Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,356,080	C→T	intergenic (+196500/+64857)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,356,080	0	C	T	100.0%	184.0 / NA	65	intergenic (+196500/+64857)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/29);  total (36/29)

CATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATCCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAATATTGAGCTTCCG  >  1/1355834‑1356316                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             caTTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCa                                                                                                                                                                                                                                          >  1:17422/1‑251 (MQ=255)   ttattGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCAt                                                                                                                                                                                                                                         >  1:784337/1‑250 (MQ=255)     attGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCgg                                                                                                                                                                                                                                      >  1:309893/1‑251 (MQ=255)                tACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATGACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAAt                                                                                                                                                                                                                           <  1:704764/251‑1 (MQ=255)                             ttCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGc                                                                                                                                                                                                               <  1:124335/250‑1 (MQ=255)                                        cACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCa                                                                                                                                                                                                   >  1:368232/1‑251 (MQ=255)                                            ggTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAatat                                                                                                                                                                                                                    <  1:516994/230‑1 (MQ=255)                                            ggTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGc                                                                                                                                                                                                               >  1:30838/1‑235 (MQ=255)                                            ggTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCAt                                                                                                                                                                                               >  1:175531/1‑251 (MQ=255)                                            ggTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCAt                                                                                                                                                                                               >  1:809845/1‑251 (MQ=255)                                                   tGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAgg                                                                                                                                                                                         <  1:767807/250‑1 (MQ=255)                                                   tGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAg                                                                                                                                                                                          <  1:11195/249‑1 (MQ=255)                                                                          aTCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCgg                                                                                                                                                                 >  1:190243/1‑251 (MQ=255)                                                                               acataTAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAg                                                                                                                                                            >  1:713026/1‑251 (MQ=255)                                                                                cataTAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAg                                                                                                                                                            >  1:832735/1‑250 (MQ=255)                                                                                cataTAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAg                                                                                                                                                            >  1:679583/1‑250 (MQ=255)                                                                                cataTAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCACATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAg                                                                                                                                                            >  1:432626/1‑250 (MQ=255)                                                                                  tataATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCATCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCg                                                                                                                                                         <  1:432972/251‑1 (MQ=255)                                                                                  tataATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCg                                                                                                                                                         >  1:847147/1‑251 (MQ=255)                                                                                      aTTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAg                                                                                                                                                     <  1:410957/251‑1 (MQ=255)                                                                                         aCGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGtat                                                                                                                                                  >  1:748083/1‑251 (MQ=255)                                                                                         aCGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGtat                                                                                                                                                  >  1:872423/1‑251 (MQ=255)                                                                                              ggAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTa                                                                                                                                             >  1:263175/1‑251 (MQ=255)                                                                                               gAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGt                                                                                                                                              <  1:468331/249‑1 (MQ=255)                                                                                               gAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTa                                                                                                                                             <  1:802160/250‑1 (MQ=255)                                                                                               gAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTa                                                                                                                                             <  1:294375/250‑1 (MQ=255)                                                                                                    cgcCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTaa                                                                                                                                        <  1:58386/250‑1 (MQ=255)                                                                                                        gtgtTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGat                                                                                                                                   >  1:864881/1‑251 (MQ=255)                                                                                                          gtTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATAc                                                                                                                                                                                                                         >  1:208223/1‑163 (MQ=255)                                                                                                          gtTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGc                                                                                                                                                                                        <  1:855822/196‑1 (MQ=255)                                                                                                                              aTGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGt                                                                                                             >  1:667865/1‑251 (MQ=255)                                                                                                                                       cgGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCt                                                                                                     <  1:290305/250‑1 (MQ=255)                                                                                                                                         gAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAg                                                                                                                                               >  1:687563/1‑206 (MQ=255)                                                                                                                                         gAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTa                                                                                                                                             >  1:650134/1‑208 (MQ=255)                                                                                                                                         gAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTca                                                                                                   >  1:813675/1‑250 (MQ=255)                                                                                                                                         gAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTca                                                                                                   >  1:692760/1‑250 (MQ=255)                                                                                                                                         gAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTca                                                                                                   >  1:733809/1‑250 (MQ=255)                                                                                                                                                           aaGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGgatgat                                                                                >  1:78988/1‑251 (MQ=255)                                                                                                                                                             gTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGgatgat                                                                                >  1:369263/1‑249 (MQ=255)                                                                                                                                                                      aaTCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTcaca                                                                     >  1:181440/1‑251 (MQ=255)                                                                                                                                                                           aCATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGAc                                                                <  1:553786/251‑1 (MQ=255)                                                                                                                                                                           aCATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGAc                                                                <  1:751240/251‑1 (MQ=255)                                                                                                                                                                                    ggCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGt                                                                                                                                              >  1:886402/1‑164 (MQ=255)                                                                                                                                                                                          tGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACat                                                 <  1:208846/251‑1 (MQ=255)                                                                                                                                                                                          tGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACat                                                 <  1:613969/251‑1 (MQ=255)                                                                                                                                                                                               cATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAAc                                            <  1:727154/251‑1 (MQ=255)                                                                                                                                                                                                aTGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGta                                                                                                                                                   <  1:641056/147‑1 (MQ=255)                                                                                                                                                                                                   gACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGAc                                                                >  1:381026/1‑227 (MQ=255)                                                                                                                                                                                                       ccATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAg                                                                                                                                               >  1:236418/1‑144 (MQ=255)                                                                                                                                                                                                       ccATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAAc                                            >  1:344350/1‑243 (MQ=255)                                                                                                                                                                                                       ccATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGgc                                       <  1:472252/248‑1 (MQ=255)                                                                                                                                                                                                            aTGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAacac                               <  1:286049/251‑1 (MQ=255)                                                                                                                                                                                                              gCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCt                                                                                          >  1:305454/1‑190 (MQ=255)                                                                                                                                                                                                              gCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAAc                                            <  1:840848/236‑1 (MQ=255)                                                                                                                                                                                                                     ccTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGt                                                                                                                                                    <  1:778944/125‑1 (MQ=255)                                                                                                                                                                                                                     ccTAAGCCTAAACGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCt                                                                                          <  1:270678/183‑1 (MQ=255)                                                                                                                                                                                                                              aaaCGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAAttc                                                                                                                      <  1:359436/146‑1 (MQ=255)                                                                                                                                                                                                                               aaCGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAatat            <  1:901906/251‑1 (MQ=255)                                                                                                                                                                                                                               aaCGGACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAatat            <  1:478201/251‑1 (MQ=255)                                                                                                                                                                                                                                   gACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTa                                                                                                                                             <  1:477152/118‑1 (MQ=255)                                                                                                                                                                                                                                   gACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAATATTGAg        >  1:629113/1‑251 (MQ=255)                                                                                                                                                                                                                                   gACATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAATATTGAg        >  1:634992/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCATAGGTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAATATTGAGc       <  1:197830/251‑1 (MQ=255)                                                                                                                                                                                                                                         ggTTTTGATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAATATTGAGCTTCCg  >  1:105494/1‑251 (MQ=255)                                                                                                                                                                                                                                               gATAACATTCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAAttc                                                                                                                      >  1:646052/1‑129 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             CATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGATAACATCCGCATCGGTAGCGGTAAATACAATATTCGGCAGGATACCGGCACCATTAAAGGCATAATCCAAATCGGAAACGCCGGTAAAGCCGAAAGTATAAGTAATTAAAGGATATTTGCCTAATTCTTCGATAGTCAGTAGCTCACATTTGGCTAACGGATGATCTGACTTCACAATGACCGAGCGATTCCACATATAACATGGCAGCAACACCACGCCATCAAATAAATATTGAGCTTCCG  >  1/1355834‑1356316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑