Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,375,896	C→T	intergenic (+216316/+45041)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,375,896	0	C	T	100.0%	206.7 / NA	77	intergenic (+216316/+45041)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/38);  total (39/38)

TCACGATCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGCCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATT  >  1/1375654‑1376139                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    tCACGATCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGTGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTc                                                                                                                                                                                                                                             <  1:310080/251‑1 (MQ=255)  cACGATCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTcc                                                                                                                                                                                                                                            <  1:783896/251‑1 (MQ=255)    cGATCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCgg                                                                                                                                                                                                                                          >  1:135488/1‑251 (MQ=255)      aTCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCggg                                                                                                                                                                                                                                         <  1:666092/250‑1 (MQ=255)      aTCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCggg                                                                                                                                                                                                                                         >  1:858952/1‑250 (MQ=255)      aTCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATA‑CCTGAAATCCGAGCGCAACTTGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCa                                                                                                                                                                                                                                       >  1:119635/1‑251 (MQ=255)           gtgtTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCCAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATc                                                                                                                                                                                                                                    >  1:426108/1‑250 (MQ=255)           gtgtTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATc                                                                                                                                                                                                                                    >  1:511027/1‑250 (MQ=255)                     gTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAAt                                                                                                                                                                                                                                     <  1:265331/239‑1 (MQ=255)                     gTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGc                                                                                                                                                                                                                          >  1:463163/1‑250 (MQ=255)                         aaaaGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGcc                                                                                                                                                                                                                     <  1:470366/251‑1 (MQ=255)                            agaagaagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGt                                                                                                                                                                                                                  >  1:867151/1‑251 (MQ=255)                             gaagaagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGcc                                                                                                                                                                                                                         <  1:732941/243‑1 (MQ=255)                              aagaagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGtat                                                                                                                                                                                                                <  1:345868/251‑1 (MQ=255)                                gaagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATgg                                                                                                                                                                                                                             >  1:800415/1‑236 (MQ=255)                                 aagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAg                                                                                                                                                                                                              <  1:577983/250‑1 (MQ=255)                                 aagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAg                                                                                                                                                                                                              <  1:770338/250‑1 (MQ=255)                                 aagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAg                                                                                                                                                                                                              <  1:44755/250‑1 (MQ=255)                                 aagTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAg                                                                                                                                                                                                              >  1:369298/1‑250 (MQ=255)                                   gTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGcc                                                                                                                                                                                                            >  1:608155/1‑250 (MQ=255)                                    tACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTGTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGccc                                                                                                                                                                                                           <  1:197383/250‑1 (MQ=255)                                    tACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGCTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGCGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGcc                                                                                                                                                                                                            <  1:438478/249‑1 (MQ=255)                                        ttGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCAt                                                                                                                                                                                                      <  1:374502/251‑1 (MQ=255)                                              ccTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGcc                                                                                                                                                                                                                     <  1:786073/230‑1 (MQ=255)                                                                gCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCAttt                                                                                                                                                                              >  1:140659/1‑251 (MQ=255)                                                                 cGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAAcatcat                                                                                                                                                                                <  1:520772/248‑1 (MQ=255)                                                                   ttgttATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACa                                                                                                                                                                           <  1:145370/251‑1 (MQ=255)                                                                    tgttATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACAt                                                                                                                                                                          <  1:325229/251‑1 (MQ=255)                                                                     gttATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCttt                                                                                                                                                                                                                                              >  1:761507/1‑182 (MQ=255)                                                                     gttATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACAt                                                                                                                                                                          >  1:441224/1‑250 (MQ=255)                                                                           aaCCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACAt                                                                                                                                                                    <  1:565725/250‑1 (MQ=255)                                                                           aaCCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACAt                                                                                                                                                                    <  1:440774/250‑1 (MQ=255)                                                                           aaCCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATa                                                                                                                                                                   >  1:898677/1‑251 (MQ=255)                                                                              cGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGAt                                                                                                                                                                <  1:582413/251‑1 (MQ=255)                                                                                gAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATc                                                                                                                                                               <  1:57317/250‑1 (MQ=255)                                                                                gAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCa                                                                                                                                                              >  1:624725/1‑251 (MQ=255)                                                                                                        ctctGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAgcg                                                                                                                                       >  1:498470/1‑250 (MQ=255)                                                                                                              ggCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAgg                                                                                                                                 <  1:882596/250‑1 (MQ=255)                                                                                                                  gTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCaa                                                                                                                            >  1:408962/1‑251 (MQ=255)                                                                                                                  gTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCaa                                                                                                                            >  1:58774/1‑251 (MQ=255)                                                                                                                            gCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGtat                                                                                                                                                                                                                <  1:386162/157‑1 (MQ=255)                                                                                                                            gCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTa                                                                                                                  >  1:861473/1‑251 (MQ=255)                                                                                                                            gCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTa                                                                                                                  >  1:377147/1‑251 (MQ=255)                                                                                                                            gCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTa                                                                                                                  >  1:586194/1‑251 (MQ=255)                                                                                                                            gCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTa                                                                                                                  >  1:32438/1‑251 (MQ=255)                                                                                                                            gCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGGGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAg                                                                                                                                                                                                              >  1:347411/1‑159 (MQ=255)                                                                                                                                tataCCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTc                                                                                                              <  1:852023/251‑1 (MQ=255)                                                                                                                                   aCCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTcgg                                                                                                            >  1:759768/1‑250 (MQ=255)                                                                                                                                    cccTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCAttt                                                                                                                                                                              >  1:572110/1‑183 (MQ=255)                                                                                                                                    cccTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAgg                                                                                                                                 >  1:267803/1‑228 (MQ=255)                                                                                                                                     ccTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTcggcgg                                                                                                         <  1:645310/251‑1 (MQ=255)                                                                                                                                             ccGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAg                                                                                                 <  1:180165/251‑1 (MQ=255)                                                                                                                                             ccGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAg                                                                                                 <  1:241496/251‑1 (MQ=255)                                                                                                                                                    cAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAAc                                                                                                                                                            >  1:530187/1‑185 (MQ=255)                                                                                                                                                    cAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAAc                                                                                                                                                            >  1:6229/1‑185 (MQ=255)                                                                                                                                                    cAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCaa                                                                                                                            >  1:869988/1‑217 (MQ=255)                                                                                                                                                    cAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAAt                                                                                          >  1:20653/1‑251 (MQ=255)                                                                                                                                                      aCTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGAt                                                                                                                                                                >  1:684780/1‑179 (MQ=255)                                                                                                                                                      aCTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTg                                                                                        >  1:841401/1‑251 (MQ=255)                                                                                                                                                                  gCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTAcg                                                                             <  1:502792/250‑1 (MQ=255)                                                                                                                                                                                       aaTTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTa                                                        >  1:769449/1‑250 (MQ=255)                                                                                                                                                                                       aaTTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTa                                                        <  1:187279/250‑1 (MQ=255)                                                                                                                                                                                         tttATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGc                                                     <  1:235543/251‑1 (MQ=255)                                                                                                                                                                                               cgcgGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGt                                               <  1:451215/251‑1 (MQ=255)                                                                                                                                                                                                   ggCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGcc                                                    >  1:177350/1‑242 (MQ=255)                                                                                                                                                                                                   ggCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATg                                           >  1:817187/1‑251 (MQ=255)                                                                                                                                                                                                     cTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGa                                                                                                                                                                 <  1:872309/131‑1 (MQ=255)                                                                                                                                                                                                     cTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATgg                                          <  1:121307/250‑1 (MQ=255)                                                                                                                                                                                                        gcgcCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATc                                                                                                                                                                         <  1:565542/120‑1 (MQ=255)                                                                                                                                                                                                                     cAGATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAggg                          <  1:723973/250‑1 (MQ=255)                                                                                                                                                                                                                       gATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTc                                                                                                              >  1:598801/1‑164 (MQ=255)                                                                                                                                                                                                                       gATTTTTTAGGCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTG‑CAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGgattgatt                                                         <  1:406720/216‑1 (MQ=255)                                                                                                                                                                                                                                ggCTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATtttg               >  1:307242/1‑250 (MQ=255)                                                                                                                                                                                                                                  cTTAAACCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATtttgcc             <  1:121130/250‑1 (MQ=255)                                                                                                                                                                                                                                       aCCCGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTAcgcg                                                                           >  1:352946/1‑183 (MQ=255)                                                                                                                                                                                                                                        cccGATTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCg      <  1:705270/251‑1 (MQ=255)                                                                                                                                                                                                                                            aTTCATGTCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAAtt  >  1:408064/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    TCACGATCTGGTGTTGCACGGTGGAAAAGAAGAAGTACATTGCCGCCTTATCGTTGCGCCGCAGCGTTGTTATGAACCGGAAGCATTCGCCGAACAGAAAAAACTCTGGGGCAGTTTTATTCAGCTTTATACCCTGAAATCCGAGCGCAACTGGGGCGTGGGCGATTTCGGTGATTTGCAAGAATTTATTCGCGGGCTTGCGCCGTATCAGGCAGATTTTTTAGGCTTAAACCCGATTCATGCCCTCTTTCCGGGCAATCCCGATGGTGCCAGCCCGTATAGCCCGTCATCGCGCAAATGGCTGAACATCATTTACATCAACATAGATCAACTGCCGGAATTTCAACAAAGCGCAGAGGCGCAACGCTGGTTTAATTCGGCGGAAGTACAGGAAAAATTGAACGCCTTACGCGCCGCCGAATGGATTGATTACGCCGAGGTGATGGCGTTGAAATTGAAGGGGCTGCATTTTGCCTTTGCCGAATT  >  1/1375654‑1376139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑