Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,357,857	T→C	intergenic (+198277/+63080)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,357,857	0	T	C	100.0%	89.3 / NA	35	intergenic (+198277/+63080)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

CACGGGCTAACACTTTTTGTAATTTTTCGCCTTCTGCCGCGGAGGTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATTACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGACCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATCCGGCACGCGAATGCCGATGGTTTTGCGCTTGG  >  1/1357613‑1358098                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  cACGGGCTAACACTTTTTGTAATTTTTCGCCTTCTGCCGCGGAGGTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCgg                                                                                                                                                                                                                                              <  1:546888/250‑1 (MQ=255)             cTTTTTGTAATTTTTCGCCTTCTGCCGCGGAGGTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGtt                                                                                                                                                                                                                                 <  1:76054/251‑1 (MQ=255)                      aTTTTTCGCCTTCTGCCGCGGAGGTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATccacc                                                                                                                                                                                                                        <  1:854449/251‑1 (MQ=255)                      aTTTTTCGCCTTCTGCCGCGGAGGTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATccacc                                                                                                                                                                                                                        <  1:319707/251‑1 (MQ=255)                          ttCGCCTTCTGCCGCGGAGGTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCg                                                                                                                                                                                                                    <  1:428177/251‑1 (MQ=255)                                            ggTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCa                                                                                                                                                                                                  >  1:883373/1‑251 (MQ=255)                                              ttttGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTAGCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCaaa                                                                                                                                                                                                <  1:65852/251‑1 (MQ=255)                                                           gctgATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGaa                                                                                                                                                                                   >  1:316439/1‑251 (MQ=255)                                                                                     cATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCg                                                                                                                                                                                                                    >  1:644144/1‑192 (MQ=255)                                                                                                 tGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCTTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTCCCCAAGCGTCTGTGTTAATTAAACTAAGTATCTAATCTAAATAAATGGCGTACCCGCGCCGCTGCCTTCCCGCAAGATCCCCGGCGTGTCTCCGGTTAAACCCCCCCCCGTTGTCGGTTCTTGCCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGAtc                                                                                                                                             <  1:257469/251‑1 (MQ=255)                                                                                                       acgaAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTc                                                                                                                                        >  1:605720/1‑250 (MQ=255)                                                                                                           aaggggCGGTGGATTTTGGGTGCGTTTTGTGGTGGGTTTCATAAATCCTGGTGCCGCTTTCCCAGGCGTGTGTGTAAATTAAACTAAATATCTAACCTAAAAAAATGGCGAAACCGCGCCGCTGCCTCCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCCCCACCGTTGTCGGTTCTGGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGat                                                                                                                                   <  1:165009/246‑2 (MQ=255)                                                                                                                  ggTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGaat                                                                                                                                                                                  >  1:703311/1‑197 (MQ=255)                                                                                                                             aGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGtt                                                                                                                 >  1:747495/1‑251 (MQ=255)                                                                                                                                            gggTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGaat                                                                                                                                                                                  >  1:241705/1‑171 (MQ=255)                                                                                                                                                 tCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGat                                                                                                                                   >  1:543909/1‑212 (MQ=255)                                                                                                                                                     aaaTCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAAGGGCGTACCCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCAAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGc                                                                                         <  1:96602/251‑1 (MQ=255)                                                                                                                                                       aTCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAg                                                                                       >  1:316963/1‑251 (MQ=255)                                                                                                                                                                tcgttttCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAAGGGCGTACCCGCGCCGCTGCCTTCGCGGAAGATCCCCGGCGTGTCTCCGTTTAAATCCCCCCCCGTTGTCGGTTTTTGTCCAAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAAt                                                                               <  1:490629/246‑1 (MQ=255)                                                                                                                                                                tCGCTTTCCCAAGCGTTTGTTTTAATTTAACTAAGTATCTAATCTAAATAAAGGGCGTACCCGCGCCGCTGCCTTCACGCAAGATCCCCGGCGTGTCTCCGTTTAAATCCCCCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAAt                                                                               <  1:520090/250‑1 (MQ=255)                                                                                                                                                                      tCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTACCCGCGCCGCTGCCTTCACGCAAGATCCCCGGCGTGTCTCCGGTTAAATCCACCCCCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATc                                                                              <  1:547100/245‑1 (MQ=255)                                                                                                                                                                               tCTGTGTTAATTTAACTAAGTATCAAATCTAAAAAAAGGGCGTACCCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAACCCCCCACCGTTGTCGGTTCTTGCCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGc                                                                <  1:46085/250‑1 (MQ=255)                                                                                                                                                                                       aaTTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATc                                                                                                                                                                         >  1:740719/1‑137 (MQ=255)                                                                                                                                                                                           taaatAAGTATATAATCAAAAAAAAGGGCGTACCCGCGCCGTTCCCTTCCCGCAAGATCACGGGCGTGTCTCCGGTTAAATCCCCCCCCGTTGTCGGTTCTTGTCCAAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATcc                                                   <  1:885454/247‑1 (MQ=255)                                                                                                                                                                                                  gTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCgg                                                                            >  1:328669/1‑219 (MQ=255)                                                                                                                                                                                                    atctaatctaAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAAtt                                          >  1:241046/1‑251 (MQ=255)                                                                                                                                                                                                        aatctaaATAAATGGCGTACCCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCCCCGTTGTGGTTTCTTGTCCAAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGat                                                                                                                                   <  1:347244/158‑2 (MQ=255)                                                                                                                                                                                                             aaataaatGGCGTAACCGCGCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATc                                  >  1:795371/1‑250 (MQ=255)                                                                                                                                                                                                                          acccGCGCCGCTGCCTTCACGCAAGATCCCCGGCGTGTCTCCGTTTAAATCCCCCCCCGTTGTCGTTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATCCGGCACGCGAATGc                    <  1:158997/249‑1 (MQ=255)                                                                                                                                                                                                                          acccGCCCCGCTGCCTTCACGCAAGATCACCGGCGTGTCTCGGGTAAAACCCCCCCCCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATCCGGCACGCGAATGc                    <  1:649777/249‑1 (MQ=255)                                                                                                                                                                                                                                      gCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATcc                                 >  1:449/1‑226 (MQ=255)                                                                                                                                                                                                                                      gCCTTCACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATCCGGCACGCGAATGCCGATGGTTTTg         >  1:730911/1‑250 (MQ=255)                                                                                                                                                                                                                                      gCCTTCACGAAAGATCGCGGGGGTGTCTCCGGTAAAATCCACCACTGTTGTCGTTTCTTGCCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAg                                                          <  1:153636/201‑1 (MQ=255)                                                                                                                                                                                                                                           cACGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAAt                                                                               >  1:824420/1‑175 (MQ=255)                                                                                                                                                                                                                                            aCGCAAGATCACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGATCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATCCGGCACGCGAATGCCGATGGTTTTGCGCTTgg  >  1:217570/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  CACGGGCTAACACTTTTTGTAATTTTTCGCCTTCTGCCGCGGAGGTTTTGCTTGCGCTGCTGATCGGACGCGGCTTCGCCGTCGCATTTTTATCTTTGTTCGACGAAAAGTGCGGTCGATTTTGAGTGCGTTTTGTTGTGGGTTTCATAAATCCTCGTGTCGCTTTCCCAAGCGTCTGTGTTAATTTAACTAAGTATCTAATCTAAATAAATGGCGTAACCGCGCCGCTGCCTTCACGCAAGATTACCGGCGTGTCTCCGGTTAAATCCACCACCGTTGTCGGTTCTTGTCCCAAATAACCGCCGTGAATAATTAAATCCACCTGATGTTCCAAACGATCACGGATCTCTTCCGGACCCGATTGCGTCAAATGTTCTTCATTCGGTAGCATCAACGAGCAGGATAAAATCGGTTCACCAAGGGCTTTTAGTAAATCCAACGCAATTTGGTTATCCGGCACGCGAATGCCGATGGTTTTGCGCTTGG  >  1/1357613‑1358098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑