Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,346,583	A→G	intergenic (+187003/+74354)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,346,583	0	A	G	100.0%	185.8 / NA	64	intergenic (+187003/+74354)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/35);  total (29/35)

CCTTTTTGTCGCCTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTAGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGTATTAGGTTTGGCAACGCCGTTTGTACTCTTCTCTTTATTCTCACAAGAGTTA  >  1/1346339‑1346820                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              cctTTTTGTCGCCTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTggaaaga                                                                                                                                                                                                                                         >  1:358046/1‑251 (MQ=255) cctTTTTGTCGCCTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTggaaaga                                                                                                                                                                                                                                         >  1:778791/1‑251 (MQ=255) cctTTTTGTCGCCTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTCTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTggaaaga                                                                                                                                                                                                                                         >  1:422890/1‑251 (MQ=255)         tCGCCTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAagt                                                                                                                                                                                                                                 <  1:194538/251‑1 (MQ=255)         tCGCCTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAagt                                                                                                                                                                                                                                 <  1:18226/251‑1 (MQ=255)            ccTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTggaaag                                                                                                                                                                                                                                          >  1:673080/1‑239 (MQ=255)                        cccATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAAc                                                                                                                                                                                                                  <  1:128471/251‑1 (MQ=255)                                  cGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCaaa                                                                                                                                                                                                        <  1:417415/251‑1 (MQ=255)                                    atttatttCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAg                                                                                                                                                                                                      <  1:877462/251‑1 (MQ=255)                                           tCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTggaaag                                                                                                                                                                                                                                          >  1:848664/1‑208 (MQ=255)                                             ggCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCttt                                                                                                                                                                                             >  1:837812/1‑251 (MQ=255)                                                          aaGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTc                                                                                                                                                                                <  1:638989/251‑1 (MQ=255)                                                                 tAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGc                                                                                                                                                                         <  1:235894/251‑1 (MQ=255)                                                                 tAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGc                                                                                                                                                                         <  1:873809/251‑1 (MQ=255)                                                                      aaaTTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTTAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTAcc                                                                                                                                                                    <  1:215570/251‑1 (MQ=255)                                                                         ttATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTc                                                                                                                                                                 <  1:201167/251‑1 (MQ=255)                                                                           aTGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAAc                                                                                                                                                                                                                                       <  1:404301/179‑1 (MQ=255)                                                                                 gccTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTgg                                                                                                                                                         >  1:308279/2‑251 (MQ=255)                                                                                  ccTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAAc                                                                                                                                                                                                                                       <  1:70567/172‑1 (MQ=255)                                                                                  ccTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGcc                                                                                                                                                             >  1:812558/1‑246 (MQ=255)                                                                                    tttttGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGcc                                                                                                                                                             >  1:117058/1‑244 (MQ=255)                                                                                       ttGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGac                                                                                                                                                   <  1:666404/251‑1 (MQ=255)                                                                                         gTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAAc                                                                                                                                                                                                                  <  1:851346/186‑1 (MQ=255)                                                                                              ttGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGt                                                                                                                                            >  1:358026/1‑251 (MQ=255)                                                                                                   ggTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAg                                                                                                                                                                                                      <  1:461713/188‑1 (MQ=255)                                                                                                    gTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTc                                                                                                                                                                                >  1:866549/1‑209 (MQ=255)                                                                                                     tttATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGAc                                                                                                                                     <  1:698078/251‑1 (MQ=255)                                                                                                        aTCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTAtt                                                                                                                                         <  1:78439/244‑1 (MQ=255)                                                                                                         tCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTc                                                                                                                                                                 >  1:706982/1‑219 (MQ=255)                                                                                                               aCCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTAt                                                                                                                                          >  1:463287/1‑236 (MQ=255)                                                                                                               aCCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGc                                                                                                                           <  1:3510/251‑1 (MQ=255)                                                                                                                 cTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCAt                                                                                                                         >  1:804925/1‑251 (MQ=255)                                                                                                                   tCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGcc                                                                                                                                                             <  1:154482/213‑1 (MQ=255)                                                                                                                   tCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGAc                                                                                                                                     <  1:108122/237‑1 (MQ=255)                                                                                                                   tCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGAATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCAt                                                                                                                         >  1:208938/1‑249 (MQ=255)                                                                                                                    cGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCttt                                                                                                                                                                                             <  1:170449/180‑1 (MQ=255)                                                                                                                          ccTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTgg                                                                                                                >  1:515793/1‑251 (MQ=255)                                                                                                                            tAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCGGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCg                                                                                                              <  1:442052/251‑1 (MQ=255)                                                                                                                              ggCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCt                                                                                                                                                                   <  1:472216/196‑1 (MQ=255)                                                                                                                                           gATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCg                                                                                                              >  1:517070/1‑236 (MQ=255)                                                                                                                                                    gCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGc                                                                                       <  1:385909/250‑1 (MQ=255)                                                                                                                                                      aaTTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCt                                                                                                                                                            <  1:719636/179‑1 (MQ=255)                                                                                                                                                       aTTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGcc                                                                                                                                                             >  1:646822/1‑177 (MQ=255)                                                                                                                                                          tATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATAc                                                                                <  1:131658/251‑1 (MQ=255)                                                                                                                                                          tATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATAc                                                                                <  1:805417/251‑1 (MQ=255)                                                                                                                                                          tATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATAc                                                                                <  1:595463/251‑1 (MQ=255)                                                                                                                                                          tATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATAc                                                                                <  1:755430/251‑1 (MQ=255)                                                                                                                                                             tCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGt                                                                             >  1:674730/1‑251 (MQ=255)                                                                                                                                                               tttAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGc                                                                           <  1:462664/251‑1 (MQ=255)                                                                                                                                                                   aCGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAAc                                                                                                                                                                                                                                       <  1:600725/91‑1 (MQ=255)                                                                                                                                                                    cGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGc                                                                                                                                                                         >  1:162642/1‑152 (MQ=255)                                                                                                                                                                      aTAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTat                                                                     <  1:626162/250‑1 (MQ=255)                                                                                                                                                                                cATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCt                                                                                                                                                                   <  1:138680/146‑1 (MQ=255)                                                                                                                                                                                cATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTc                                                                                                                   >  1:505848/1‑194 (MQ=255)                                                                                                                                                                                  ttattGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAATGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGCATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGctc                                                                                     >  1:248999/1‑222 (MQ=255)                                                                                                                                                                                    attGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGAACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGt                                                      >  1:430496/1‑251 (MQ=255)                                                                                                                                                                                           gCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGacac                                                                                                                                                 >  1:406188/1‑153 (MQ=255)                                                                                                                                                                                               tATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCg                                                                                                              >  1:228972/1‑184 (MQ=255)                                                                                                                                                                                                aTCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATAc                                                                                <  1:479943/213‑1 (MQ=255)                                                                                                                                                                                                    tGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGTATTAGGTTTGGCAACg                                      >  1:365039/1‑251 (MQ=255)                                                                                                                                                                                                          ttCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGc                                                                                                                                                              >  1:212730/1‑125 (MQ=255)                                                                                                                                                                                                                gAATCCATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTAtt                                                                                                                                         >  1:867622/1‑140 (MQ=255)                                                                                                                                                                                                                    ccATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGTATTAGGTTTGGCAACGCCGTTTGTActctt                        >  1:7232/1‑249 (MQ=255)                                                                                                                                                                                                                    ccATCAATTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGTATTAGGTTTGGCAACGCCGTTTGTAc                            >  1:678164/1‑245 (MQ=255)                                                                                                                                                                                                                         aaTTAGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTa                                                                      >  1:249889/1‑198 (MQ=255)                                                                                                                                                                                                                             aGGTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGTATTAGGTTTGGCAACGCCGTTTGTAc                            >  1:465854/1‑236 (MQ=255)                                                                                                                                                                                                                               gTATTATCAAATTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCg                                                                                                              <  1:639583/152‑1 (MQ=255)                                                                                                                                                                                                                                         aTTGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGTATTAGGTTTGGCAACGCCGTTTGTACTCTTCTCTTTATTCTCACAAGAGTTa  >  1:763627/1‑250 (MQ=255)                                                                                                                                                                                                                                          ttGAATTTCCTGGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCtt                                                                                                                                                                                              >  1:852334/1‑61 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              CCTTTTTGTCGCCTTGCATTTTCCCCATTATCCCGATTTATTTCGGCATCTTGGCGAAAGGCGGTAGAAAAATTATGAACACCTTTTTGTTCATTGCAGGTTTATCTTTAACCTTCGTCAGCCTAGGCTTCAGCTTCGGATTTTTAGGCAATTTATTCTTTAACGATAACGTGCGCATTATTGCCGGCATTATCGTGATTATTCTGGGAATCCATCAATTAGGTATTATCAAATTGAATTTCCTAGAAAGAACCAAAGTAGTTGAAGTAAAAACTGAAGGCAAAAGTGCCTCTTTTGAAGCCTTTTTCCTAGGGCTTACCTTCAGCCTTGGCTGGACACCTTGTATTGGACCGATTTTAGCATCTGTTCTGGCGTTGTCCGGCGATGAAGGTTCCGCTCTATACGGTGCTTCTATGATGTTGGTTTATGTATTAGGTTTGGCAACGCCGTTTGTACTCTTCTCTTTATTCTCACAAGAGTTA  >  1/1346339‑1346820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑