Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,376,239	T→C	intergenic (+216659/+44698)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,376,239	0	T	C	100.0%	211.7 / NA	77	intergenic (+216659/+44698)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/47);  total (30/47)

AATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCATGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACCGCGACTTGGCGGTAGGCGTCAC  >  1/1375992‑1376472                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          aaTTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGcc                                                                                                                                                                                                                                        <  1:254357/251‑1 (MQ=255) aaTTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGcc                                                                                                                                                                                                                                        <  1:897636/251‑1 (MQ=255)   tttCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCa                                                                                                                                                                                                                                      >  1:12479/1‑251 (MQ=255)         caaAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAg                                                                                                                                                                                                                                <  1:767699/251‑1 (MQ=255)          aaaGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAgc                                                                                                                                                                                                                               <  1:358442/251‑1 (MQ=255)             gcgcAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAgcgc                                                                                                                                                                                                                             >  1:648419/1‑250 (MQ=255)              cgcAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGcc                                                                                                                                                                                                                            <  1:618918/250‑1 (MQ=255)                    ggCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGatat                                                                                                                                                                                                                      >  1:631256/1‑250 (MQ=255)                     gcgcAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGatat                                                                                                                                                                                                                      <  1:88901/249‑1 (MQ=255)                     gcgcAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGatat                                                                                                                                                                                                                      <  1:822725/249‑1 (MQ=255)                     gcgcAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATcg                                                                                                                                                                                                                    >  1:554662/1‑251 (MQ=255)                     gcgcAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTGACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGatat                                                                                                                                                                                                                      <  1:787356/249‑1 (MQ=255)                               ggTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATgg                                                                                                                                                                                                          >  1:645475/1‑251 (MQ=255)                               ggTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATgg                                                                                                                                                                                                          >  1:423056/1‑251 (MQ=255)                                gTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATggg                                                                                                                                                                                                         >  1:881894/1‑251 (MQ=255)                                gTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATggg                                                                                                                                                                                                         >  1:640142/1‑251 (MQ=255)                                  ttAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGt                                                                                                                                                                                                       <  1:465490/251‑1 (MQ=255)                                      ttCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTggg                                                                                                                                                                                                   <  1:270392/251‑1 (MQ=255)                                       tCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGa                                                                                                                                                                                                  <  1:833896/251‑1 (MQ=255)                                              aaGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGACGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTg                                                                                                                                                                                            <  1:460242/250‑1 (MQ=255)                                                                            ccgccgAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAg                                                                                                                                                             >  1:809473/1‑251 (MQ=255)                                                                               ccgAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGcc                                                                                                                                                                                                                                        <  1:87696/173‑1 (MQ=255)                                                                                          gattACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTc                                                                                                                                                                 >  1:351936/1‑233 (MQ=255)                                                                                          gattACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAAtt                                                                                                                                               >  1:4490/1‑251 (MQ=255)                                                                                           attACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTc                                                                                                                                              <  1:355938/251‑1 (MQ=255)                                                                                              aCGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTccg                                                                                                                                            >  1:559080/1‑250 (MQ=255)                                                                                              aCGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTccg                                                                                                                                            >  1:166125/1‑250 (MQ=255)                                                                                                  cGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTc                                                                                                                                       <  1:665059/251‑1 (MQ=255)                                                                                                    aGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCaa                                                                                                                                     >  1:457419/1‑251 (MQ=255)                                                                                                         aTGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTTCAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATgg                                                                                                                                <  1:664868/251‑1 (MQ=255)                                                                                                         aTGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATgg                                                                                                                                <  1:756029/251‑1 (MQ=255)                                                                                                         aTGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATgg                                                                                                                                <  1:672176/251‑1 (MQ=255)                                                                                                             cGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGa                                                                                                                             <  1:593651/250‑1 (MQ=255)                                                                                                             cGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAg                                                                                                                            >  1:126574/1‑251 (MQ=255)                                                                                                                tGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGaa                                                                                                                         <  1:499564/251‑1 (MQ=255)                                                                                                                  aaaTTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGaaa                                                                                                                        <  1:82571/250‑1 (MQ=255)                                                                                                                    aTTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCg                                                                                                                     >  1:637868/1‑251 (MQ=255)                                                                                                                                gCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATcgc                                                                                                                                                                                                                   <  1:754717/145‑1 (MQ=255)                                                                                                                                gCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGc                                                                                                           >  1:814323/1‑249 (MQ=255)                                                                                                                                gCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGc                                                                                                           >  1:133365/1‑249 (MQ=255)                                                                                                                                   ttttGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGt                                                                                                      >  1:119538/1‑251 (MQ=255)                                                                                                                                         ctttgccGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAAt                                                                                                <  1:263501/251‑1 (MQ=255)                                                                                                                                           ttgccGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTc                                                                                              <  1:397727/251‑1 (MQ=255)                                                                                                                                                  aTTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGa                                                                                                                          <  1:721381/216‑1 (MQ=255)                                                                                                                                                           aaaTCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCaacaac                                                                              <  1:764573/251‑1 (MQ=255)                                                                                                                                                                 gACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCg                                                                        >  1:305225/1‑251 (MQ=255)                                                                                                                                                                     gCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAgg                                                                                                                                                            >  1:294641/1‑163 (MQ=255)                                                                                                                                                                     gCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATgg                                                                                                                                >  1:419797/1‑191 (MQ=255)                                                                                                                                                                      cACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAgg                                                                                                                                                            >  1:826838/1‑162 (MQ=255)                                                                                                                                                                      cACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGc                                                                   <  1:816854/251‑1 (MQ=255)                                                                                                                                                                                  aaGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAGGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:320344/251‑1 (MQ=255)                                                                                                                                                                                  aaGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:873120/251‑1 (MQ=255)                                                                                                                                                                                  aaGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:339715/251‑1 (MQ=255)                                                                                                                                                                                     cTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTaa                                                     <  1:466419/250‑1 (MQ=255)                                                                                                                                                                                       tttGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAgc                                                  <  1:735271/251‑1 (MQ=255)                                                                                                                                                                                                 tCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATg                                        <  1:761793/251‑1 (MQ=255)                                                                                                                                                                                                 tCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATg                                        <  1:99344/251‑1 (MQ=255)                                                                                                                                                                                                    tGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTc                                                                                              >  1:113130/1‑194 (MQ=255)                                                                                                                                                                                                        cAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCg                                 >  1:542835/1‑251 (MQ=255)                                                                                                                                                                                                          aCGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGc                               <  1:689132/251‑1 (MQ=255)                                                                                                                                                                                                               ggCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATgg                                                                                                                                <  1:884284/149‑1 (MQ=255)                                                                                                                                                                                                                 cGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTAc                         <  1:888631/250‑1 (MQ=255)                                                                                                                                                                                                                   aaaGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACcg                       >  1:169105/1‑250 (MQ=255)                                                                                                                                                                                                                     aGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:557357/216‑1 (MQ=255)                                                                                                                                                                                                                     aGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:322447/216‑1 (MQ=255)                                                                                                                                                                                                                      gTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:100491/215‑1 (MQ=255)                                                                                                                                                                                                                      gTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACCGCGa                    >  1:797487/1‑250 (MQ=255)                                                                                                                                                                                                                      gTTTACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACCGCGa                    >  1:407463/1‑250 (MQ=255)                                                                                                                                                                                                                       tttACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTc                                                                                              <  1:829686/175‑1 (MQ=255)                                                                                                                                                                                                                       tttACAGGTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACCGCGAc                   <  1:193462/250‑1 (MQ=255)                                                                                                                                                                                                                             ggTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:208763/208‑1 (MQ=255)                                                                                                                                                                                                                             ggTGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGt                                                       <  1:483725/208‑1 (MQ=255)                                                                                                                                                                                                                               tGCAAGCCACTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACCGCGACTTGGCGGt           <  1:414954/250‑1 (MQ=255)                                                                                                                                                                                                                                        cTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATgggg                                                                                                                                                                                                        >  1:149030/1‑52 (MQ=255)                                                                                                                                                                                                                                        cTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAAt                                                                                                <  1:842328/156‑1 (MQ=255)                                                                                                                                                                                                                                        cTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTc                                                                                              <  1:272858/158‑1 (MQ=255)                                                                                                                                                                                                                                        cTTTTGATGCGCTGCACGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACCGCGACTTGGCGGTAGGCGTCAc  >  1:159685/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          AATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATTTAAAGAAAATCCGACCGCACTTTCCCAACAAGCTTTTGCCGATTTCGTGCGCAACGGTGGCGAAAGTTTACAGGTGCAAGCCACTTTTGATGCGCTGCATGCCCATTTAAGCGCCCGATATCGCGAGCAATGGGGTTGGGATTTCTGGGCGCCGGAATTCCAAGATTATCATTCCGAGGTCGTCGCGCAATTCCGCCGTCAATATGGTGAGGAAATCGAATTTTATGCCTGGTTGCAATTCTGCGCCGACCAACAACTTGCCGAATGCGATGCCTTATGTAAAGCGCAACAAATGACCATCGGCATGTACCGCGACTTGGCGGTAGGCGTCAC  >  1/1375992‑1376472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑