Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,406,930	G→A	intergenic (+247350/+14007)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,406,930	0	G	A	100.0%	199.6 / NA	73	intergenic (+247350/+14007)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/40);  total (33/40)

ATCCGCAAAAAACCCGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATCGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAGATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCTTTCCTTCCGCCCGTTAACGGAAATCATCCAA  >  1/1406693‑1407174                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     aTCCGCAAAAAACCCGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTAtt                                                                                                                                                                                                                                          <  1:514264/250‑1 (MQ=255)  tCCGCAAAAAACCCGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATtgg                                                                                                                                                                                                                                        <  1:572954/251‑1 (MQ=255)  accGCAAAAAACCCGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATGCAGTTATCCGCTTCTGCAAGGGCAGGATACCGTTGCCTTAGAAGCCGACGTTGAACGGGGCGGGACCGACCAAAAATTCAATTTATtgg                                                                                                                                                                                                                                        <  1:421369/250‑1 (MQ=255)           aaCCCGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTg                                                                                                                                                                                                                               <  1:551218/251‑1 (MQ=255)              ccGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGt                                                                                                                                                                                                                            <  1:687288/251‑1 (MQ=255)              ccGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGt                                                                                                                                                                                                                            <  1:850399/251‑1 (MQ=255)                 cATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTa                                                                                                                                                                                                                          >  1:581651/1‑250 (MQ=255)                     gtgtTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACaaaa                                                                                                                                                                                                                     >  1:105906/1‑251 (MQ=255)                                               aCTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTgg                                                                                                                                                                                           >  1:306405/1‑251 (MQ=255)                                                       ccGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTAcc                                                                                                                                                                                   <  1:669487/251‑1 (MQ=255)                                                       ccGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTAcc                                                                                                                                                                                   <  1:552641/251‑1 (MQ=255)                                                            ggCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGCGTTACAAAAATCCGCCGGTCAAAAACCATAAGTGGCAATTAccccgc                                                                                                                                                                               >  1:385260/1‑247 (MQ=255)                                                                        cTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTggg                                                                                                                                                                  <  1:814226/251‑1 (MQ=255)                                                                               gcaaTTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAg                                                                                                                                                                                              <  1:508328/216‑1 (MQ=255)                                                                                caaTTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACg                                                                                                                                                          >  1:586470/1‑251 (MQ=255)                                                                                caaTTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACg                                                                                                                                                          >  1:597765/1‑251 (MQ=255)                                                                                caaTTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACg                                                                                                                                                          >  1:80410/1‑251 (MQ=255)                                                                                        ccGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGt                                                                                                                                                                                                                                   >  1:636980/1‑170 (MQ=255)                                                                                             gCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAAt                                                                                                                                             >  1:724720/1‑251 (MQ=255)                                                                                              cccGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATg                                                                                                                                            >  1:763297/1‑251 (MQ=255)                                                                                                   aTGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGCCGGCGAGAAACAAATGTCCaa                                                                                                                                       >  1:31102/1‑251 (MQ=255)                                                                                                                   atTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTAc                                                                                                                       <  1:529145/251‑1 (MQ=255)                                                                                                                   atTTCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTAc                                                                                                                       <  1:280108/251‑1 (MQ=255)                                                                                                                     ttCAAAAAACGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACAt                                                                                                                     <  1:50078/251‑1 (MQ=255)                                                                                                                        aaaaaaCGCTTTTCAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCgg                                                                                                                  <  1:847647/251‑1 (MQ=255)                                                                                                                                     cAAATAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCa                                                                                                     >  1:866425/1‑251 (MQ=255)                                                                                                                                      aaaTAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCAc                                                                                                    <  1:796117/251‑1 (MQ=255)                                                                                                                                      aaaTAACCAACCGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCAc                                                                                                    <  1:388845/251‑1 (MQ=255)                                                                                                                                          aaccaaccGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAg                                                                                                >  1:653849/1‑251 (MQ=255)                                                                                                                                               accGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGaaa                                                                                           >  1:283770/1‑251 (MQ=255)                                                                                                                                               accGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGaaa                                                                                           >  1:282850/1‑251 (MQ=255)                                                                                                                                               accGATAGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGaaa                                                                                           >  1:430431/1‑251 (MQ=255)                                                                                                                                                      gCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCgg                                                                                    >  1:815189/1‑251 (MQ=255)                                                                                                                                                       cTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGt                                                                                                                                                                                                                            <  1:589632/114‑1 (MQ=255)                                                                                                                                                         aTTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTaa                                                                                 >  1:686093/1‑251 (MQ=255)                                                                                                                                                         aTTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTaa                                                                                 <  1:637361/251‑1 (MQ=255)                                                                                                                                                            cACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAAt                                                                              >  1:900800/1‑251 (MQ=255)                                                                                                                                                              cGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCa                                                                            <  1:370157/251‑1 (MQ=255)                                                                                                                                                                aaTTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTAt                                                                                                                                                                                                                                           >  1:279981/1‑90 (MQ=255)                                                                                                                                                                    cATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCa                                                                      >  1:772280/1‑251 (MQ=255)                                                                                                                                                                         aTCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTc                                                                  >  1:506417/1‑250 (MQ=255)                                                                                                                                                                              cTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATcc                                                                                                                                   <  1:686597/180‑1 (MQ=255)                                                                                                                                                                                   gCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTAccc                                                                                                                                                                                  <  1:104887/128‑1 (MQ=255)                                                                                                                                                                                     aaGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCGCAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAAtg                                                     <  1:90728/251‑1 (MQ=255)                                                                                                                                                                                     aaGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAAtg                                                     <  1:321228/251‑1 (MQ=255)                                                                                                                                                                                     aaGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAAtg                                                     <  1:265965/251‑1 (MQ=255)                                                                                                                                                                                     aaGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAAtg                                                     <  1:545575/251‑1 (MQ=255)                                                                                                                                                                                        ggCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAAtgt                                                    <  1:381600/249‑1 (MQ=255)                                                                                                                                                                                          cAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTc                                                                                      >  1:315842/1‑213 (MQ=255)                                                                                                                                                                                             gATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGa                                                <  1:626586/248‑1 (MQ=255)                                                                                                                                                                                                tCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGta                                          >  1:475919/1‑251 (MQ=255)                                                                                                                                                                                                 ccGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGtat                                         <  1:82176/251‑1 (MQ=255)                                                                                                                                                                                                    ttGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAc                                      <  1:616222/251‑1 (MQ=255)                                                                                                                                                                                                     tGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAcc                                     <  1:738753/251‑1 (MQ=255)                                                                                                                                                                                                     tGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAcc                                     <  1:587759/251‑1 (MQ=255)                                                                                                                                                                                                     tGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAcc                                     <  1:535280/251‑1 (MQ=255)                                                                                                                                                                                                     tGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAcc                                     <  1:188573/251‑1 (MQ=255)                                                                                                                                                                                                      gCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCa                                                                                                                            <  1:210890/163‑1 (MQ=255)                                                                                                                                                                                                      gCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACgg                                                                                                          <  1:618420/181‑1 (MQ=255)                                                                                                                                                                                                      gCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAAtgt                                                    >  1:839724/1‑235 (MQ=255)                                                                                                                                                                                                      gCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAc                                      >  1:767423/1‑249 (MQ=255)                                                                                                                                                                                                      gCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAc                                      >  1:432977/1‑249 (MQ=255)                                                                                                                                                                                                       ccTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTcc                                                                                                                                         <  1:437839/149‑1 (MQ=255)                                                                                                                                                                                                        cTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAcc                                     >  1:96496/1‑248 (MQ=255)                                                                                                                                                                                                          tAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCtt                                <  1:570369/251‑1 (MQ=255)                                                                                                                                                                                                                   aCGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAAcc                                     <  1:515861/237‑1 (MQ=255)                                                                                                                                                                                                                   aCGTTGAACTTGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCTTTCCTTCCg                        >  1:813923/1‑250 (MQ=255)                                                                                                                                                                                                                             tGGCGGGACCGACCAAAAATTCAATTTATTTGTGGGGCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAAAGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGGATAAcc                                     >  1:851920/1‑227 (MQ=255)                                                                                                                                                                                                                             tGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCTTTCCTTCCGCCCGTTAACgg             <  1:21061/251‑1 (MQ=255)                                                                                                                                                                                                                             tGGCGGGACCGACCAAAAATTCAATTTATTGGTGGGGCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGGTTGGTATAACCTGCTTTCCTTCCGCCCGTTAACgg             >  1:356287/1‑251 (MQ=255)                                                                                                                                                                                                                                       gaccAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGATAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCTTTCCTTCCGcc                      >  1:442340/1‑232 (MQ=255)                                                                                                                                                                                                                                        accAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCTTTCCTTCCGCCCGTTAACGGAAATCATcc    <  1:842207/249‑1 (MQ=255)                                                                                                                                                                                                                                        accAAAAATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCTTTCCTTCCGCCCGTTAACGGAAATCATCCaa  >  1:383598/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     ATCCGCAAAAAACCCGCATCGTGTTTAACTCCGACTGGCTTGGTAAACTCGGCACCGAAGGCATGATTCGCCTGGCAAGCAATTACACCGTTGCCCGTATGCTGGAACGTGATGATTTCAAAAAACGCTTTTCAAATAACCAACCGATCGCTATTCACGAATTCATTTATCCGCTTCTGCAAGGGCAGGATTCCGTTGCCTTAGAAGCCGACGTTGAACTTGGCGGGACCGACCAAAGATTCAATTTATTGGTGGGTCGTGAGTTACAAAAATCCGCCGGTCAAAAACCACAAGTGGCAATTACCCTGCCGCTGTTGGTGGGCTTGGACGGCGAGAAAAAAATGTCCAAATCCCTTGGCAATTACATCGGCGTAACGGACGCACCGAGCGAAATGTTCGGTAAAATCATGTCCATTTCCGATGATCTAATGTGGGATTGGTATAACCTGCTTTCCTTCCGCCCGTTAACGGAAATCATCCAA  >  1/1406693‑1407174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑