Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,392,216	A→G	intergenic (+232636/+28721)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,392,216	0	A	G	100.0%	156.7 / NA	55	intergenic (+232636/+28721)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/25);  total (30/25)

GATTCATTAAACAATACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAAGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGACGATAAAAGCACGGTGATTTATGTGGGCAAGGCGAAAGATCTGAAAAAACGCTTATCCAGTTATTTTCGCAAGCAGTTAAGCAGCAAAA  >  1/1391973‑1392458                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   gATTCATTAAACAATACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGatat                                                                                                                                                                                                                                              <  1:544654/250‑1 (MQ=255)    tCATTAAACAATACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATAtttt                                                                                                                                                                                                                                           >  1:615122/1‑250 (MQ=255)     cATTAAACAATACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATAtttt                                                                                                                                                                                                                                           >  1:526975/1‑249 (MQ=255)         aaaCAATACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATAttttcttttt                                                                                                                                                                                                                                     >  1:880611/1‑251 (MQ=255)            cAATACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATAttttctttttt                                                                                                                                                                                                                                    >  1:726052/1‑249 (MQ=255)               tACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTa                                                                                                                                                                                                                               >  1:499114/1‑251 (MQ=255)               tACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTa                                                                                                                                                                                                                               >  1:43092/1‑251 (MQ=255)                aCGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTaa                                                                                                                                                                                                                              >  1:557341/1‑251 (MQ=255)                  gTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAg                                                                                                                                                                                                                            >  1:772377/1‑251 (MQ=255)                  gTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAg                                                                                                                                                                                                                            >  1:271891/1‑251 (MQ=255)                               cGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAatat                                                                                                                                                                                                               <  1:383620/251‑1 (MQ=255)                                  cTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAAt                                                                                                                                                                                                            <  1:272073/251‑1 (MQ=255)                                    gcgcTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATg                                                                                                                                                                                                           >  1:115120/1‑250 (MQ=255)                                    gcgcTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATg                                                                                                                                                                                                           >  1:732416/1‑250 (MQ=255)                                     cgcTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATAttttc                                                                                                                                                                                                                                          >  1:101580/1‑218 (MQ=255)                                               caTCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCAtt                                                                                                                                                                                               >  1:112576/1‑251 (MQ=255)                                                        aCTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAg                                                                                                                                                                                      <  1:403971/251‑1 (MQ=255)                                                           ggAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACg                                                                                                                                                                                                                    <  1:153528/218‑1 (MQ=255)                                                            gAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAAc                                                                                                                                                                                   <  1:571198/250‑1 (MQ=255)                                                               cAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGc                                                                                                                                                                                                          <  1:577484/224‑1 (MQ=255)                                                               cAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACtat                                                                                                                                                                                        >  1:459180/1‑242 (MQ=255)                                                                 aTTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAatat                                                                                                                                                                                                               <  1:601115/217‑1 (MQ=255)                                                                 aTTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAAc                                                                                                                                                                                   >  1:470443/1‑245 (MQ=255)                                                                         tGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTtaattaat                                                                                                                                                                     >  1:310052/1‑251 (MQ=255)                                                                                    ggCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCaaa                                                                                                                                                                                                    <  1:481304/209‑1 (MQ=255)                                                                                     gCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGAc                                                                                                                                                          <  1:71244/250‑1 (MQ=255)                                                                                     gCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGAc                                                                                                                                                          <  1:211317/250‑1 (MQ=255)                                                                                                 aTGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTACAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCaaa                                                                                                                                             >  1:445845/1‑251 (MQ=255)                                                                                                 aTGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGc                                                                                                                                                                                                          <  1:437532/190‑1 (MQ=255)                                                                                                       aaCTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTc                                                                                                                                       <  1:557264/251‑1 (MQ=255)                                                                                                           cGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGCCGTTAACGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTACAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAAt                                                                                                                                         >  1:508729/1‑251 (MQ=255)                                                                                                           cGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGAc                                                                                                                                                          <  1:353237/228‑1 (MQ=255)                                                                                                           cGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCg                                                                                                                                   >  1:795617/1‑251 (MQ=255)                                                                                                           cGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCg                                                                                                                                   >  1:70303/1‑251 (MQ=255)                                                                                                               aaaGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCaa                                                                                                                               >  1:780658/1‑251 (MQ=255)                                                                                                                    agTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACg                                                                                                                                                                                                                    <  1:734176/161‑1 (MQ=255)                                                                                                                     gTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTAc                                                                                                                          >  1:790469/1‑250 (MQ=255)                                                                                                                     gTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTAc                                                                                                                          >  1:325882/1‑250 (MQ=255)                                                                                                                               tGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTAcc                                                                                                                <  1:552883/250‑1 (MQ=255)                                                                                                                                      ggATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGa                                                                                                                                                           >  1:185959/1‑200 (MQ=255)                                                                                                                                           cccccGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCg                                                                                                               <  1:241721/239‑1 (MQ=255)                                                                                                                                                    aTCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGa                                                                                           <  1:246795/250‑1 (MQ=255)                                                                                                                                                       tGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGACGAt                                                                                       <  1:82154/251‑1 (MQ=255)                                                                                                                                                        ggAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGc                                                                                                                                                                                                          >  1:145064/1‑135 (MQ=255)                                                                                                                                                             aaGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAAtc                                                                                                                                                                    <  1:837805/168‑1 (MQ=255)                                                                                                                                                                                                    aaCGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAAc                                                                                                                                                                                   <  1:565243/120‑1 (MQ=255)                                                                                                                                                                                                      cGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGAc                                                                                          <  1:397818/207‑1 (MQ=255)                                                                                                                                                                                                         aTTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGACGATAAAAGCACGGTGATTTATGTGGGCAAGGCGAAAGATCTGaaaaa                                           >  1:761476/1‑251 (MQ=255)                                                                                                                                                                                                                                     cTAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGACGATAAAAGCACGGTGATTTATGTGGGCAAGGCGAAAGATCTGAAAAAACGCTTATCCAGTTATTTTCGCAAGCAg               <  1:77960/251‑1 (MQ=255)                                                                                                                                                                                                                                      tAACTCCTCTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTc                                                                                                                                                                                 <  1:423501/88‑1 (MQ=255)                                                                                                                                                                                                                                         ctcctcTTTGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGACGATAAAAGCACGGTGATTTAtg                                                                    >  1:616507/1‑194 (MQ=255)                                                                                                                                                                                                                                                ttGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGAc                                                                                                                                                          >  1:628111/1‑101 (MQ=255)                                                                                                                                                                                                                                                ttGCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCgta                                                                                                  <  1:508757/157‑1 (MQ=255)                                                                                                                                                                                                                                                  gCTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGACGATAAAAGCACGGTGATTTATGTGGGCAAGGCGAAAGATCTGAAAAAACGCTTATCCAGTTATTTTCGCAAGCAGTTAAGCAGCaaaa  >  1:725861/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cTAAAGAGGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACtat                                                                                                                                                                                        >  1:172280/1‑68 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   GATTCATTAAACAATACGTACAGTGGCAGCCGACTGCGCTGGAACACATCGAAAAACTGGAACAATTACGCGTGCTTTGGCATGGCGAAAAAATCCATGTAGAACTCGCTAAAGAAGTGCCTGCCGTGGGTGTGGATACCCCCGAAGATCTGGAAAAAGTGCGGTCGATTTTATCGGAGAAATGC‑‑‑‑‑‑CGTTAACGTATTTTGCACCTAAATTGACCAATCTCCCCTAACTCCTCTTTGCTAAAGAAGGATATTTTCTTTTTTCTTTAAAGATTAGACGAATATAATGCTTCAAATCATTTCACTATAGAACTCGATTTAATTAATCTCATTATGACTTTCGACGCCAAAAAATTCCTCGCCAACGTACCGCACTTACCGGGCGTTTATCGTATGTATGACGATAAAAGCACGGTGATTTATGTGGGCAAGGCGAAAGATCTGAAAAAACGCTTATCCAGTTATTTTCGCAAGCAGTTAAGCAGCAAAA  >  1/1391973‑1392458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑