Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,347,839	G→A	intergenic (+188259/+73098)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,347,839	0	G	A	100.0%	88.3 / NA	34	intergenic (+188259/+73098)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/18);  total (16/18)

CTTCATTTTGAAATAAGGTATAGTTATACTAATTTTTTATTTGATAAAGAACTTTTATTTGTTATCTACGCACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTGCGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATGCTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATGGTTATAGTGCCTTGAAAACCATCCAAAGAAATCGAACTATTTGATAGTTATAAGGAT  >  1/1347630‑1348084                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ctTCATTTTGAAATAAGGTATAGTTATACTAATTTTTTATTTGATAAAGAACTTTTATTTGTTATCTACGCACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTg                                                                                                                                                                                                              <  1:266754/251‑1 (MQ=255)                     taGTTATACTAATTTTTTATTTGATAAAGAACTTTTATTTGTTATCTACGCACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGt                                                                                                                                                                                                                                                    >  1:436592/1‑193 (MQ=255)                                                                 tCTACGCACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGa                                                                                                                                              <  1:595933/251‑1 (MQ=255)                                                                    aCGCACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGAt                                                                                                                                             >  1:873730/1‑249 (MQ=255)                                                                      gCACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGAtct                                                                                                                                           >  1:808041/1‑249 (MQ=255)                                                                       cACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAg                                                                                                                                                                                                                                     <  1:431436/158‑1 (MQ=255)                                                                          tataTCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTAGCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTAt                                                                                                                                     <  1:758282/251‑1 (MQ=255)                                                                             atCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGa                                                                                                                                              >  1:538900/1‑239 (MQ=255)                                                                                                       aaaataaaCCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACtt                                                                                                                                                                                                                                          <  1:391071/121‑1 (MQ=255)                                                                                                                         atatCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCt                                                                                                                                                                                       <  1:331543/154‑1 (MQ=255)                                                                                                                              atcatATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAg                                                                                                                                                                                                                                     >  1:232203/1‑103 (MQ=255)                                                                                                                              atcatATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGa                                                                                                                                              <  1:741156/190‑1 (MQ=255)                                                                                                                                catATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATaaa                                                                                                                                                                                                                     <  1:143898/117‑1 (MQ=255)                                                                                                                                      gTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATGCTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGa                                                                                                                                              <  1:870771/182‑1 (MQ=255)                                                                                                                                      gTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCt                                                                                                                                                                                       >  1:317342/1‑141 (MQ=255)                                                                                                                                              aCTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAAtt                                                                                                                                                                              <  1:656502/142‑1 (MQ=255)                                                                                                                                              aCTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTAt                                                                                                                                     <  1:618154/183‑1 (MQ=255)                                                                                                                                                   ttATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGa                                                                                                                                              <  1:287519/169‑1 (MQ=255)                                                                                                                                                     aTCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGt                                                                                                                                                                                                                                                    <  1:721417/65‑1 (MQ=255)                                                                                                                                                     aTCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTttac                                                                                                                          <  1:389162/187‑1 (MQ=255)                                                                                                                                                             atCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCAc                                                                                                          >  1:703871/1‑195 (MQ=255)                                                                                                                                                                    aCAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATAGTta                                                       >  1:834970/1‑239 (MQ=255)                                                                                                                                                                     cAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGa                                                                                                                                              >  1:607989/1‑151 (MQ=255)                                                                                                                                                                      aGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAAc                                                                                             >  1:808402/1‑199 (MQ=255)                                                                                                                                                                      aGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGt                                                                        <  1:91544/220‑1 (MQ=255)                                                                                                                                                                      aGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATAGTTATAGTGCCTTGaaaa                                         >  1:727808/1‑251 (MQ=255)                                                                                                                                                                       gTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATaaa                                                                                                                                                                                                                     >  1:179965/1‑78 (MQ=255)                                                                                                                                                                       gTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATAGTTATAGTGCCTTGaaaa                                         >  1:462281/1‑250 (MQ=255)                                                                                                                                                                                                   aaataaaCCAACCTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATAGTTATAGTGCCTTGAAAACCATCCAAAGAAATCGAACTATTTGATAg            >  1:202339/1‑251 (MQ=255)                                                                                                                                                                                                             accTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGa                                                                                                                                              <  1:583202/111‑1 (MQ=255)                                                                                                                                                                                                             accTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATAGTTATAGTGCCTTGAAAAc                                        <  1:670643/213‑1 (MQ=255)                                                                                                                                                                                                             accTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATAGTTATAGTGCCTTGAAAACCATCCAAAGAAATCGAACTATTTGATAGTTATAAGGAt  >  1:644796/1‑251 (MQ=255)                                                                                                                                                                                                             accTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATAGTTATAGTGCCTTGAAAACCATCCAAAGAAATCGAACTATTTGATAGTTATAAGGAt  >  1:493623/1‑251 (MQ=255)                                                                                                                                                                                                             accTTACGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATACTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATACAGTTATAGTGCCTTGAAAACCATCCAAAGAAATCGAACTATTTGATAGTTATAAgg    <  1:207292/249‑1 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CTTCATTTTGAAATAAGGTATAGTTATACTAATTTTTTATTTGATAAAGAACTTTTATTTGTTATCTACGCACTATATCAAATTCAATTTCTACATCAAAATAAAATAAACCATTTTGGTATATCATCATATAGTTAGATAACTCATTATCATATTATCTTCAACAGTTAATATTTTGCAAAATCGGCTTAAGTAAATAAACCAACCTTGCGTAGAATAACTTTTTAGTCACATTGTTCATAAATACGCTGTTTTAATAAATGCTCCTCTAGCTATCACAATTTATCAAATTTTGATAAGCGATCTGTTTACAGATCTCTGTATTGTAGTTTTACTTACTTACCAGTGCACTTGTGCCGATAACCTTAAAATCATTTTTGACAGTAATTGTACATATGGTTATAGTGCCTTGAAAACCATCCAAAGAAATCGAACTATTTGATAGTTATAAGGAT  >  1/1347630‑1348084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑