Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,342,849	T→C	intergenic (+183269/+78088)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,342,849	0	T	C	100.0%	113.9 / NA	43	intergenic (+183269/+78088)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/31);  total (12/31)

GATTTGAACAAAAAGCTCGCCTCTTTTTAGAATCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCATAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACAAATCGTCGAAGCGACGCAACACCTGATTGCCTGCCTGTTGCGTGGTAATAAAGT  >  1/1342622‑1343093                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gaTTTGAACAAAAAGCTCGCCTCTTTTTAGAATCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTagaaga                                                                                                                                                                                                                                >  1:665067/1‑250 (MQ=255)  aTTTGAACAAAAAGCTCGCCTCTTTTTAGAATCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGac                                                                                                                                                                                                                               <  1:97851/250‑1 (MQ=255)  aTTTGAACAAAAAGCTCGCCTCTTTTTAGAATCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGac                                                                                                                                                                                                                               <  1:149436/250‑1 (MQ=255)          aaaaaGCTCGCCTCTTTTTAGAATCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGc                                                                                                                                                                                                                      <  1:188626/251‑1 (MQ=255)                  cGCCTCTTTTTAGAATCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAAc                                                                                                                                                                                                                                                   <  1:509801/214‑1 (MQ=255)                                aTCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTg                                                                                                                                                                                                >  1:656361/1‑251 (MQ=255)                                        gCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAAc                                                                                                                                                                                         <  1:889754/250‑1 (MQ=255)                                        gCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAAc                                                                                                                                                                                         <  1:608480/250‑1 (MQ=255)                                         cATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAAcc                                                                                                                                                                                        <  1:874181/250‑1 (MQ=255)                                                tttCTGGCCGCCAACAAAAATTTCAAGGGGGCCGAATTGGATTTAATTATTTTGGACCAAAATCCAATGGTATTGGTGGAAGTGAGCC‑ACGGAAAAATTGATCATTTTGGACCTGCCGTGGAAAGCGAAGATTGCCAAAACCATCAAAGATGCCTGAATCCGGCAG‑ACCTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGAc                                                                                                                                                                               <  1:276079/251‑1 (MQ=255)                                                           caaccaaAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATgg                                                                                                                                                                      <  1:717170/250‑1 (MQ=255)                                                                    tttCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCaa                                                                                                                                                              >  1:421000/1‑249 (MQ=255)                                                                                       gATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCttt                                                                                                                                         >  1:416427/1‑251 (MQ=255)                                                                                        aTTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCttt                                                                                                                                         >  1:120046/1‑250 (MQ=255)                                                                                          ttAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCAc                                                                                                                                      <  1:834545/251‑1 (MQ=255)                                                                                                aTGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCa                                                                                                                                >  1:419692/1‑251 (MQ=255)                                                                                                 tGTTGGAACAAAATCCCATCGTATTTGTGGAAGTGCGCCACCGT‑AAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAAc                                                                                                                                                                                         <  1:157688/192‑1 (MQ=255)                                                                                                           aaaTACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACtt                                                                                                                                                           <  1:278949/213‑1 (MQ=255)                                                                                                                ccATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCAc                                                                                                                                      <  1:430980/229‑1 (MQ=255)                                                                                                                ccATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGAt                                                                                                                <  1:739303/251‑1 (MQ=255)                                                                                                                   tCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTc                                                                                                                      <  1:865731/242‑1 (MQ=255)                                                                                                                      tATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTatat                                                                                                          <  1:360129/251‑1 (MQ=255)                                                                                                                      tATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTatat                                                                                                          <  1:444911/251‑1 (MQ=255)                                                                                                                       aTTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATg                                                                                                         <  1:372055/251‑1 (MQ=255)                                                                                                                         ttGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAAcc                                                                                                                                                                                        <  1:750009/170‑1 (MQ=255)                                                                                                                         ttGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATgg                                                                                                                                                                      <  1:389175/188‑1 (MQ=255)                                                                                                                         ttGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAg                                                                                                       >  1:839190/1‑251 (MQ=255)                                                                                                                         ttGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAg                                                                                                       >  1:635223/1‑251 (MQ=255)                                                                                                                                       cAACGTAAAAA‑TGATCATTTTGGGTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACGACGCTGGCTGAATGCAGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCATCTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAAc                                                                                         <  1:161373/251‑1 (MQ=255)                                                                                                                                        aaCGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACt                                                                                        <  1:546860/251‑1 (MQ=255)                                                                                                                                              aaaa‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAAc                                                                                                                                                             >  1:596829/1‑176 (MQ=255)                                                                                                                                               aaa‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCAtattt                                                                                 <  1:144304/251‑1 (MQ=255)                                                                                                                                                                  cgCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTc                                                                <  1:103637/249‑1 (MQ=255)                                                                                                                                                                   gCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAaaac                                                                                                                            <  1:35991/189‑1 (MQ=255)                                                                                                                                                                   gCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAg                                                              >  1:457997/1‑251 (MQ=255)                                                                                                                                                                           aaGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACaaa                                                      <  1:321704/251‑1 (MQ=255)                                                                                                                                                                                   aTTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACAAATCGTCGa                                               <  1:21470/250‑1 (MQ=255)                                                                                                                                                                                           aaGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGAc                                                                                                                                                                               <  1:896484/114‑1 (MQ=255)                                                                                                                                                                                                       cctccgatggCGGCAGCACTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACAAATCGTCGAAGCGACGc                                       >  1:239407/10‑238 (MQ=255)                                                                                                                                                                                                                        aCTTTGGCTGGCACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCAc                                                                                                                                      >  1:524985/1‑126 (MQ=255)                                                                                                                                                                                                                               ctggcACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACAAATCGTCGAAGCGACGCAACACCTGATTGCCTGCCTGTTGCGTGGTAATAAAg   <  1:825833/250‑1 (MQ=255)                                                                                                                                                                                                                               ctggcACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACAAATCGTCGAAGCGACGCAACACCTGATTGCCTGCCTGTTGCGTGGTAATAAAg   <  1:62589/250‑1 (MQ=255)                                                                                                                                                                                                                               ctggcACAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACAAATCGTCGAAGCGACGCAACACCTGATTGCCTGCCTGTTGCGTGGTAATAAAGt  <  1:213425/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GATTTGAACAAAAAGCTCGCCTCTTTTTAGAATCTAAAGGCATGAAATTTCTCGCCGCCAACCAAAATTTCAAGTGCGGCGAACTGGATTTAATTATGTTGGAACAAAATACCATCGTATTTGTGGAAGTGCGCCAACGTAAAAA‑TGATCATTTTGGCTCTGCCGTGGAAAGCGTAGATTGGCAAAAGCAACAACGCTGGCTGAATGCGGCAGCACTTTGGCTGGCATAACGGGAACAAAGTTTAGAAGACACCGATTGCCGTTTCGATTTGGTCGCGTTTGGTAAAACCGATCAAGACATTCAATGGATCTCCAACTTTCTTGATTAATATTCTTTCACTATGCAAAACAAACTCAATGATTTATATGAGGAAAATATTAAAACTCATATTTATTCAAAAAGCCTGCTCAGCGCACAAATCGTCGAAGCGACGCAACACCTGATTGCCTGCCTGTTGCGTGGTAATAAAGT  >  1/1342622‑1343093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑