Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,401,549	G→A	intergenic (+241969/+19388)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,401,549	0	G	A	100.0%	84.1 / NA	34	intergenic (+241969/+19388)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/13);  total (21/13)

GCTGTGGCATAGCTGTTTTTGCAATCGTTCCCATAAATAACCCGGATTGTCTGATTAATGACCTTTGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACGACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGTTTTTTCAATATCCGATTCTTTCCAATAGT  >  1/1401317‑1401786                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                gCTGTGGCATAGCTGTTTTTGCAATCGTTCCCATAAATAACCCGGATTGTCTGATTAATGACCTTTGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGaa                                                                                                                                                                                                                               >  1:798506/1‑251 (MQ=255)              tGTTTTTGCAATCGTTCCCATAAATAACCCGGATTGTCTGATTAATGACCTTTGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGc                                                                                                                                                                                                                  <  1:90477/251‑1 (MQ=255)                   ttGCAATCGTTCCCATAAATAACCCGGATTGTCTGATTAATGACCTTTGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATaac                                                                                                                                                                                                             <  1:304642/251‑1 (MQ=255)                             tCCCATAAATAACCCGGATTGTCTGATTAATGACCTTTGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGaa                                                                                                                                                                                                   <  1:657816/251‑1 (MQ=255)                              cccATAAATAACCCGGATTGTCTGATTAATGACCTTTGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGaaa                                                                                                                                                                                                  >  1:185794/1‑251 (MQ=255)                                                                 ttGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAAc                                                                                                                                                                                                                         >  1:229128/1‑193 (MQ=255)                                                                                     gTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGt                                                                                                                                          >  1:177594/1‑251 (MQ=255)                                                                                       tctcTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTAt                                                                                                                                        <  1:760479/251‑1 (MQ=255)                                                                                                                               ctctGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATCTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATc                                                                                               <  1:104212/251‑1 (MQ=255)                                                                                                                                 ctGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCa                                                                                             >  1:501393/1‑251 (MQ=255)                                                                                                                                                  aaaGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGcc                                                                            <  1:228904/251‑1 (MQ=255)                                                                                                                                                                aaTTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGat                                                              >  1:303982/1‑251 (MQ=255)                                                                                                                                                                        ttttAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGc                                                                             >  1:125148/1‑228 (MQ=255)                                                                                                                                                                             gTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGt                                                                                                     <  1:104093/199‑1 (MQ=255)                                                                                                                                                                               ataGATACGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAgg                                               <  1:449154/251‑1 (MQ=255)                                                                                                                                                                                  gATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAAc                                                   <  1:884205/244‑1 (MQ=255)                                                                                                                                                                                  gATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCaa                                            >  1:143188/1‑251 (MQ=255)                                                                                                                                                                                  gATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCaa                                            >  1:332834/1‑251 (MQ=255)                                                                                                                                                                                  gATATGTTTTTATGCGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGCGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCaa                                            >  1:42160/1‑251 (MQ=255)                                                                                                                                                                                       gTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTa                                                                                                                                                                                         <  1:500015/107‑1 (MQ=255)                                                                                                                                                                                       gTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTgg                                                        <  1:696604/234‑1 (MQ=255)                                                                                                                                                                                       gTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAAtt                                         >  1:64744/1‑249 (MQ=255)                                                                                                                                                                                       gTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAAtt                                         >  1:773100/1‑249 (MQ=255)                                                                                                                                                                                       gTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTT‑AAAAGGAAATAACTTTGAGGATAACCACACAAGAAACCTTTCGGAAGGAAGGCCAATTGCCCCCAAAAGTGACAACTTT‑AGAAGAAGGTGTATTTTTTACTAAAATTGACCAAT‑GCGAAATTAAAGTAAAATTCAATTATTAGGTCTTTACCAACCTTAAAAAGAATATTGGTTAAAAAGGCAAATTCtt                                      >  1:182205/1‑251 (MQ=255)                                                                                                                                                                                          tttATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGACTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTa                                    >  1:94508/1‑251 (MQ=255)                                                                                                                                                                                              tgtgTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCg                                <  1:633650/251‑1 (MQ=255)                                                                                                                                                                                                 gtTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAatga                                                                                         <  1:634533/191‑1 (MQ=255)                                                                                                                                                                                                 gtTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGttt                             >  1:550728/1‑251 (MQ=255)                                                                                                                                                                                                 gtTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGttt                             >  1:266396/1‑251 (MQ=255)                                                                                                                                                                                                         ccATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGt                                                                                                                                                                                          >  1:235863/1‑88 (MQ=255)                                                                                                                                                                                                         ccATTACTGTCGTTTTTCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAAGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGTTTTTTCAata                     >  1:850342/1‑251 (MQ=255)                                                                                                                                                                                                                       tttCAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGTTTTTTCAATATCCGATTc             >  1:564046/1‑245 (MQ=255)                                                                                                                                                                                                                          cAGTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGTTTTTTCAATATCCGATTCTTTCCAATa    >  1:414185/1‑251 (MQ=255)                                                                                                                                                                                                                            gTATGATGATGACAACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGTTTTTTCAATATCCGATTCTTTCCAATAGt  >  1:52911/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                GCTGTGGCATAGCTGTTTTTGCAATCGTTCCCATAAATAACCCGGATTGTCTGATTAATGACCTTTGTTAGTTTACTCATACTTGTTCTCTTAGCGCGGTAAAATTTTATTAGTGATTTGATTAATCTCTGATTTTATTTCGCATAAAGTTGTTACACAAATTTATTTTTTAGTATAGATATGTTTTTATGTGTTGATTGCCATTACTGTCGTTTTTCAGTATGATGATGACGACTAAATTTTAAAAAGAAAATAACTATGAGCATAACAACACAAGAAACCTATCGTAAGGAAGGCTATTTGCCGCAAAAAGTGAC‑ACTTTCAGAAGAAGTTGTATTTTTCACTAAAATTGA‑CAATGGCGAAATTAAAGTAAAATCCAATGATGAGGTCTTAGCCAACCTGAAAAAGATTATTGGTTAACAAGGCAAATTCTTTAGGCGTTTTTTCAATATCCGATTCTTTCCAATAGT  >  1/1401317‑1401786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑