Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,394,638	T→G	intergenic (+235058/+26299)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,394,638	0	T	G	100.0%	222.7 / NA	77	intergenic (+235058/+26299)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/49);  total (28/49)

GTTCACCCCTAACGGGGCCGTTGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGTCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATGCCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGCGGGTTAAGCTG  >  1/1394392‑1394872                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           gTTCACCCCTAACGGGGCCGTTGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTa                                                                                                                                                                                                                                        >  1:14481/1‑251 (MQ=255)      ccccTAACGGGGCCGTTGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAAtttt                                                                                                                                                                                                                                   >  1:872403/1‑251 (MQ=255)      ccccTAACGGGGCCGTTGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAAtttt                                                                                                                                                                                                                                   >  1:378818/1‑251 (MQ=255)                 gCCGTTGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAAtt                                                                                                                                                                                                                                     >  1:92519/1‑238 (MQ=255)                      tGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTgggg                                                                                                                                                                                                                   <  1:445481/251‑1 (MQ=255)                      tGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTgggg                                                                                                                                                                                                                   <  1:609463/251‑1 (MQ=255)                       ggCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGa                                                                                                                                                                                                                  >  1:371808/1‑251 (MQ=255)                       ggCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGa                                                                                                                                                                                                                  >  1:655897/1‑251 (MQ=255)                         cAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAg                                                                                                                                                                                                                <  1:859467/251‑1 (MQ=255)                            aGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTaa                                                                                                                                                                                                             >  1:875073/1‑251 (MQ=255)                               cAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATcc                                                                                                                                                                                                          <  1:144070/251‑1 (MQ=255)                                      aaaaaaaCGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGAc                                                                                                                                                                                                   <  1:616441/251‑1 (MQ=255)                                            aCGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAacac                                                                                                                                                                                             <  1:527587/251‑1 (MQ=255)                                                  ttttttGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAg                                                                                                                                                                                        <  1:405088/250‑1 (MQ=255)                                                  ttttttGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAg                                                                                                                                                                                        <  1:218385/250‑1 (MQ=255)                                                  ttttttGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGa                                                                                                                                                                                       <  1:119415/251‑1 (MQ=255)                                                   tttttGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGAt                                                                                                                                                                                      <  1:747576/251‑1 (MQ=255)                                                     tttGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGAtac                                                                                                                                                                                    <  1:106553/251‑3 (MQ=255)                                                            ccGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAAttt                                                                                                                                                                                                                                    <  1:887932/196‑1 (MQ=255)                                                               cACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCCCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTc                                                                                                                                                                          <  1:334816/251‑1 (MQ=255)                                                                 cTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCgg                                                                                                                                                                        <  1:881461/251‑1 (MQ=255)                                                                              aGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCAc                                                                                                                                                                                                                           <  1:214126/187‑1 (MQ=255)                                                                                gCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGcc                                                                                                                                                           <  1:104139/249‑1 (MQ=255)                                                                                     ccTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAg                                                                                                                                                                                        <  1:747000/215‑1 (MQ=255)                                                                                     ccTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAg                                                                                                                                                                               <  1:466890/224‑1 (MQ=255)                                                                                     ccTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGtca                                                                                                                                                    >  1:804488/1‑251 (MQ=255)                                                                                       ttAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGt                                                                                                                                                                            <  1:356776/225‑1 (MQ=255)                                                                                       ttAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGtcat                                                                                                                                                   >  1:108652/1‑250 (MQ=255)                                                                                         aGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGtcatca                                                                                                                                                 >  1:779971/1‑250 (MQ=255)                                                                                           aaaaaCGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGTGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATcac                                                                                                                                                <  1:249288/249‑1 (MQ=255)                                                                                            aaaaCGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACAtt                                                                                                                                             <  1:872559/251‑1 (MQ=255)                                                                                              aaCGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTAc                                                                                                                                           <  1:218026/251‑1 (MQ=255)                                                                                                         atTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGAGCCATGGGGCGGAGACGAAAATGGGGTCTTTTAAGCCGGATTGACTATCTAACGGACGAGCCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAGGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCgg                                                                                                                                 >  1:455413/1‑250 (MQ=255)                                                                                                                 aaGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGaa                                                                                                                        >  1:133180/1‑251 (MQ=255)                                                                                                                  aGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTAc                                                                                                                                           <  1:22384/231‑1 (MQ=255)                                                                                                                    aTTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAAt                                                                                                                       <  1:269049/249‑1 (MQ=255)                                                                                                                           ccccTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTAc                                                                                                                                           >  1:167489/1‑222 (MQ=255)                                                                                                                            cccTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAAt                                                                                                                       >  1:210065/1‑241 (MQ=255)                                                                                                                            cccTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGt                                                                                                             >  1:153091/1‑251 (MQ=255)                                                                                                                             ccTTAACGTGCAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTa                                                                                                            >  1:218878/1‑251 (MQ=255)                                                                                                                              cTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAg                                                                                                           <  1:769953/251‑1 (MQ=255)                                                                                                                                   cGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAAttt                                                                                                      <  1:89426/251‑1 (MQ=255)                                                                                                                                      gAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGAt                                                                                                                                                                                      >  1:869804/1‑168 (MQ=255)                                                                                                                                        aaTCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAg                                                                                                  >  1:70427/1‑250 (MQ=255)                                                                                                                                               tcttTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTAc                                                                                                                                           >  1:717840/1‑202 (MQ=255)                                                                                                                                                     gTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTg                                                                                     <  1:707849/250‑1 (MQ=255)                                                                                                                                                                cATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGttttt                                                                         >  1:302540/1‑251 (MQ=255)                                                                                                                                                                  tttCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTttgt                                                                                                                                                                                                                                >  1:432639/1‑98 (MQ=255)                                                                                                                                                                   ttCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCa                                                                      <  1:465228/251‑1 (MQ=255)                                                                                                                                                                       gCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAg                                                                    >  1:795240/1‑249 (MQ=255)                                                                                                                                                                              gcCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAg                                                           >  1:500036/1‑251 (MQ=255)                                                                                                                                                                               ccATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCa                                                                      <  1:308293/239‑1 (MQ=255)                                                                                                                                                                               ccATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAg                                                           <  1:319507/250‑1 (MQ=255)                                                                                                                                                                                cATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTg                                                         <  1:760947/251‑1 (MQ=255)                                                                                                                                                                                cATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTg                                                         <  1:360451/251‑1 (MQ=255)                                                                                                                                                                                    gggCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATc                                                      <  1:282079/250‑1 (MQ=255)                                                                                                                                                                                             agaaAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGc                                                                   >  1:64663/1‑228 (MQ=255)                                                                                                                                                                                                aaaTGGTGTCTTTTAAGCCGGATGGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGa                                                                                                                                                                                       >  1:365652/1‑109 (MQ=255)                                                                                                                                                                                                 aaTGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGgaga                                        <  1:223055/251‑1 (MQ=255)                                                                                                                                                                                                  aTGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGAc                                       <  1:812483/251‑1 (MQ=255)                                                                                                                                                                                                  aTGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGAc                                       <  1:131193/251‑1 (MQ=255)                                                                                                                                                                                                  aTGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGAc                                       <  1:809245/251‑1 (MQ=255)                                                                                                                                                                                                         cTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCAcc                                <  1:843041/251‑1 (MQ=255)                                                                                                                                                                                                                   ggATTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGAc                                       >  1:445392/1‑234 (MQ=255)                                                                                                                                                                                                                     aTTGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTc                     <  1:330951/250‑1 (MQ=255)                                                                                                                                                                                                                       tGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAAttt                                                                                                                     <  1:40550/152‑1 (MQ=255)                                                                                                                                                                                                                       tGACTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGG‑‑‑GTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCTCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAg               >  1:90828/1‑251 (MQ=255)                                                                                                                                                                                                                         aCTATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCAttt                 <  1:452736/250‑1 (MQ=255)                                                                                                                                                                                                                           tATCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAgg              <  1:494867/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGTTCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGc             <  1:157425/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGc             <  1:308531/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCTAACGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGc             <  1:790514/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGGACGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGCGGGTTa       <  1:869358/251‑1 (MQ=255)                                                                                                                                                                                                                                     aCGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGCGGGTTAAGc    <  1:35853/251‑1 (MQ=255)                                                                                                                                                                                                                                     aCGAACCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGCGGGTTAAGc    <  1:4249/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaCCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCgg                                                                                                                  >  1:49916/1‑138 (MQ=255)                                                                                                                                                                                                                                        aaCCCCAAATTCTTGGCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATACCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGCGGGTTAAGCTg  <  1:638478/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GTTCACCCCTAACGGGGCCGTTGGCAAAGCCAACGTTAAAAAAACGTCGTTTTTTGTGACCGCACTTTTTGGATGAAAGGCACGCCTTAGAAAAACGTGCCGATATTTTAATAAGATTCGCTCCCCTTAACGTGAAATCACTTCTTTGGTGAAAGATTCCATTTCAGCTTTGCGCCATGGGGCGGAGAAGAAAATGGTGTCTTTTAAGCCGGATTGACTATCTAACGGACGAACCCCAAATTCTTGTCTTAATTTTTGTTGCACTTCTGGGGAAGTAATCCAGTGGACAAACACTAAAGATGCCGCAGGGTTCGGTGTATTTTTCGCCACCGTCATCACATTACCGCCGCCCGGCATGCCGAATTTCGGAATGTAGAATTTTAAGCGGTCGGTGATTGCGCCTTGTTTTTGCAAGCTGAACAAGTGATCCTGCCAGGCGGAGACAATCACCAATTCGCCGTCATTTAGGCGGGTTAAGCTG  >  1/1394392‑1394872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑