Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,391,711 A→G intergenic (+232131/+29226) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,391,7110AG98.4% 173.9 / NA 64intergenic (+232131/+29226)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (32/31);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAACACGTTTTTGAACAAGCCAAACAATCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCATTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAATATTTACGTCACATCGGCA  >  1/1391481‑1391954
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
ttCAACACGTTTTTGAACAAGCCAAACAATCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCg                                                                                                                                                                                                                                 >  1:501495/1‑251 (MQ=255)
   aaCACGTTTTTGAACAAGCCAAACAATCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGCAACAAGt                                                                                                                                                                                                                                     >  1:868176/1‑244 (MQ=255)
      ccgttttaaaaCAAGCAAAACAACCGGGTGCCGACCGCGTCATTACCCCCCCCGATAACGAATTGGGGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAAc                                                                                                                                                                                                                           <  1:759217/242‑1 (MQ=255)
       gttttttGAACAAGCCAAACAATCCGGTGCCGCCCGCGTCATTATCGCCACCGATAACGAATGGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAAc                                                                                                                                                                                                                           <  1:90981/248‑1 (MQ=255)
        gTTTTTGAACAAGCCAAACAATCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAAc                                                                                                                                                                                                                           >  1:415414/1‑249 (MQ=255)
         tttttGAACAAGCCAAACAATCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAAc                                                                                                                                                                                                                           >  1:603961/1‑248 (MQ=255)
                  aacccAAACAACCCGGTGCCGCCCGCGTAATTACCCCCCCCGATAACGAATTGGGGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATAc                                                                                                                                                                                                               <  1:806276/248‑1 (MQ=255)
                  aaccaaaaCAACCCGGTGCCGCCCGCGTCATTACCCCCCCCGATAACGAATGGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATAc                                                                                                                                                                                                               <  1:781112/246‑1 (MQ=255)
                    gCCAAACAATCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATAcc                                                                                                                                                                                                              >  1:823464/1‑250 (MQ=255)
                    ccaaaaCAACCCGGTGCCGCCCGCGTCATTATCGCCCCCGATAACGAATGGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATAccc                                                                                                                                                                                                             <  1:343817/248‑1 (MQ=255)
                           aaTCCGGTGCCGCCCGCTTCATTATCCCCACCAAAAAGGATTTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAAc                                                                                                                                                                                                      <  1:868895/251‑1 (MQ=255)
                           aaTCCGGTGCCGCCCGCGTCATTACCGCCACCGAAAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAAc                                                                                                                                                                                                      <  1:821645/251‑1 (MQ=255)
                            accgggggCCGACGGCGTCATTACCGCCCCCGAAAACGAAGTGGTGGAAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGTATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACa                                                                                                                                                                                                     <  1:448830/244‑1 (MQ=255)
                            aTCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTTGCAAAATACCCTGTGAACa                                                                                                                                                                                                     >  1:805891/1‑251 (MQ=255)
                            aTCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACa                                                                                                                                                                                                     >  1:603107/1‑251 (MQ=255)
                            aTCCGGTGCCGACCGCGCCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACa                                                                                                                                                                                                     >  1:45459/1‑251 (MQ=255)
                              ccGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATg                                                                                                                                                                                                   >  1:199111/1‑251 (MQ=255)
                                           cgTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCgg                                                                                                                                                                                      >  1:149433/1‑251 (MQ=255)
                                           cgTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCgg                                                                                                                                                                                      >  1:139232/1‑251 (MQ=255)
                                             tCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTAt                                                                                                                                                                                                                                                 >  1:278985/1‑190 (MQ=255)
                                                 taccgcccccgaaaaggagtggggggCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAACTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAGGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTaaaaa                                                                                                                                                                                <  1:443080/227‑1 (MQ=255)
                                                    cGCCACCGAAAACGAATTGGTGGAACCCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATtg                                                                                                                                                                             <  1:351418/251‑1 (MQ=255)
                                                           gaaaaCGAATGGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCgg                                                                                                                                                                                      <  1:242670/232‑1 (MQ=255)
                                                            aTAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGAcgcg                                                                                                                                                                     >  1:895635/1‑251 (MQ=255)
                                                             tAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGa                                                                                                                                                                    >  1:350340/1‑251 (MQ=255)
                                                             tAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCATTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGa                                                                                                                                                                    >  1:445441/1‑251 (MQ=255)
                                                                 gAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACa                                                                                                                                                                                                     >  1:827147/1‑214 (MQ=255)
                                                                  atttGGTGGAAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGa                                                                                                                                                                <  1:255042/248‑1 (MQ=255)
                                                                  aatgggtgcCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGa                                                                                                                                                                <  1:55726/246‑1 (MQ=255)
                                                                  aaTTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGaa                                                                                                                                                               >  1:538875/1‑251 (MQ=255)
                                                                                         aaTTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTaaa                                                                                                                                        <  1:290586/251‑1 (MQ=255)
                                                                                           tttCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAg                                                                                                                                      <  1:490006/251‑1 (MQ=255)
                                                                                            ttCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAGGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGt                                                                                                                                     <  1:106069/251‑1 (MQ=255)
                                                                                                gggCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTg                                                                                                                                    >  1:200924/1‑248 (MQ=255)
                                                                                                        gtgtGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGAcgcg                                                                                                                                                                     >  1:468809/1‑207 (MQ=255)
                                                                                                          gtgCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACaaaa                                                                                                                       >  1:721049/1‑251 (MQ=255)
                                                                                                          gtgCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACACTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTg                                                                                                                                    >  1:879398/1‑238 (MQ=255)
                                                                                                               tGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACgg                                                                                                                   >  1:235030/1‑250 (MQ=255)
                                                                                                                           aCCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTAt                                                                                                      <  1:133523/251‑1 (MQ=255)
                                                                                                                           aCCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTAt                                                                                                      <  1:466936/251‑1 (MQ=255)
                                                                                                                            ccACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTAtt                                                                                                     <  1:317196/251‑1 (MQ=255)
                                                                                                                             cacaATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTAttt                                                                                                    <  1:365693/251‑1 (MQ=255)
                                                                                                                                  ttCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGACTTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGc                                                                                                           >  1:130103/1‑239 (MQ=255)
                                                                                                                                              aCGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAATTTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTc                                                                                   <  1:358297/251‑1 (MQ=255)
                                                                                                                                                              gtggAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGa                                                                      >  1:122648/1‑248 (MQ=255)
                                                                                                                                                              gtggAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCa                                                                   >  1:312701/1‑251 (MQ=255)
                                                                                                                                                                ggAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAAt                                                                 >  1:388352/1‑251 (MQ=255)
                                                                                                                                                                  aaaaaCTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAAtt                                                                <  1:222238/250‑1 (MQ=255)
                                                                                                                                                                  aaaaaCTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAAtt                                                                <  1:633870/250‑1 (MQ=255)
                                                                                                                                                                       cTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCgcgc                                                                                           <  1:502934/218‑1 (MQ=255)
                                                                                                                                                                                      gaTGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACgg                                            >  1:797493/1‑250 (MQ=255)
                                                                                                                                                                                          aaaTTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGAc                                                   <  1:174263/239‑1 (MQ=255)
                                                                                                                                                                                                   tCAACATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCt                               <  1:72584/250‑1 (MQ=255)
                                                                                                                                                                                                      aCATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGc                                <  1:84992/246‑1 (MQ=255)
                                                                                                                                                                                                      aCATTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAAt                           >  1:61940/1‑251 (MQ=255)
                                                                                                                                                                                                        aTTCAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATAc                         <  1:62773/251‑1 (MQ=255)
                                                                                                                                                                                                           cAAGGCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGa                      >  1:107544/1‑251 (MQ=255)
                                                                                                                                                                                                              ggCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAata                   >  1:458575/1‑251 (MQ=255)
                                                                                                                                                                                                               gCGACGAACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAatat                  >  1:201993/1‑251 (MQ=255)
                                                                                                                                                                                                                cgacgaACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAatat                  <  1:314633/250‑1 (MQ=255)
                                                                                                                                                                                                                 gacgaACCGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAATAttt                >  1:542001/1‑251 (MQ=255)
                                                                                                                                                                                                                       ccGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAATATTTACGTca          >  1:201377/1‑251 (MQ=255)
                                                                                                                                                                                                                        cGCTCATTCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAATATTTACGTc           <  1:825075/249‑1 (MQ=255)
                                                                                                                                                                                                                               tCCGCCCGTTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAATATTTACGTCACATCGGCa  <  1:564111/251‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
TTCAACACGTTTTTGAACAAGCCAAACAATCCGGTGCCGACCGCGTCATTATCGCCACCGATAACGAATTGGTGGCAACCGCCGCAAGAAATTTCGGGGCGGAAGTGTGCATGACTGCCAAAAACCACAATTCCGGCACGGAACGCCTGGCGGAAGTGGTGGAAAAACTGGTGCTGCCCGACGATGAAATTATCGTCAACATTCAAGGCGACGAACCGCTCATTCCGCCCATTATCGTGAAACAAGTGGCGGAAAACCTGGCAAAATACCCTGTGAACATGGCAAGCCTTGCGGTAAAAATTGATGACGCGAAAGAATTGTTTAATCCCAACGTGGTAAAAGTGCTGACCGACAAAAACGGCTATGTGCTTTATTTCTCCCGCGCCGTGATTCCCTGGGATCGCGATCAATTCGCACAAACGGACGATACGGCAAAGTTACGGCTGAATACCGAATATTTACGTCACATCGGCA  >  1/1391481‑1391954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑