Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,368,395	A→G	intergenic (+208815/+52542)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,368,395	0	A	G	100.0%	204.0 / NA	73	intergenic (+208815/+52542)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/46);  total (27/46)

CGGCTTTGAAGAAATGATTAAAACAAACTTCATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCACCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACACCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATC  >  1/1368154‑1368627                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           cGGCTTTGAAGAAATGATTAAAACAAACTTCATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACg                                                                                                                                                                                                                                    <  1:706013/250‑1 (MQ=255)   gCTTTGAAGAAATGATTAAAACAAACTTCATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAg                                                                                                                                                                                                                                  >  1:302023/1‑250 (MQ=255)               tGATTAAAACAAACTTCATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCAc                                                                                                                                                                                                                     <  1:833618/251‑1 (MQ=255)                            cTTCATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACt                                                                                                                                                                                                                    <  1:475202/239‑1 (MQ=255)                            cTTCATAAACCGTTGAAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGAt                                                                                                                                                                                                                >  1:888728/1‑243 (MQ=255)                               cATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAAc                                                                                                                                                                                                     <  1:847816/251‑1 (MQ=255)                               cATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAAc                                                                                                                                                                                                     <  1:721382/251‑1 (MQ=255)                               cATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAAc                                                                                                                                                                                                     <  1:365832/251‑1 (MQ=255)                                        ttGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAAc                                                                                                                                                                                                     >  1:341397/1‑242 (MQ=255)                                        ttGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAAc                                                                                                                                                                                                     <  1:485353/242‑1 (MQ=255)                                                 aTTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAacgc                                                                                                                                                                                   >  1:701427/1‑247 (MQ=255)                                                 aTTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAacgc                                                                                                                                                                                   <  1:364066/249‑3 (MQ=255)                                                 aTTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAacgc                                                                                                                                                                                   <  1:577145/249‑3 (MQ=255)                                                     gAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCcttct                                                                                                                                                                              <  1:418839/250‑1 (MQ=255)                                                         gTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCAt                                                                                                                                                                                                                        <  1:21827/206‑1 (MQ=255)                                                         gTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCa                                                                                                                                                                                                              >  1:826/1‑216 (MQ=255)                                                         gTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAAc                                                                                                                                                                                                     >  1:350028/1‑225 (MQ=255)                                                         gTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACg                                                                                                                                                                         >  1:341270/1‑251 (MQ=255)                                                           aGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGaa                                                                                                                                                                       >  1:363004/1‑251 (MQ=255)                                                             aTAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAAtt                                                                                                                                                                     >  1:722887/1‑251 (MQ=255)                                                                            aaTCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACtt                                                                                                                                                                                                                   >  1:130628/1‑192 (MQ=255)                                                                             aTCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATtg                                                                                                                                                        >  1:287338/1‑248 (MQ=255)                                                                               cAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGcc                                                                                                                                                   <  1:419474/251‑1 (MQ=255)                                                                                   gTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCAc                                                                                                                                                                                                                     <  1:272645/183‑1 (MQ=255)                                                                                   gTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAacgcc                                                                                                                                                                                  >  1:833032/1‑213 (MQ=255)                                                                                   gTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCcttct                                                                                                                                                                              >  1:211394/1‑220 (MQ=255)                                                                                        ttttCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAacgcc                                                                                                                                                                                  >  1:753687/1‑208 (MQ=255)                                                                                               aCCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGtt                                                                                                                                    <  1:678029/250‑1 (MQ=255)                                                                                                       acacCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGa                                                                                                                           >  1:807548/1‑251 (MQ=255)                                                                                                              catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:486425/251‑1 (MQ=255)                                                                                                              catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:501085/251‑1 (MQ=255)                                                                                                              catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:85979/251‑1 (MQ=255)                                                                                                              catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:326979/251‑1 (MQ=255)                                                                                                              catcaCAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:277525/251‑1 (MQ=255)                                                                                                                   caGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACGCTCTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    >  1:592162/1‑248 (MQ=255)                                                                                                                   caGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGttt                                                                                                                                   >  1:274639/1‑231 (MQ=255)                                                                                                                     gCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:88146/244‑1 (MQ=255)                                                                                                                                gACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCGGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAAtt                                                                                                     >  1:379861/1‑248 (MQ=255)                                                                                                                                gACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:176303/233‑1 (MQ=255)                                                                                                                                  cATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAAc                                                                                                                                                                                                     <  1:749425/152‑1 (MQ=255)                                                                                                                                              tACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTcc                                                                                    <  1:480404/251‑1 (MQ=255)                                                                                                                                                           gTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGaa                                                                                                                                                                       <  1:607771/155‑1 (MQ=255)                                                                                                                                                                   ttCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                               <  1:189823/251‑1 (MQ=255)                                                                                                                                                                   ttCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                               <  1:579251/251‑1 (MQ=255)                                                                                                                                                                    tCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGt                                                              <  1:218079/251‑1 (MQ=255)                                                                                                                                                                         gATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGt                                                          <  1:32087/250‑1 (MQ=255)                                                                                                                                                                                   cTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTTCGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACt                                                <  1:48223/250‑1 (MQ=255)                                                                                                                                                                                     gTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                               >  1:234870/1‑233 (MQ=255)                                                                                                                                                                                     gTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAAc                                                     <  1:140505/243‑1 (MQ=255)                                                                                                                                                                                        cAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGt                                                                                                                    <  1:457785/177‑1 (MQ=255)                                                                                                                                                                                           aaaCCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAg                                         <  1:315970/249‑1 (MQ=255)                                                                                                                                                                                                 ggATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGCAACGCCGCCACTACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATc                                                                                                                              >  1:770099/1‑158 (MQ=255)                                                                                                                                                                                                  gATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACg                                                                                                                     <  1:487364/166‑1 (MQ=255)                                                                                                                                                                                                   aTCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAaac                                <  1:722019/250‑1 (MQ=255)                                                                                                                                                                                                   aTCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAaac                                <  1:626854/250‑1 (MQ=255)                                                                                                                                                                                                   aTCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAaac                                <  1:200385/250‑1 (MQ=255)                                                                                                                                                                                                      gTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATc                                                                                                                         >  1:87504/1‑158 (MQ=255)                                                                                                                                                                                                      gTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGt                                                          >  1:248798/1‑221 (MQ=255)                                                                                                                                                                                                       tATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGa                           <  1:838102/251‑1 (MQ=255)                                                                                                                                                                                                           cACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCt                                                                              <  1:798481/196‑1 (MQ=255)                                                                                                                                                                                                           cACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                               >  1:373740/1‑211 (MQ=255)                                                                                                                                                                                                           cACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGt                                                              >  1:719212/1‑212 (MQ=255)                                                                                                                                                                                                                aaTCTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATc                                                                               <  1:515712/190‑1 (MQ=255)                                                                                                                                                                                                                   cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACAt                                                                                              <  1:414423/172‑1 (MQ=255)                                                                                                                                                                                                                   cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACttt                                              <  1:431504/220‑1 (MQ=255)                                                                                                                                                                                                                   cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTaa               >  1:599485/1‑251 (MQ=255)                                                                                                                                                                                                                   cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTaa               >  1:756715/1‑251 (MQ=255)                                                                                                                                                                                                                   cTGTAGTAATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACCTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAg                                         <  1:438697/225‑1 (MQ=255)                                                                                                                                                                                                                     gtagtaATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGc                                                                                           <  1:862052/173‑1 (MQ=255)                                                                                                                                                                                                                     gtagtaATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAg                                                               >  1:883489/1‑201 (MQ=255)                                                                                                                                                                                                                         taATCCCTGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAata           <  1:479286/249‑1 (MQ=255)                                                                                                                                                                                                                                tGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCGATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATc  <  1:37126/251‑1 (MQ=255)                                                                                                                                                                                                                                tGTCGGCAAAGAAACGCCGCCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACGCCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATc  <  1:156557/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           CGGCTTTGAAGAAATGATTAAAACAAACTTCATAAACCGTTGCAGCAGATTGGAAAGTAGATAAGTGACCGCCTAAATCAAGGTCTTTTTTCTGACCGCGCAACACCATCATCACAGCATTCCAGCGGACATAAGAACGAATACGACGCTCAATGTCTAAATTTCCCGGATACTCGGGCTGTTCAGAAACCGGGATCGTATTCACGTAATCTGTAGTAATCCCTGTCGGCAAAGAAACGCCACCAATACGAGCTTGCTGCATCACTTGATCAATAATGAACTGCGCACG‑‑CTCAACACCTTCTTCACGAATTAACGAATCAATTGCTGCCAACCAATCGTTAGTTTCGATCGGATCTACGTCATTTTTTAACAATTCTGACATAGCTTTTTCCTTATCTTTGTTAAGTTAGAAGTACGTTTAACCTACTTTTGTAGAAATTAAACAACGAATTGATAACTAAAATATGTTTAATC  >  1/1368154‑1368627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑