Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,348,286	A→G	intergenic (+188706/+72651)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,348,286	0	A	G	100.0%	23.4 / NA	14	intergenic (+188706/+72651)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/0);  total (14/0)

ATTTGATAGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTATGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATAACTGGCAGGCATTTGATGGCGCCCAAAGAATTACGATTAAGACAAACCTCAACTATCAAGGGCAATA  >  1/1348066‑1348530                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     aTTTGATAGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCAGTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTgtgt                                                                                                                                                                                                                                                   <  1:684822/224‑1 (MQ=18)                                                                                                                                        tGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAg                                                                                 >  1:341697/1‑251 (MQ=11)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:416003/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:46835/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:528193/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:547462/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:739613/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:788928/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:810133/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:894489/1‑250 (MQ=14)                                                                                                                                                      gTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGCGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATaa                                                                    >  1:496910/1‑250 (MQ=14)                                                                                                                                                                       gAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATAAc                                                                   >  1:383881/1‑234 (MQ=17)                                                                                                                                                                       gAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATAAc                                                                   >  1:786596/1‑234 (MQ=17)                                                                                                                                                                       gAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATAACTGGCAGGCATTTGATgg                                                  >  1:578119/1‑251 (MQ=18)                                                                                                                                                                                                                       gTATTTGTGTTAGACCCGCCATATTTGTGTACGAAACAAGCGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATAACTGGCAGGCATTTGATGGCGCCCAAAGAATTACGATTAAGACAAACCTCAACTATCAAGGGCAata  >  1:822005/1‑251 (MQ=37)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     ATTTGATAGTTATAAGGATTTAAACTGCTTAGCCAGTTGGCTATTATTTAGCGGTCAGCAAGTCGCAACACTTAGTGATCTTTATCATAAAGATTTTTGGAATTGTATTAGAAAATCAGATTATCCAAATGCCCATGGTTATTTGGATGGTATAGAGATTACCAATGAATCATTCCACACGTTACTTCCGCGTTTTATCAATGACGAAAGGGCGGTATTTATGTTAGACCCGCCATATTTGTGTACGAAACAAGAGAGCTATAAGCAAGCGCATTATTTCGATCTTATCGACTTCCTACGCTTAATTAACATTACACGACCACCGTATATCTTCTTCTCATCGACAAAAAGCGAGTTCGTGCGGTTTATTGAGTATATGTGTGAAGATAAAGTCAATAACTGGCAGGCATTTGATGGCGCCCAAAGAATTACGATTAAGACAAACCTCAACTATCAAGGGCAATA  >  1/1348066‑1348530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑