Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,355,833	A→G	intergenic (+196253/+65104)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,355,833	0	A	G	100.0%	140.4 / NA	50	intergenic (+196253/+65104)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/30);  total (20/30)

CCAAGCCTTCAATCACCTCTTCAACCGCCGCACGGGTTTGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑A‑‑GGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTACAATCTGACGTCATCAAATAATCTTTTCACCACATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGA  >  1/1355591‑1356073                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 ccAAGCCTTCAATCCCTTCTTCACCCGCCGCATGTGTTTGTACGTTAATTGTTTTTGTTGTAATTTCAGTGCTTTTTTCAAAAGCGTTTTAAAT‑AAGTTTTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCattat                                                                                                                                                                                                                                            <  1:373522/251‑1 (MQ=255) aacaGCCTTCTATCCCCCCTCCAACCGCGGCTTGGGTTTGTACGTCAATGTTTTTTGGTGTATTTTCAGTGCGTTTTAAAAAAGCTTTTTAAAT‑AAGTTGTTGTAAATTTAACATTTTT‑CTTCCTATAGAAATGTAAGAAAAAGGG‑‑GGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCattat                                                                                                                                                                                                                                            <  1:448571/248‑1 (MQ=255)      ctttCAATCCCCTCTTCAACCGCCGCACGGGTTTGTACGTAATTGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCg                                                                                                                                                                                                                                       <  1:622811/249‑1 (MQ=255)      ccTTCAATCACCTCTTCAACCGCCGCACGGGTTTGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCg                                                                                                                                                                                                                                       >  1:806928/1‑251 (MQ=255)        ttcaacccccctttCAACCGCCGCACGATTTTGTTCGTAAATTTTTTTTGGTTTAATTTCAGTGCTTTTTAAAAAATCTTTTTAAAT‑AAGTTTTTGTAAATTTGACATTTTG‑CTTCCTATATCAATTTTAGAAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTg                                                                                                                                                                                                                                     <  1:221628/239‑1 (MQ=255)        ttaattctccTCTACAACCGCCGCAGTGTTTTGTACGTAAATGTTTTTTGGTGTAATTTCAGTGTTTTTTACAAAAGCTTTTTAAAT‑AAGTTGTTGTAAATTGGGCTTTTTG‑CTTCCTATATAAATGTGAGAAAAAGGG‑‑GGGGGGGGGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTg                                                                                                                                                                                                                                     <  1:244908/243‑1 (MQ=255)        ttCAATCACCTCTTCAACCGCCGCACGGGTTTGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTg                                                                                                                                                                                                                                     >  1:536102/1‑251 (MQ=255)             cccccTCTTCAACCGCCGCACGGGTTTGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATAc                                                                                                                                                                                                                                <  1:29933/248‑1 (MQ=255)                 atgtgtttaaaagaggggggTTTGTACGTGAGTGGTTTTTGGTGTATTTTAATTGCTTTTTTAAAAGGCGTTTGAAAA‑ATGGTGTTGTAAATTTGACTTTTTG‑CATACTATATAAGTAGGAGAAAAAGGG‑‑GGGGGGGGGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGc                                                                                                                                                                                                                              <  1:133774/232‑1 (MQ=255)                     ccacccgccgCACGGGTTTGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTGGTTGTAAATTTGTCATTTTG‑CTTCCTATATAAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGcc                                                                                                                                                                                                                        <  1:140676/247‑1 (MQ=255)                                   ggTTTGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGtttt                                                                                                                                                                                                           >  1:770802/1‑250 (MQ=255)                                      ttGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACg                                                                                                                                                                                                       >  1:253814/1‑251 (MQ=255)                                             tCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAg                                                                                                                                                                                                >  1:487425/1‑251 (MQ=255)                                               atgggTTTTTGGTGTAATTGTAGTGAGTTTTTCAATAGAGTTTT‑AATAAAGTTGTTGTAATTTTGA‑TTTTTGTTTTCCTATATTAAGGTAAGTAAAAGGG‑‑GGGGGGGTGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTg                                                                                                                                                                                              <  1:328806/248‑1 (MQ=255)                                                             tAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTg                                                                                                                                                                                              >  1:398688/1‑237 (MQ=255)                                                                            tttCAAAATCTTTTTAAAT‑AAGTTGTTTTAAATTTGTTGTTTTT‑CTTCCTATATCAATTTTAGTAAAAGGG‑‑GGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTg                                                                                                                                                                                              <  1:95225/222‑1 (MQ=255)                                                                               aaaaaGCGTTTTCAAT‑AAGTTTTTGTAAATTTGACATTTTG‑CTTCCTATATAAATGTTAGAAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACatat                                                                                                                                                              <  1:777274/250‑1 (MQ=255)                                                                                  aagaaacaacAAT‑AAGTTTTTTTAAATTTTACATTTTG‑CTTCATATAGAAATGTTAGAAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAAt                                                                                                                                                           <  1:351842/242‑1 (MQ=255)                                                                                   aGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAAtt                                                                                                                                                          >  1:533749/1‑251 (MQ=255)                                                                                       ttttAAAT‑ATGTTGTTATAAAGTTGTCTTTTTG‑CTTCCAAAATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGc                                                                                                                                                      <  1:292920/251‑1 (MQ=255)                                                                                           cAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATcataca                                                                                                                                                                 >  1:688084/1‑236 (MQ=255)                                                                                              tcaggttgttGTAATTTTGACATTTTG‑CTTCCTATATAAATGTAAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGaa                                                                                                                                                 <  1:69374/246‑1 (MQ=255)                                                                                                                            tccaatatAAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAAt                                                                                                                                                           <  1:421314/207‑1 (MQ=255)                                                                                                                            tCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTa                                                                                                                                                         >  1:683959/1‑213 (MQ=255)                                                                                                                            tCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCAt                                                                                                                   >  1:672866/1‑251 (MQ=255)                                                                                                                            tCCTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCAt                                                                                                                   >  1:187663/1‑251 (MQ=255)                                                                                                                             ccTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATg                                                                                                                  >  1:556314/1‑251 (MQ=255)                                                                                                                              cTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATg                                                                                                                  >  1:383449/1‑250 (MQ=255)                                                                                                                              cTATATCAATGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGc                                                                                                                 <  1:613909/251‑1 (MQ=255)                                                                                                                              cTATATAAATGTAAGAAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTcc                                                                                                                                                                                                                  <  1:290236/154‑1 (MQ=255)                                                                                                                                       tGTTAGTAAAA‑‑AGGGGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGa                                                                                                        >  1:642887/1‑251 (MQ=255)                                                                                                                                                aaaaagggggggggCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATg                                                                                                 <  1:867162/244‑1 (MQ=255)                                                                                                                                                   aagggggggggCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGAt                                                                                               >  1:365144/5‑250 (MQ=255)                                                                                                                                                         gggggCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGt                                                                                                                                                                                                                                         >  1:37799/1‑106 (MQ=255)                                                                                                                                                           gggCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATg                                                                                                 >  1:522670/1‑240 (MQ=255)                                                                                                                                                              cGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGc                                                                                           >  1:577010/1‑243 (MQ=255)                                                                                                                                                               gggTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTg                                                                                   <  1:523159/250‑1 (MQ=255)                                                                                                                                                               gggTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTg                                                                                   <  1:111759/250‑1 (MQ=255)                                                                                                                                                                          gtTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGc                                                                                                                                                                                                                              <  1:95756/100‑1 (MQ=255)                                                                                                                                                                              cAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGTAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATc                                                                   <  1:409637/251‑1 (MQ=255)                                                                                                                                                                                                         ttCAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCAt                                        >  1:198974/1‑251 (MQ=255)                                                                                                                                                                                                           cAATTACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATaa                                      <  1:741424/251‑1 (MQ=255)                                                                                                                                                                                                              ttACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGc                                   <  1:464236/251‑1 (MQ=255)                                                                                                                                                                                                               tACAAAGTTTGCAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGcc                                  <  1:117563/251‑1 (MQ=255)                                                                                                                                                                                                                      tttgcAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACAcc                            <  1:184710/246‑1 (MQ=255)                                                                                                                                                                                                                          cAATCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGc                       <  1:271082/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCt                     <  1:85310/251‑1 (MQ=255)                                                                                                                                                                                                                            aTCTGACGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGAGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCt                     <  1:791035/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGTCATCAAATAATCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACg                <  1:762555/251‑1 (MQ=255)                                                                                                                                                                                                                                               tCTTTTCACCGCATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGa  >  1:191313/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 CCAAGCCTTCAATCACCTCTTCAACCGCCGCACGGGTTTGTACGTCAATGGTTTTTGGTGTAATTTCAGTGCGTTTTTCAAAAGCGTTTTCAAT‑AAGTTGTTGTAAATTTGACATTTTG‑CTTCCTATATCAATGTTAGTAAAA‑‑A‑‑GGGGGGGCGGGTGTTCGTCGTTGCAAATTCAATGTGCGAAAAGCCAACTTTTCAATTACAAAGTTTACAATCTGACGTCATCAAATAATCTTTTCACCACATTATTGTCGTGCATACGCTCTGCCCGTTCCACGTTTTCACGGGTTAAGTGCGGTGAAAAATAGTTGATAAAATCATACATATAATTACGCAGGAAAGCGCCGTGTTTAAAGGCGATTTGCGTCATGCTGGCGCGGAATAAATGATCCGCTTTAAGTGCCACCAAATCAACATCCGCCGGCGTATGCGCCATGGACGCCATAATGCCCACACCTAAGCCTAAACGGACATAGGTTTTGA  >  1/1355591‑1356073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑