Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,405,209	C→A	intergenic (+245629/+15728)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,405,209	0	C	A	100.0%	93.2 / NA	36	intergenic (+245629/+15728)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/21);  total (15/21)

GCATCATGTTCAACAATCACCTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTCAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTACCATCTCTCCAATCCGCAACCTTAATTC  >  1/1404975‑1405419                                                                                                                                                                                                                                           |                                                                                                                                                                                                                    gCATCATGTTCAACAATCACCTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGata                                                                                                                                                                                                     >  1:559817/1‑251 (MQ=255)           caacaaTCACCTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCt                                                                                                                                                                                           <  1:238296/251‑1 (MQ=255)                aTCACCTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTAc                                                                                                                                                                                      <  1:297998/251‑1 (MQ=255)                   aCCTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAAt                                                                                                                                                                                   <  1:166183/251‑1 (MQ=255)                    ccTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATa                                                                                                                                                                                  >  1:890597/1‑251 (MQ=255)                     cTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATAc                                                                                                                                                                                 <  1:399584/251‑1 (MQ=255)                     cTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCGATTACAATAc                                                                                                                                                                                 <  1:714360/251‑1 (MQ=255)                           gCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTaa                                                                                                                                                                                                <  1:803931/230‑1 (MQ=255)                                                                         cgACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACaa                                                                                                                                                                                    <  1:544176/196‑1 (MQ=255)                                                                           aCTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTAc                                                                                                                                                                                      >  1:632885/1‑192 (MQ=255)                                                                           aCTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCa                                                                                                                          >  1:394276/1‑251 (MQ=255)                                                                                                   aGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTAc                                                                                                                                                                                      <  1:602061/168‑1 (MQ=255)                                                                                                   aGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAAc                                                                                                   <  1:376541/250‑1 (MQ=255)                                                                                                     gTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACtt                                                                                                 <  1:679260/250‑1 (MQ=255)                                                                                                          ccAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGCAAAAAAAATTAGAAATTCATTACTAACTTTGCTATTTTAGCGAATTAATCGTTTTCAACAATTTAACTTTGgtt                                                                                            >  1:285428/1‑250 (MQ=255)                                                                                                          ccAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGgtg                                                                                            <  1:214151/250‑1 (MQ=255)                                                                                                            aaTTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAAc                                                                                                   <  1:137100/241‑1 (MQ=255)                                                                                                                 aCATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTAc                                                                                                                                                                                      >  1:831693/1‑154 (MQ=255)                                                                                                                 aCATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTAc                                                                                                                                                                                      >  1:830896/1‑154 (MQ=255)                                                                                                                             ccTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTaaaaaa                                                                        <  1:198066/251‑1 (MQ=255)                                                                                                                              cTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAAc                                                                                                   >  1:536341/1‑223 (MQ=255)                                                                                                                                       attAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCAc                                                              <  1:507234/251‑1 (MQ=255)                                                                                                                                                             ccccccAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAacac                                        <  1:156028/251‑1 (MQ=255)                                                                                                                                                             ccccccAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAacac                                        <  1:210791/251‑1 (MQ=255)                                                                                                                                                             ccccccAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATT‑CAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCg                                                                                                                   <  1:189082/175‑1 (MQ=255)                                                                                                                                                                 ccAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTaa                                                                                                                                                                                                >  1:847252/1‑96 (MQ=255)                                                                                                                                                                   aaGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTTACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTg                                                       >  1:501152/1‑230 (MQ=255)                                                                                                                                                                       gACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTa                              >  1:475159/1‑251 (MQ=255)                                                                                                                                                                            ttCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAAc                                                                                                                                           >  1:568006/1‑137 (MQ=255)                                                                                                                                                                                       ccAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTACCATCTCTCCAATc                <  1:667686/249‑1 (MQ=255)                                                                                                                                                                                       ccAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTACCATCTCTCCAATc                <  1:753976/249‑1 (MQ=255)                                                                                                                                                                                              gTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTACCATCTCTCCAATCCGCAACCtt       >  1:138613/1‑251 (MQ=255)                                                                                                                                                                                                aactaactCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAac                                                                                  >  1:130697/1‑174 (MQ=255)                                                                                                                                                                                                   taactCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTACCATCTCTCCAATCCGCAACCTTAATtc  <  1:880483/251‑1 (MQ=255)                                                                                                                                                                                                     actCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTAc                                                                                                                                                                                      <  1:308582/70‑1 (MQ=255)                                                                                                                                                                                                      ctctATCATTGATGAGGAAAAAGTAAAAAAATTTTTTAAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTACCATCTCTCCAATCCGCAACCTTAAtt   >  1:763769/1‑247 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                    GCATCATGTTCAACAATCACCTCAAAGCCAAGCTTCAAAATTTGCTGAACCGTCTTCGGCGTTGCCGCCACGCGACTTTCATTATCAAGTAGTTCTCTAGGTACACCAATTAACATAATGATTCCCTTTGATTGATTAATAATCCGGGTAAAATTACCCCCCAAGTGACTATTCTAAAACAGCCAAACTGTAACTAACTCTATCATTGATGAGGAAAAAGTAAAAAAATTTTTTCAGCTTTTTCTTTGATATGTAATCCCTATTACAATACTTCATTTATGC‑AAAAAAATTAGAAATTCACTACTAACTTTGCTATTTTAGCGCACTAATCGTTTTCCACTATTTAACTTTGGTGTGCTAAAAACACTTAAAAAATCAACCGCACTTTATTGTTCAAAGGTCAACACTATGCAATTACCATCTCTCCAATCCGCAACCTTAATTC  >  1/1404975‑1405419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑