Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,399,327	G→C	intergenic (+239747/+21610)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,399,327	0	G	C	100.0%	220.6 / NA	80	intergenic (+239747/+21610)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (36/44);  total (36/44)

TGTTCCGGTTTTAATTTGCCCTGTTTTTCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCAGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTGGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCGTTACCAATATCATATACTGCCGCATCATCTTTGGTTTCGTTACCCACCAATAAAT  >  1/1399089‑1399560                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           tgtTCCGGTTTTAATTTGCCCTGTTTTGCCGCCGTAGTTAAAACGCCGA‑GCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTcggcg                                                                                                                                                                                                                               <  1:574361/251‑1 (MQ=255)       ggTTTTAATTTGCCCTGTTTTTCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTaa                                                                                                                                                                                                                          >  1:193850/1‑251 (MQ=255)        gTTTTAATTTGCCCTGTTTTTCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCggg                                                                                                                                                                                                                             <  1:859129/247‑1 (MQ=255)             aaTTTGCCCTGTTTTTCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGt                                                                                                                                                                                                                    >  1:653813/1‑251 (MQ=255)               tttGCCCTGTTTTTCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTaa                                                                                                                                                                                                                  >  1:350793/1‑251 (MQ=255)                       gTTTTTCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGGAATCGGaaaa                                                                                                                                                                                                          >  1:504388/1‑250 (MQ=255)                           ttCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTa                                                                                                                                                                                                      <  1:575829/251‑1 (MQ=255)                                ccgTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAacac                                                                                                                                                                                                  <  1:752987/250‑1 (MQ=255)                                  gTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCg                                                                                                                                                                                                >  1:670227/1‑250 (MQ=255)                                    aGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGaa                                                                                                                                                                                                            >  1:743120/1‑236 (MQ=255)                                    aGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGATATTTAGAAATCGGAAATCTTAacaactgtt                                                                                                                                                                                             >  1:507646/1‑245 (MQ=255)                                                 aTGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCgg                                                                                                                                                                                <  1:684067/251‑1 (MQ=255)                                                  tGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGc                                                                                                                                                                               <  1:621884/251‑1 (MQ=255)                                                   gCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCgg                                                                                                                                                                                >  1:557086/1‑249 (MQ=255)                                                   gCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCgg                                                                                                                                                                                >  1:753806/1‑249 (MQ=255)                                                   gCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGGAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCgg                                                                                                                                                                                >  1:442377/1‑249 (MQ=255)                                                          ggTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTaccac                                                                                                                                                                       >  1:100073/1‑250 (MQ=255)                                                           gTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTaccac                                                                                                                                                                       <  1:776511/249‑1 (MQ=255)                                                               ggTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAg                                                                                                                                                                                                                     <  1:514150/200‑1 (MQ=255)                                                               ggTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAg                                                                                                                                                                 >  1:836702/1‑251 (MQ=255)                                                                gTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAacac                                                                                                                                                                                                  >  1:842231/1‑218 (MQ=255)                                                                gTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAac                                                                                                                                                                                                    <  1:872962/216‑1 (MQ=255)                                                                gTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGGAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCAt                                                                                                                                                                   >  1:83729/1‑248 (MQ=255)                                                                 ttAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCg                                                                                                                                                               <  1:6035/251‑1 (MQ=255)                                                                    aaTACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGGAATCGGTAATCCTAAAACCGCAATCGCCATTATCCGC‑TCACCACCCac                                                                                                                                                                   >  1:378173/1‑243 (MQ=255)                                                                          ggTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCa                                                                                                                                                      >  1:701240/1‑251 (MQ=255)                                                                          ggTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCa                                                                                                                                                      >  1:873404/1‑251 (MQ=255)                                                                          ggTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCa                                                                                                                                                      >  1:397262/1‑251 (MQ=255)                                                                                     gCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGtt                                                                                                                                           >  1:887763/1‑251 (MQ=255)                                                                                         cTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAg                                                                                                                                                                                                                     >  1:175094/1‑174 (MQ=255)                                                                                                      tcgtttGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAAttt                                                                                                                                                                                                                       <  1:368932/159‑1 (MQ=255)                                                                                                      tcgtttGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGc                                                                                                                           >  1:845059/1‑250 (MQ=255)                                                                                                                         tCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGatcat                                                                                                        >  1:834817/1‑250 (MQ=255)                                                                                                                          cAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCAt                                                                                                                                                                   <  1:859997/190‑1 (MQ=255)                                                                                                                          cAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTg                                                                                                                                               <  1:795540/210‑1 (MQ=255)                                                                                                                          cAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCg                                                                                                      >  1:87495/1‑251 (MQ=255)                                                                                                                           aataatGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGa                                                                                                     <  1:350958/251‑1 (MQ=255)                                                                                                                            ataatGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGAc                                                                                                    <  1:785412/251‑1 (MQ=255)                                                                                                                            ataatGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCCGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGAc                                                                                                    <  1:435355/251‑1 (MQ=255)                                                                                                                            ataatGCCGGTCACCGCCAAGCCAAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGAc                                                                                                    <  1:649195/251‑1 (MQ=255)                                                                                                                               atGCCGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAAt                                                                                                 >  1:488755/1‑251 (MQ=255)                                                                                                                                   cGGTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGc                                                                                             <  1:750708/251‑1 (MQ=255)                                                                                                                                    ggTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATcc                                                                                                                                                                                                        >  1:196962/1‑144 (MQ=255)                                                                                                                                    ggTCACCGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGGAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCAcccct                                                                                                                                                                   >  1:279480/1‑178 (MQ=255)                                                                                                                                         ccGCCAAGCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGaaa                                                                                       <  1:219516/251‑1 (MQ=255)                                                                                                                                                gCCGAAAATCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGGAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAAt                                                                                   >  1:585192/1‑248 (MQ=255)                                                                                                                                                      aaTCGGTTCGGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGc                                                                          <  1:319640/251‑1 (MQ=255)                                                                                                                                                               gggCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGcc                                                                                                                          >  1:671926/2‑194 (MQ=255)                                                                                                                                                                 gCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATccc                                                                <  1:138963/250‑1 (MQ=255)                                                                                                                                                                     aTCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAc                                                           <  1:738727/251‑1 (MQ=255)                                                                                                                                                                     aTCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAc                                                           <  1:400422/251‑1 (MQ=255)                                                                                                                                                                     aTCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAc                                                           <  1:298431/251‑1 (MQ=255)                                                                                                                                                                       cAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGaa                                                                                                                <  1:65001/196‑1 (MQ=255)                                                                                                                                                                       cAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAcca                                                         >  1:687207/1‑250 (MQ=255)                                                                                                                                                                         aTGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAccatt                                                       >  1:841041/1‑248 (MQ=255)                                                                                                                                                                             aaTGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTAcc                                                    <  1:718040/250‑1 (MQ=255)                                                                                                                                                                                 tCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAatat                                               <  1:67696/251‑1 (MQ=255)                                                                                                                                                                                  ccgccgGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCtgttg                                                                             <  1:49490/220‑1 (MQ=255)                                                                                                                                                                                       ggCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCg                                                                                                                                                                                 <  1:187821/116‑1 (MQ=255)                                                                                                                                                                                       ggCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGCTTTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGat                                                                                                           <  1:132132/186‑1 (MQ=255)                                                                                                                                                                                       ggCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTa                                                                                                                                          <  1:453870/154‑1 (MQ=255)                                                                                                                                                                                       ggCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTTTGCTGATGATCCCGATACCATTACCAATATCAtata                                         >  1:470405/1‑251 (MQ=255)                                                                                                                                                                                       ggCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAc                                                           <  1:123559/233‑1 (MQ=255)                                                                                                                                                                                       ggCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTAcc                                                    <  1:468477/240‑1 (MQ=255)                                                                                                                                                                                         cTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATAc                                        <  1:196704/250‑1 (MQ=255)                                                                                                                                                                                            gggCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATAc                                        >  1:83010/1‑247 (MQ=255)                                                                                                                                                                                                 aTGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATGACCAATATCATATAc                                        <  1:778887/242‑1 (MQ=255)                                                                                                                                                                                                        cTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAacac                                                                                                                                                                                                  <  1:125246/82‑1 (MQ=255)                                                                                                                                                                                                        cTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACGCCGCAATCGCCATTATCGGC‑TTACCTCCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTAcc                                                    <  1:12390/223‑1 (MQ=255)                                                                                                                                                                                                        cTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTAt                                                                                                                                                                                   >  1:335303/1‑97 (MQ=255)                                                                                                                                                                                                        cTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGaaa                                                                                                               <  1:205148/164‑1 (MQ=255)                                                                                                                                                                                                                cAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATAc                                        <  1:235288/227‑1 (MQ=255)                                                                                                                                                                                                                cAAGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATACTGCCGCATCATCTTTGGTTTCGtt                >  1:844563/1‑251 (MQ=255)                                                                                                                                                                                                                  aGCGAAACGACCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAc                                                           <  1:330807/206‑1 (MQ=255)                                                                                                                                                                                                                           aCCGCCGTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATACTGCCGCATCATCTTTGGTTTCGTTACCCACCAATa     >  1:604211/1‑251 (MQ=255)                                                                                                                                                                                                                              gccgTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTgatgat                                                                   <  1:188698/186‑1 (MQ=255)                                                                                                                                                                                                                              gccgTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATAc                                                           <  1:269923/194‑1 (MQ=255)                                                                                                                                                                                                                               ccgTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATACTGCCGCATCATCTTTGGTTTCGTTACCCACCAATAAAt  <  1:125729/250‑1 (MQ=255)                                                                                                                                                                                                                               ccgTCAACAATGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATACTGCCGCATCATCTTTGGTTTCGTTACCCACCAATAAAt  <  1:764507/250‑1 (MQ=255)                                                                                                                                                                                                                                   caacaaTGCGTTCGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCATTACCAATATCATATAc                                        >  1:308362/1‑208 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           TGTTCCGGTTTTAATTTGCCCTGTTTTTCCGCCGTAGTTAAAACGCCGATGCCCAGAGGTTTGGTTAAATACAGGTTGCAACCGGCTTCTGCGGAGGCGTTTCGTTTGACTTTTTCCGTATCAATAATGCCGGTCACCGCCAAGCCGAAAATCGGTTCAGGCGAATCAATGGAATGTCCGCCGGCTAGGGCAATGCCCGCTTCCTGACAAGCGAAACGACCGCCGTCAACAATGCGTTGGGCAACCTCGGCGGGTAATTTAGTAATCGGAAATCCTAACACCGCAATCGCCATTATCGGC‑TTACCACCCATAGCGAAAATGTCACTAATTGCGTTAGTTGCCGCAATACGCCCAAAATCGAAAGGATCATCGACAATTGGCATGAAAAAATCTGTTGTGCTGATGATCCCGATACCGTTACCAATATCATATACTGCCGCATCATCTTTGGTTTCGTTACCCACCAATAAAT  >  1/1399089‑1399560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑