Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,390,604	A→G	intergenic (+231024/+30333)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,390,604	0	A	G	100.0%	220.6 / NA	79	intergenic (+231024/+30333)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/43);  total (36/43)

TCTTTCCGTCGGCGGTAACGGCAAAACACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATACCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGACGCGGTGATGACATTGCAACCACAGTATGC  >  1/1390365‑1390847                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    tCTTTCCGTCGGCGGTAACGGCAAAACACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAac                                                                                                                                                                                                                                          <  1:403881/251‑1 (MQ=255) tCTTTCCGTCGGCGGTAACGGCAAAACACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAac                                                                                                                                                                                                                                          <  1:634325/251‑1 (MQ=255) tCTTTCCGTCGGCGGTAACGGCAAAACACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAac                                                                                                                                                                                                                                          <  1:244152/251‑1 (MQ=255)            gcggTAACGGCAAAACACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATg                                                                                                                                                                                                                                <  1:900136/250‑1 (MQ=255)                        aaaCACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATc                                                                                                                                                                                                                   <  1:717759/251‑1 (MQ=255)                        aaaCACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATc                                                                                                                                                                                                                   <  1:188548/251‑1 (MQ=255)                                   gTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACgg                                                                                                                                                                                                        >  1:167456/1‑251 (MQ=255)                                     gATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACgg                                                                                                                                                                                                        >  1:203408/1‑249 (MQ=255)                                          ggttggtACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCaa                                                                                                                                                                                                 >  1:403128/1‑251 (MQ=255)                                          ggttggtACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCaa                                                                                                                                                                                                 >  1:632800/1‑251 (MQ=255)                                          ggttggtACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCaa                                                                                                                                                                                                 >  1:785610/1‑251 (MQ=255)                                               gtACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAg                                                                                                                                                                                                                 <  1:618520/230‑1 (MQ=255)                                               gtACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATta                                                                                                                                                                                            >  1:206920/1‑251 (MQ=255)                                               gtACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATta                                                                                                                                                                                            >  1:549426/1‑251 (MQ=255)                                                    ggAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATc                                                                                                                                                                                                                   >  1:287757/1‑223 (MQ=255)                                                         tCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCTGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACaa                                                                                                                                                                                  <  1:428081/251‑1 (MQ=255)                                                             cAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAAc                                                                                                                                                                                 <  1:283964/248‑1 (MQ=255)                                                                    ggTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGa                                                                                                                                                                       >  1:117156/1‑251 (MQ=255)                                                                      tttAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAg                                                                                                                                                                     <  1:736379/251‑1 (MQ=255)                                                                      tttAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCCGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAg                                                                                                                                                                     <  1:437904/251‑1 (MQ=255)                                                                                 gCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCg                                                                                                                                                                        <  1:47656/237‑1 (MQ=255)                                                                                 gCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATg                                                                                                                                                          >  1:824804/1‑251 (MQ=255)                                                                                  cGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATg                                                                                                                                                          <  1:436138/250‑1 (MQ=255)                                                                                    tGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATg                                                                                                                                                          <  1:216106/248‑1 (MQ=255)                                                                                    tGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGAc                                                                                                                                                       <  1:78303/251‑1 (MQ=255)                                                                                              gggCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATc                                                                                                                                                                                                                   >  1:4883/1‑181 (MQ=255)                                                                                              gggCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGt                                                                                                                                              <  1:882260/250‑1 (MQ=255)                                                                                              gggCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTg                                                                                                                                             >  1:640520/1‑251 (MQ=255)                                                                                               ggCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTg                                                                                                                                             <  1:464721/250‑1 (MQ=255)                                                                                               ggCTACGGCAGCCAAGCC‑AACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTgg                                                                                                                                            <  1:324710/250‑1 (MQ=255)                                                                                                           caagccaaACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCTGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACgg                                                                                                                                <  1:454455/251‑1 (MQ=255)                                                                                                           caagccaaACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACgg                                                                                                                                <  1:485405/251‑1 (MQ=255)                                                                                                              gccaaACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGttt                                                                                                                             >  1:246068/1‑251 (MQ=255)                                                                                                                   aCATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGAc                                                                                                                                                       >  1:368198/1‑220 (MQ=255)                                                                                                                   aCATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTAt                                                                                                                        <  1:507893/251‑1 (MQ=255)                                                                                                                      ttATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATtgc                                                                                                                     <  1:495918/251‑1 (MQ=255)                                                                                                                       tATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATtgcc                                                                                                                    <  1:96161/251‑1 (MQ=255)                                                                                                                          ccTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGa                                                                                                                                                                                                              <  1:162854/158‑1 (MQ=255)                                                                                                                               gttgGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGa                                                                                                             >  1:825320/1‑250 (MQ=255)                                                                                                                                 tgGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCg                                                                                                                                                                        >  1:640406/1‑189 (MQ=255)                                                                                                                                  ggTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGt                                                                                                         >  1:147478/1‑251 (MQ=255)                                                                                                                                   gTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGt                                                                                                         <  1:459471/250‑1 (MQ=255)                                                                                                                                           gccCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATg                                                                                                                                                          >  1:76094/1‑193 (MQ=255)                                                                                                                                           gccCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGt                                                                                                >  1:38790/1‑251 (MQ=255)                                                                                                                                           gccCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGCTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACg                                                                                                                                               >  1:277151/1‑204 (MQ=255)                                                                                                                                             ccAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGAGGTCATTATCAGCGATGACGGCTTGCAACATTATAAAtt                                                                                                                                                                                      <  1:369526/163‑1 (MQ=255)                                                                                                                                                 cGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCaa                                                                                          <  1:37302/251‑1 (MQ=255)                                                                                                                                                   aTCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGAc                                                                                                            <  1:245650/231‑1 (MQ=255)                                                                                                                                                   aTCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAg                                                                                         >  1:256894/1‑250 (MQ=255)                                                                                                                                                     ccTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGc                                                                                        <  1:353939/249‑1 (MQ=255)                                                                                                                                                        gTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAac                                                                                                                                                                                                                                          <  1:802331/100‑1 (MQ=255)                                                                                                                                                                       gAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACaa                                                                                                                                                                                  >  1:578509/1‑141 (MQ=255)                                                                                                                                                                         gCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACg                                                                                                                                               >  1:590845/1‑174 (MQ=255)                                                                                                                                                                          cccGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAac                                                                                                                                                                                                                                          >  1:415536/1‑82 (MQ=255)                                                                                                                                                                          cccGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAg                                                                                                                                                                     <  1:123353/151‑1 (MQ=255)                                                                                                                                                                             gtgtTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAAt                                                                                                                                                                                       <  1:490048/130‑1 (MQ=255)                                                                                                                                                                             gtgtTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTcgtcgt                                                              >  1:607902/1‑251 (MQ=255)                                                                                                                                                                               gtTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACgg                                                                                                                                <  1:505447/183‑1 (MQ=255)                                                                                                                                                                                  gATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATc                                                                             >  1:171703/1‑231 (MQ=255)                                                                                                                                                                                  gATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTa                                                         >  1:634985/1‑251 (MQ=255)                                                                                                                                                                                       ccACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATgg                                                     <  1:878007/250‑1 (MQ=255)                                                                                                                                                                                         aCCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTACCGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGgcg                                                   >  1:451355/1‑250 (MQ=255)                                                                                                                                                                                         aCCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACACACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGgcg                                                   >  1:105951/1‑250 (MQ=255)                                                                                                                                                                                          cccGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGc                                                                                                                                                                                                       >  1:325744/1‑101 (MQ=255)                                                                                                                                                                                             gTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTa                                                         <  1:332930/240‑1 (MQ=255)                                                                                                                                                                                                 ccAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAAcc                                          <  1:82654/251‑1 (MQ=255)                                                                                                                                                                                                      cgcCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATAc                                     <  1:417723/251‑1 (MQ=255)                                                                                                                                                                                                        cccGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAg                                   <  1:99909/251‑1 (MQ=255)                                                                                                                                                                                                          cGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCtt                                                                                                                          >  1:467234/1‑162 (MQ=255)                                                                                                                                                                                                          cGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCg                                 >  1:517610/1‑251 (MQ=255)                                                                                                                                                                                                           gTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATtg                                                                                                                      <  1:466116/165‑1 (MQ=255)                                                                                                                                                                                                            tttGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGa                                >  1:707786/1‑250 (MQ=255)                                                                                                                                                                                                            tttGTATCTCACCCAACCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGa                                >  1:273704/1‑250 (MQ=255)                                                                                                                                                                                                                           aCCGACAACAAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGcac                                                                                                                                                   <  1:298705/120‑1 (MQ=255)                                                                                                                                                                                                                               acaacaAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGACGCGGTGATGACATTGCaa             >  1:229822/1‑250 (MQ=255)                                                                                                                                                                                                                               acaacaAGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGAGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGACGCGGTGATGACATTGCAAc            <  1:900541/251‑1 (MQ=255)                                                                                                                                                                                                                                caacaaGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGAcc                                                                                                           >  1:719554/1‑155 (MQ=255)                                                                                                                                                                                                                                  acaaGCCGTTGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGACGCGGTGATGACATTGCAACcac         <  1:786640/251‑1 (MQ=255)                                                                                                                                                                                                                                          ttGAATGCCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGACGCGGTGATGACATTGCAACCACAGTATGc  <  1:874390/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    TCTTTCCGTCGGCGGTAACGGCAAAACACCGGCAGTGATTTGGTTGGTACAGGAACTCAGCAAGCGCGGTTTAAAAGTCGGCGTGATTTCACGGGGCTACGGCAGCCAAGCCAAACATTATCCTTTGTTGGTTACGCCGCCCAGCGATCCTGTAGAAGCCGGCGATGAGCCCGTGTTGATCGCCACCCGTACCCAAGCGCCCGTTTGTATCTCACCCAACCGACAACAAGCCGTTGAATACCTGTTAGAACACGCCCAATGCGATGTCATTATCAGCGATGACGGCTTGCAACATTATAAATTACAACGTGATATCGAAGTCGTCATCATGGACGCACAACGTGGTCTGGGTAACGGTTTCTTATTGCCTGCCGGACCGTTGCGCGAGTTGCCAAGCCGTTTAAAATCTGTCGATTTCGTCGTTACTAATGGCGCGGAAAACCAATACAGCGACGCGGTGATGACATTGCAACCACAGTATGC  >  1/1390365‑1390847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑