Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,376,726	T→C	intergenic (+217146/+44211)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,376,726	0	T	C	100.0%	207.0 / NA	74	intergenic (+217146/+44211)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/34);  total (40/34)

AAACCTGGAGCGATAAGGAATTGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCTCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGATGACCACCCTAAGCA  >  1/1376487‑1376969                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    aaaCCTGGAGCGATAAGGAATTGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACgg                                                                                                                                                                                                                                           <  1:540417/250‑1 (MQ=255)  aaCCTGGAGCGATAAGGAATTGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGgcg                                                                                                                                                                                                                                         >  1:496674/1‑251 (MQ=255)         aGCGATAAGGAATTGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTAtg                                                                                                                                                                                                                                   <  1:575421/250‑1 (MQ=255)                  gAATTGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTAtg                                                                                                                                                                                                                                   <  1:43438/241‑1 (MQ=255)                    aTTGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATcc                                                                                                                                                                                                                         <  1:173816/249‑1 (MQ=255)                     ttGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGt                                                                                                                                                                                                                      <  1:225652/251‑1 (MQ=255)                            gTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCg                                                                                                                                                                                                                               >  1:780962/1‑235 (MQ=255)                            gTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCgatgat                                                                                                                                                                                                               >  1:409264/1‑251 (MQ=255)                             tCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCgatgat                                                                                                                                                                                                               <  1:375296/250‑1 (MQ=255)                                        ccGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCAtt                                                                                                                                                                                                   >  1:629134/1‑251 (MQ=255)                                                         gATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGcc                                                                                                                                                                                   <  1:565207/250‑1 (MQ=255)                                                         gATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCa                                                                                                                                                                                  >  1:641860/1‑251 (MQ=255)                                                         gATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCa                                                                                                                                                                                  >  1:686132/1‑251 (MQ=255)                                                            gtgtTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCg                                                                                                                                                                                                                               >  1:685590/1‑203 (MQ=255)                                                             tgtTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAAcgt                                                                                                                                                                              >  1:109206/1‑251 (MQ=255)                                                                        cAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCg                                                                                                                                                                    <  1:418259/250‑1 (MQ=255)                                                                        cAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCg                                                                                                                                                                    <  1:877839/250‑1 (MQ=255)                                                                          aGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGa                                                                                                                                                                 >  1:544816/1‑251 (MQ=255)                                                                            gACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGAtt                                                                                                                                                               >  1:606394/1‑251 (MQ=255)                                                                                        gCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGAt                                                                                                                                                   >  1:83801/1‑251 (MQ=255)                                                                                        gCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGAt                                                                                                                                                   >  1:538770/1‑251 (MQ=255)                                                                                                ccGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCAc                                                                                                                                           <  1:1274/251‑1 (MQ=255)                                                                                                ccGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCAc                                                                                                                                           <  1:584511/251‑1 (MQ=255)                                                                                                ccGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCAc                                                                                                                                           <  1:816104/251‑1 (MQ=255)                                                                                                ccGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCAc                                                                                                                                           <  1:838777/251‑1 (MQ=255)                                                                                                     gAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTg                                                                                                                                       >  1:727556/1‑250 (MQ=255)                                                                                                      aaCCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCAc                                                                                                                                           <  1:823297/245‑1 (MQ=255)                                                                                                                ttCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCGCATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCgaaag                                                                                                                                <  1:195572/246‑1 (MQ=255)                                                                                                                ttCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAAt                                                                                                                            >  1:830600/1‑250 (MQ=255)                                                                                                                            gcgcTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACt                                                                                                                >  1:575349/1‑250 (MQ=255)                                                                                                                               cTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCAc                                                                                                                                           <  1:793965/220‑1 (MQ=255)                                                                                                                               cTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCaaa                                                                                                            >  1:712168/1‑251 (MQ=255)                                                                                                                               cTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCaaa                                                                                                            >  1:425389/1‑251 (MQ=255)                                                                                                                                 tATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACtgagttt                                                                                                          >  1:390851/1‑245 (MQ=255)                                                                                                                                 tATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCaaaaa                                                                                                          <  1:384686/251‑1 (MQ=255)                                                                                                                                            tATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCAttt                                                                                               >  1:221260/1‑251 (MQ=255)                                                                                                                                                gACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGa                                                                                                                                                                 >  1:723183/1‑181 (MQ=255)                                                                                                                                                gACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGc                                                                                                                                             <  1:897014/201‑1 (MQ=255)                                                                                                                                                       tGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCCGCAAACTCAAAAATGCCGGCATTTTGTCTTACaa                                                                                     <  1:705134/250‑1 (MQ=255)                                                                                                                                                               aaTATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTa                                                                            >  1:506047/1‑251 (MQ=255)                                                                                                                                                                atatGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGc                                                                                                                                                                        >  1:450232/1‑158 (MQ=255)                                                                                                                                                                 tatGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTAtt                                                                          >  1:398013/1‑251 (MQ=255)                                                                                                                                                                          ttGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACaa                                                                                     >  1:562681/1‑231 (MQ=255)                                                                                                                                                                            gCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGAt                                                               >  1:564415/1‑251 (MQ=255)                                                                                                                                                                            gCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGAt                                                               >  1:745835/1‑251 (MQ=255)                                                                                                                                                                               gTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCACACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATaaa                                                            >  1:690518/1‑251 (MQ=255)                                                                                                                                                                                 gCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACa                                                          >  1:510481/1‑251 (MQ=255)                                                                                                                                                                                   aTTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAg                                                        >  1:767272/1‑251 (MQ=255)                                                                                                                                                                                   aTTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAg                                                        >  1:477342/1‑251 (MQ=255)                                                                                                                                                                                      gCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTg                                                                                             <  1:818645/211‑1 (MQ=255)                                                                                                                                                                                      gCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGa                                                      >  1:563107/1‑250 (MQ=255)                                                                                                                                                                                          aTCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACaa                                                         >  1:229177/1‑243 (MQ=255)                                                                                                                                                                                           ccGATCACATTATGTCTTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCATCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGt                                                <  1:867811/250‑1 (MQ=255)                                                                                                                                                                                                  caTTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGAcc                                         <  1:490393/251‑1 (MQ=255)                                                                                                                                                                                                  caTTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGAcc                                         <  1:36092/251‑1 (MQ=255)                                                                                                                                                                                                  caTTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGAcc                                         <  1:60103/251‑1 (MQ=255)                                                                                                                                                                                                  caTTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGAc                                          >  1:187744/1‑250 (MQ=255)                                                                                                                                                                                                      aTGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAACAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCg                                     >  1:441933/1‑251 (MQ=255)                                                                                                                                                                                                          cGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGa                                  >  1:617159/1‑250 (MQ=255)                                                                                                                                                                                                               ttGCGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATcc                            <  1:297946/251‑1 (MQ=255)                                                                                                                                                                                                                  cGTTTATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAACTGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTa                         >  1:430956/1‑251 (MQ=255)                                                                                                                                                                                                                    tttATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTAc                        <  1:879974/250‑1 (MQ=255)                                                                                                                                                                                                                    tttATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTAc                        <  1:840437/250‑1 (MQ=255)                                                                                                                                                                                                                    tttATGGTGGATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTAc                        <  1:700839/250‑1 (MQ=255)                                                                                                                                                                                                                         ggtggATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGt                                                <  1:350408/221‑1 (MQ=255)                                                                                                                                                                                                                          gtggATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGa                 >  1:224492/1‑251 (MQ=255)                                                                                                                                                                                                                            ggATCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGAc                                          >  1:498160/1‑224 (MQ=255)                                                                                                                                                                                                                              aTCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGCGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGAc                                          >  1:867197/1‑222 (MQ=255)                                                                                                                                                                                                                              aTCCCGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACATCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGATGac             <  1:66367/251‑1 (MQ=255)                                                                                                                                                                                                                                cccGAAAGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCAc                                                                                                                                           >  1:572859/1‑123 (MQ=255)                                                                                                                                                                                                                                    aaaGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCCTTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGATGACCAccc        <  1:127412/250‑1 (MQ=255)                                                                                                                                                                                                                                    aaaGGCGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGATGACCAccc        <  1:310503/250‑1 (MQ=255)                                                                                                                                                                                                                                         cGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGATGACCACCCTAAGc   <  1:583196/250‑1 (MQ=255)                                                                                                                                                                                                                                         cGACAGCCCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGATGACCACCCTAAGCa  <  1:699478/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                    AAACCTGGAGCGATAAGGAATTGTACTGTCTGCGGGCGTCCGTGGGTGCGCCGCCGGATGTGTTAGGTCCGCAAGGACAAAACTGGGGCTTAACGCCGATGAACCCGCACATTCTGAAACAACGCGCTTATCAGCCGTTTATCGACTTGGTGCGCGCCAATATGAATCATTGCGGTGCATTGCGTATCGATCACATTATGTCGTTATTGCGTTTATGGTGGATCCCGAAAGGCGACAGCTCGGTCAACGGCGCCTATGTGCGTTATCCGGTCGATGATTTAATCGCCATTCTCGCCCTTGAAAGCCAACGTCGTCGCTGCGTGATTATCGGCGAAGATTTAGGCACCGTGCCGAAAGAAATCGTCAGCAAACTCAAAAATGCCGGCATTTTGTCTTACAAAATCTTCTATTTTGAATTTGATAAACAAGGACAAAGTCGTGACCTGCGGGACTATCCGTACCAAGCGATGACCACCCTAAGCA  >  1/1376487‑1376969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑