Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,362,656	G→A	intergenic (+203076/+58281)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,362,656	0	G	A	100.0%	211.0 / NA	79	intergenic (+203076/+58281)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/36);  total (43/36)

TACCTAGCATGACGATTTAATCAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAGCAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAATACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTCTTTATCGAAAATCAGTTTGGTCATAC  >  1/1362422‑1362902                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       tACCTAGCATGACGATTTAATCAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTttctt                                                                                                                                                                                                                                        >  1:263939/1‑251 (MQ=255)                   aaTCAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTaa                                                                                                                                                                                                                      >  1:716972/1‑251 (MQ=255)                   aaTCAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTaa                                                                                                                                                                                                                      >  1:621415/1‑251 (MQ=255)                    aTCAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAg                                                                                                                                                                                                                     >  1:193580/1‑251 (MQ=255)                      cAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTc                                                                                                                                                                                                                   <  1:463281/251‑1 (MQ=255)                      cAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTc                                                                                                                                                                                                                   <  1:613855/251‑1 (MQ=255)                            ttGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTgac                                                                                                                                                                                                             <  1:273168/251‑1 (MQ=255)                             tGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGt                                                                                                                                                                                                                <  1:45400/247‑1 (MQ=255)                              gCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTgacc                                                                                                                                                                                                            <  1:523212/250‑1 (MQ=255)                              gCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGA‑TTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGaatatgttacacccaaccgaa                                                                                                                                                                                                          >  1:832584/1‑231 (MQ=255)                                cccACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTgaccga                                                                                                                                                                                                          >  1:514363/1‑250 (MQ=255)                                      tGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCa                                                                                                                                                                                                   >  1:736272/1‑251 (MQ=255)                                      tGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCa                                                                                                                                                                                                   >  1:422079/1‑251 (MQ=255)                                         tAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAac                                                                                                                                                                                                <  1:19964/251‑1 (MQ=255)                                          aaaCCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTc                                                                                                                                                                                                                   <  1:742970/231‑1 (MQ=255)                                           aaCCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTCTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAacac                                                                                                                                                                                              <  1:378808/251‑1 (MQ=255)                                              ccAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTc                                                                                                                                                                                           <  1:284170/251‑1 (MQ=255)                                                  gTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTgac                                                                                                                                                                                                             >  1:693281/1‑229 (MQ=255)                                                  gTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGc                                                                                                                                                                                        >  1:624841/1‑250 (MQ=255)                                                        agaTAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGa                                                                                                                                                                                 >  1:900868/1‑251 (MQ=255)                                                         gaTAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCg                                                                                                                                                                                      <  1:613833/245‑1 (MQ=255)                                                            aaaTCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGa                                                                                                                                                                             >  1:355069/1‑251 (MQ=255)                                                            aaaTCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGa                                                                                                                                                                             >  1:63567/1‑251 (MQ=255)                                                               tCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTc                                                                                                                                                                                                                   >  1:524469/1‑210 (MQ=255)                                                                  cccATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTc                                                                                                                                                                                                                   >  1:395707/1‑207 (MQ=255)                                                                  cccATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCgg                                                                                                                                                                                                                 <  1:32378/209‑1 (MQ=255)                                                                  cccATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGCCCGCAAGACCGACAGAtt                                                                                                                                                                       >  1:32560/1‑251 (MQ=255)                                                                    cATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCg                                                                                                                                                                     <  1:898399/251‑1 (MQ=255)                                                                    cATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCg                                                                                                                                                                     >  1:625386/1‑251 (MQ=255)                                                                      ttAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCgtg                                                                                                                                                                   <  1:792954/251‑1 (MQ=255)                                                                            cATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTgacc                                                                                                                                                                                                            >  1:766781/1‑204 (MQ=255)                                                                            cATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGt                                                                                                                                                             >  1:18608/1‑251 (MQ=255)                                                                                 caGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGt                                                                                                                                                                                                                        >  1:691791/1‑187 (MQ=255)                                                                                 caGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGt                                                                                                                                                        >  1:895260/1‑251 (MQ=255)                                                                                       aCCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTgg                                                                                                                                                      <  1:781957/247‑1 (MQ=255)                                                                                        ccATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTg                                                                                                                                                 >  1:738238/1‑251 (MQ=255)                                                                                           ttGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGAt                                                                                                                                              <  1:240615/251‑1 (MQ=255)                                                                                                aaTCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCa                                                                                                                                         <  1:695981/251‑1 (MQ=255)                                                                                                        caaacaaaCTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCg                                                                                                                                    <  1:232867/248‑1 (MQ=255)                                                                                                                                  aTTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGAt                                                                                                                                              >  1:365919/1‑212 (MQ=255)                                                                                                                                  aTTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGAt                                                                                                       >  1:259041/1‑251 (MQ=255)                                                                                                                                            cACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCg                                                                                                                                    >  1:771676/1‑212 (MQ=255)                                                                                                                                            cACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCa                                                                                             >  1:396080/1‑251 (MQ=255)                                                                                                                                                ttATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAAttt                                                                                         <  1:31992/251‑1 (MQ=255)                                                                                                                                                           aTAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAAt                                                                              >  1:478265/1‑251 (MQ=255)                                                                                                                                                             aaaGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTc                                                                            <  1:205902/251‑1 (MQ=255)                                                                                                                                                               aGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCa                                                                           >  1:295832/1‑250 (MQ=255)                                                                                                                                                                gTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCAc                                                                          >  1:650491/1‑250 (MQ=255)                                                                                                                                                                 tGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCAcc                                                                         <  1:549228/250‑1 (MQ=255)                                                                                                                                                                            ggTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAAc                                                                                 <  1:722763/231‑1 (MQ=255)                                                                                                                                                                                 aCTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTc                                                                                                                                          <  1:850866/169‑1 (MQ=255)                                                                                                                                                                                 aCTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCcgacga                                                        >  1:73454/1‑251 (MQ=255)                                                                                                                                                                                    gAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGa                                                                                                                                   >  1:325633/1‑173 (MQ=255)                                                                                                                                                                                    gAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAAttt                                                                                         <  1:302576/215‑1 (MQ=255)                                                                                                                                                                                    gAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATgg                                                     >  1:97568/1‑251 (MQ=255)                                                                                                                                                                                    gAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATgg                                                     >  1:469189/1‑251 (MQ=255)                                                                                                                                                                                         ataCTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCg                                                                                                                                    >  1:524713/1‑167 (MQ=255)                                                                                                                                                                                         ataCTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAAttt                                                                                         >  1:689536/1‑210 (MQ=255)                                                                                                                                                                                           aCTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGt                                                                                                                                <  1:160049/169‑1 (MQ=255)                                                                                                                                                                                            cTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAac                                                                                                                                                                                                >  1:85508/1‑104 (MQ=255)                                                                                                                                                                                                cAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTgac                                                                                                                                                                                                             <  1:597271/87‑1 (MQ=255)                                                                                                                                                                                                cAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCcgc                                                                                                                         <  1:752181/171‑1 (MQ=255)                                                                                                                                                                                                cAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTa                                         >  1:284076/1‑251 (MQ=255)                                                                                                                                                                                                cAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTa                                         >  1:155714/1‑251 (MQ=255)                                                                                                                                                                                                 aaTAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTa                                         >  1:729375/1‑250 (MQ=255)                                                                                                                                                                                                 aaTAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTATCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACGCTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTACCGCGTCACAACCGATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTAc                                        <  1:36335/251‑1 (MQ=255)                                                                                                                                                                                                  aTAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGAt                                                                                                                                                                                                                             <  1:74506/69‑1 (MQ=255)                                                                                                                                                                                                  aTAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCAc                                                                          <  1:74364/216‑1 (MQ=255)                                                                                                                                                                                                  aTAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTAcg                                       >  1:674239/1‑251 (MQ=255)                                                                                                                                                                                                  aTAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTAcg                                       >  1:585864/1‑251 (MQ=255)                                                                                                                                                                                                         ccTTCCCAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATg                                  <  1:893705/249‑1 (MQ=255)                                                                                                                                                                                                             cccAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTc                            <  1:15888/251‑1 (MQ=255)                                                                                                                                                                                                             cccAACGGAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTc                            <  1:852166/251‑1 (MQ=255)                                                                                                                                                                                                                    gAAAGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTCTTTATCg                     >  1:273692/1‑251 (MQ=255)                                                                                                                                                                                                                     aaaGGCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTCTTTATCg                     <  1:855221/250‑1 (MQ=255)                                                                                                                                                                                                                        ggCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGt                                                                                                                                                             <  1:786214/111‑1 (MQ=255)                                                                                                                                                                                                                        ggCTTTTATTTTTAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTCTTTATCGaaaa                 >  1:331080/1‑251 (MQ=255)                                                                                                                                                                                                                                    tAATGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGc                                                              <  1:468679/194‑1 (MQ=255)                                                                                                                                                                                                                                       tGAAACAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAAGACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTCTTTATCGAAAATCAGTTTGGTCATAc  <  1:570221/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       TACCTAGCATGACGATTTAATCAAACGTTGCCCCACGTGGTAAACCCAAGTATTGAGATAAATCACCCATTAACCCATCACAGCTGACCATTGCCAATCGCAGCAAACAAACTGCAGATTTTTCACTCAATTCCGACCGCACTTTATTTGTTTGATAAAGTGATTTTCATCGGTTAACTGAATTATACTTACAATAAAAGCCTTCCCAACGGAAAGGCTTTTATTTTTAATGAAGCAAATTATTTTTTCTTCGCTTTTGGATTAGGTAAGTCGGTGACCGACCCCTCAAACACTTCGGCCGCAAGACCGACAGATTCGTGTAAAGTCGGGTGGGCGTGGATAGTCAATGCGATGTCTTCCGCGTCACAACCCATTTCGATGGCAATACCAATTTCGCCTAACAATTCACCGGCATTGGTGCCGACGATGGCGCCGCCAAGTACGCGATGCGTGTCTTTATCGAAAATCAGTTTGGTCATAC  >  1/1362422‑1362902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑