/251‑1 (MQ=255)                        ctcGCCAAATAATTCAGGTTTTTTCCCCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:406056/251‑1 (MQ=255)                        ctcGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:111862/251‑1 (MQ=255)                        ctcGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:832257/251‑1 (MQ=255)                        ctcGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:849300/251‑1 (MQ=255)                        ctcGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:205033/251‑1 (MQ=255)                        ctcGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:77548/251‑1 (MQ=255)                              aaaTAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:225892/245‑1 (MQ=255)                                 taatTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAag                                                                                                                                                                                                     >  1:462598/1‑251 (MQ=255)                                                cACCGCTTGGAAGCGTACCCTCCGCCGACGCCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCAcc                                                                                                                                                                                      <  1:863663/251‑1 (MQ=255)                                                  ccGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:210254/225‑1 (MQ=255)                                                    gCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGtt                                                                                                                                                                                  >  1:25063/1‑251 (MQ=255)                                                          aaGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCatcaa                                                                                                                                                                            <  1:544044/251‑1 (MQ=255)                                                                       aCCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGtata                                                                                                                                                               >  1:808059/1‑251 (MQ=255)                                                                           acacCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCa                                                                                                                                                                                                                   >  1:289172/1‑195 (MQ=255)                                                                           acacCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGt                                                                                                                                                                                                             >  1:628702/1‑201 (MQ=255)                                                                           acacCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGt                                                                                                                                                            <  1:689120/250‑1 (MQ=255)                                                                           acacCATGCCACGCTCACGGCGATCATCTACCGTATTAACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGtt                                                                                                                                                           <  1:796123/251‑1 (MQ=255)                                                                               cATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTgg                                                                                                                                                         >  1:755621/1‑249 (MQ=255)                                                                                aTGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAag                                                                                                                                                                                                     <  1:19551/204‑1 (MQ=255)                                                                                  gCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTtc                                                                                                                                                                                 >  1:164093/1‑222 (MQ=255)                                                                                      cGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTaa                                                                                                                                                >  1:827529/1‑251 (MQ=255)                                                                                         tCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTc                                                                                                                                             <  1:291088/251‑1 (MQ=255)                                                                                               cGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGAt                                                                                                                                        <  1:658569/250‑1 (MQ=255)                                                                                                 atcatcTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCGTTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGt                                                                                                                                     >  1:23582/1‑251 (MQ=255)                                                                                                 atcatcTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGt                                                                                                                                     >  1:520754/1‑251 (MQ=255)                                                                                                   catcTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGcaacaa                                                                                                                                                                                                      <  1:184326/184‑1 (MQ=255)                                                                                                      cTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAg                                                                                                                                >  1:712619/1‑251 (MQ=255)                                                                                                        aCCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGt                                                                                                                              <  1:768629/251‑1 (MQ=255)                                                                                                        aCCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGt                                                                                                                              <  1:674559/251‑1 (MQ=255)                                                                                                        aCCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGt                                                                                                                              <  1:411852/251‑1 (MQ=255)                                                                                                           gTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCatcaa                                                                                                                                                                            <  1:229727/202‑1 (MQ=255)                                                                                                           gTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTAt                                                                                                                            >  1:537956/1‑250 (MQ=255)                                                                                                            tATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              <  1:15783/167‑1 (MQ=255)                                                                                                                cACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCAt                                                                                                                      <  1:881561/251‑1 (MQ=255)                                                                                                                  cccGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTg                                                                                                                                                                                                            <  1:258732/163‑1 (MQ=255)                                                                                                                  cccGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGAt                                                                                                                                        >  1:165165/1‑231 (MQ=255)                                                                                                                   ccGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGc                                                                                                                   <  1:615506/251‑1 (MQ=255)                                                                                                                             aTCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTc                                                                                                                                                                                                <  1:96176/164‑1 (MQ=255)                                                                                                                             aTCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTc                                                                                                                        <  1:467180/236‑1 (MQ=255)                                                                                                                                 aaTAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAac                                                                                                     <  1:556860/251‑1 (MQ=255)                                                                                                                                   tAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAacac                                                                                                   <  1:221428/251‑1 (MQ=255)                                                                                                                                    aGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAaca                                                                                                    >  1:179404/1‑249 (MQ=255)                                                                                                                                     gTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGAc                                                                                                                                                                                                                                  >  1:401716/1‑122 (MQ=255)                                                                                                                                     gTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGt                                                                                                                              >  1:208209/1‑222 (MQ=255)                                                                                                                                         aaGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTcac                                                                                             <  1:8859/251‑1 (MQ=255)                                                                                                                                           ggTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGc                                                                                                                   <  1:889676/227‑1 (MQ=255)                                                                                                                                           ggTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACaa                                                                                           >  1:287870/1‑251 (MQ=255)                                                                                                                                                     aaaTCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCAcc                                                                                                                                                                                      >  1:10034/1‑150 (MQ=255)                                                                                                                                                                         aTACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGc                                                                               <  1:483389/233‑1 (MQ=255)                                                                                                                                                                            cGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTg                                                                                                                                                                                    <  1:538041/129‑1 (MQ=255)                                                                                                                                                                              ccccTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTc                                                                                                               <  1:252345/196‑1 (MQ=255)                                                                                                                                                                                   gCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGt                                                                                                                                                  <  1:409781/156‑1 (MQ=255)                                                                                                                                                                                               gCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCg                                                                                     <  1:545782/205‑1 (MQ=255)                                                                                                                                                                                                cAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCttt                                       <  1:98789/250‑1 (MQ=255)                                                                                                                                                                                                   tatTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCTTTCCAt                                   >  1:757145/1‑251 (MQ=255)                                                                                                                                                                                                    atTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCTTTCCAtt                                  >  1:264883/1‑251 (MQ=255)                                                                                                                                                                                                    atTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCTTTCCAtt                                  >  1:475391/1‑251 (MQ=255)                                                                                                                                                                                                    atTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCTTTCCAtt                                  >  1:741860/1‑251 (MQ=255)                                                                                                                                                                                                                aTCGAACGGTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCTTTCCAtt                                  >  1:612168/1‑239 (MQ=255)                                                                                                                                                                                                                        gTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGc                                                                                                                   >  1:356652/1‑150 (MQ=255)                                                                                                                                                                                                                        gTTTGGCAAAATGCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCTTTCCATTTTTCGCTAAAAATCACtttt              >  1:224340/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCTCCGTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCCCCCGCTTCTGGCTTCCCCTCTTTCCATTTTTCGCTAAAAATCACTTTTTGTACGCGAGGt  >  1:809895/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTCGAAAGCAACGAACCGCGACTTTCCACATCAAGCATATAg                                                                                                                                                                                                              >  1:831145/1‑42 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               CTCATAATCCATGCGTTCCACATCTCGCCAAATAATTAAGGTTTTTTCACCGCTTGGAAGCGTACCCTCCACCGACACCATGCCACGCTCACGGCGATCATCTACCGTATTCACCCGCAACCCAATCAAATAGTTAAAGGTTTCCACTAAATCAATTTTTTGTGGCTGATACGCCCCTGCCGAGCTGGTGGCAATATTTAATTCGTAATCGAACGGTTTGGCAAAATGCTCCGTCGAAAACAACGAACCGCGACTTTCCACATCAAGCATATAGTGCAACAAGTAGTCATTTCGCACCTGTTCATCAAAATCAAAGGTATAAGTTGGCTTGCGTAATTCAAGATTGTTCAAGGTATCTTCATAGCTTTCCAACTTCAACACTTTCACAATTTGCGATACGCCATTCAAACCATTTTCTCCCGCTTCTGGCTTCCCCTCTTTCCATTTTTCGCTAAAAATCACTTTTTGTACGCGAGGT  >  1/1413424‑1413901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑