Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,368,449	A→G	intergenic (+208869/+52488)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,368,449	0	A	G	100.0%	190.9 / NA	69	intergenic (+208869/+52488)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/43);  total (26/43)

GAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCACCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACACCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATCTGTCAAAAATTCTTATACAATTTCTGTACAAAAAACAGACATTACAAATTTTTAACAAATAATACGA  >  1/1368206‑1368694                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCcttct                                                                                                                                                                                                                                                 <  1:418839/250‑1 (MQ=255)     gTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACg                                                                                                                                                                                                                                            >  1:341270/1‑251 (MQ=255)       aGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGaa                                                                                                                                                                                                                                          >  1:363004/1‑251 (MQ=255)         aTAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAAtt                                                                                                                                                                                                                                        >  1:722887/1‑251 (MQ=255)                         aTCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATtg                                                                                                                                                                                                                           >  1:287338/1‑248 (MQ=255)                           cAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGcc                                                                                                                                                                                                                      <  1:419474/251‑1 (MQ=255)                               gTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCcttct                                                                                                                                                                                                                                                 >  1:211394/1‑220 (MQ=255)                                           aCCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGtt                                                                                                                                                                                                       <  1:678029/250‑1 (MQ=255)                                                   acacCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGa                                                                                                                                                                                              >  1:807548/1‑251 (MQ=255)                                                          catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:326979/251‑1 (MQ=255)                                                          catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:277525/251‑1 (MQ=255)                                                          catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:486425/251‑1 (MQ=255)                                                          catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:85979/251‑1 (MQ=255)                                                          catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:501085/251‑1 (MQ=255)                                                               caGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACGCTCTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       >  1:592162/1‑248 (MQ=255)                                                               caGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGttt                                                                                                                                                                                                      >  1:274639/1‑231 (MQ=255)                                                                 gCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:88146/244‑1 (MQ=255)                                                                            gACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCGGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAAtt                                                                                                                                                                        >  1:379861/1‑248 (MQ=255)                                                                            gACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:176303/233‑1 (MQ=255)                                                                                          tACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTcc                                                                                                                                                       <  1:480404/251‑1 (MQ=255)                                                                                                       gTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGaa                                                                                                                                                                                                                                          <  1:607771/155‑1 (MQ=255)                                                                                                               ttCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                                                                                                  <  1:189823/251‑1 (MQ=255)                                                                                                               ttCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                                                                                                  <  1:579251/251‑1 (MQ=255)                                                                                                                tCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGt                                                                                                                                 <  1:218079/251‑1 (MQ=255)                                                                                                                     gATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGt                                                                                                                             <  1:32087/250‑1 (MQ=255)                                                                                                                               cTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTTCGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACt                                                                                                                   <  1:48223/250‑1 (MQ=255)                                                                                                                                 gTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                                                                                                  >  1:234870/1‑233 (MQ=255)                                                                                                                                 gTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAAc                                                                                                                        <  1:140505/243‑1 (MQ=255)                                                                                                                                    cAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                                                                                       <  1:457785/177‑1 (MQ=255)                                                                                                                                       aaaCCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAg                                                                                                            <  1:315970/249‑1 (MQ=255)                                                                                                                                             ggATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGCAACGCCGCCACTACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATc                                                                                                                                                                                                 >  1:770099/1‑158 (MQ=255)                                                                                                                                              gATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACg                                                                                                                                                                                        <  1:487364/166‑1 (MQ=255)                                                                                                                                               aTCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAaac                                                                                                   <  1:722019/250‑1 (MQ=255)                                                                                                                                               aTCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAaac                                                                                                   <  1:626854/250‑1 (MQ=255)                                                                                                                                               aTCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAaac                                                                                                   <  1:200385/250‑1 (MQ=255)                                                                                                                                                  gTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATc                                                                                                                                                                                            >  1:87504/1‑158 (MQ=255)                                                                                                                                                  gTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGt                                                                                                                             >  1:248798/1‑221 (MQ=255)                                                                                                                                                   tATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGa                                                                                              <  1:838102/251‑1 (MQ=255)                                                                                                                                                       cACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCt                                                                                                                                                 <  1:798481/196‑1 (MQ=255)                                                                                                                                                       cACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                                                                                                  >  1:373740/1‑211 (MQ=255)                                                                                                                                                       cACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGt                                                                                                                                 >  1:719212/1‑212 (MQ=255)                                                                                                                                                            aaTCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATc                                                                                                                                                  <  1:515712/190‑1 (MQ=255)                                                                                                                                                               cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACAt                                                                                                                                                                 <  1:414423/172‑1 (MQ=255)                                                                                                                                                               cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACttt                                                                                                                 <  1:431504/220‑1 (MQ=255)                                                                                                                                                               cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTaa                                                                                  >  1:599485/1‑251 (MQ=255)                                                                                                                                                               cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTaa                                                                                  >  1:756715/1‑251 (MQ=255)                                                                                                                                                               cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACCTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAg                                                                                                            <  1:438697/225‑1 (MQ=255)                                                                                                                                                                 gtagtaATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGc                                                                                                                                                              <  1:862052/173‑1 (MQ=255)                                                                                                                                                                 gtagtaATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                                                                                                  >  1:883489/1‑201 (MQ=255)                                                                                                                                                                     taATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAata                                                                              <  1:479286/249‑1 (MQ=255)                                                                                                                                                                            tGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCGATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATc                                                                     <  1:37126/251‑1 (MQ=255)                                                                                                                                                                            tGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATc                                                                     <  1:156557/251‑1 (MQ=255)                                                                                                                                                                                                  aTACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAAcc                                                                                                                       <  1:501426/179‑1 (MQ=255)                                                                                                                                                                                                             ctgcATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAAc                                                                                                                                                                            <  1:753274/115‑1 (MQ=255)                                                                                                                                                                                                             ctgcATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAAcc                                                                                                                       <  1:135104/168‑1 (MQ=255)                                                                                                                                                                                                                   cACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCt                                                                                                                                                 >  1:64413/1‑136 (MQ=255)                                                                                                                                                                                                                     cTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATCTGTCAAAAATTCTTATACAATTTCTGTACAAAAAACAGACa                            <  1:414171/251‑1 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATc                                                                                                                                                                                                 <  1:249897/78‑1 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAAcc                                                                                                                       >  1:763518/1‑152 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACttt                                                                                                                 >  1:345105/1‑158 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAAc                                                                                     >  1:126493/1‑186 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAatat                                                                             <  1:35225/194‑1 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATCTGTCAAAAATTCTTATACAATTTCTg                                           <  1:114645/228‑1 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATCTGTCAAAAATTCTTATACAATTTCTGTAc                                        >  1:362286/1‑231 (MQ=255)                                                                                                                                                                                                                             ataatGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTACCATCCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGt                                                                           <  1:644458/196‑1 (MQ=255)                                                                                                                                                                                                                                  gAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGt                                                                                                                             >  1:130541/1‑141 (MQ=255)                                                                                                                                                                                                                                                cTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATCTGTCAAAAATTCTTATACAATTTCTGTACAAAAAACAGACATTACAAATTTTTAACAAATAATACg   >  1:674745/1‑251 (MQ=255)                                                                                                                                                                                                                                                 tCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATCTGTCAAAAATTCTTATACAATTTCTGTACAAAAAACAGACATTACAAATTTTTAACAAATAATACGa  <  1:345282/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAaac                                                                                                   >  1:121456/1‑153 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCACCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACACCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATCTGTCAAAAATTCTTATACAATTTCTGTACAAAAAACAGACATTACAAATTTTTAACAAATAATACGA  >  1/1368206‑1368694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑