Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,339,239	A→G	intergenic (+179659/+81698)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,339,239	0	A	G	100.0%	194.2 / NA	70	intergenic (+179659/+81698)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/37);  total (33/37)

CGGTGGCGTTAATCACTGCCGTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGATAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCACTAACGCCACATCGCCGGCCGCCAAATAACGCACGCCGCCGT  >  1/1338996‑1339484                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      cGGTGGCGTTAATCACTGCCGTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGc                                                                                                                                                                                                                                                <  1:830595/251‑1 (MQ=255)           aatgaCTGCCGTACCATGCACGCTATATTGTGCGCCGTTGCAGGCATCGGTAAATGTAACGCCGTTGATTTTATCTTGCCCAGCGGTGAACAACTAGGTCAATTTCGCATGAGTGGGCACGGTACCGCCTAGGGCGACCACGGTTTGAGAGATATTTATAGCAAGGCGGGAATCGGCAAACTGACCGTCTTGATATACCACGCGGTTTTTCAAGTGAGTTCCGTTTAAGGTGGGGAAACGCTGCAtggtgg                                                                                                                                                                                                                                      <  1:738537/247‑1 (MQ=255)           aaTCACTGCCGTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCgtgg                                                                                                                                                                                                                                      <  1:341884/251‑1 (MQ=255)           aaTCACTGCCGTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCgtgg                                                                                                                                                                                                                                      >  1:482972/1‑251 (MQ=255)                  gCCGTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCAt                                                                                                                                                                                                                                           <  1:603435/239‑1 (MQ=255)                  gCCGTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCttt                                                                                                                                                                                                                               >  1:281212/1‑251 (MQ=255)                     gTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTttatt                                                                                                                                                                                                                            >  1:817114/1‑251 (MQ=255)                      tACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTttatt                                                                                                                                                                                                                            <  1:570118/250‑1 (MQ=255)                       aCCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGc                                                                                                                                                                                                                                                >  1:835754/1‑229 (MQ=255)                        ccATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAAt                                                                                                                                                                                                                         <  1:527497/251‑1 (MQ=255)                               cGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATg                                                                                                                                                                                                                  >  1:59772/1‑251 (MQ=255)                                 cTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCAt                                                                                                                                                                                                                                           <  1:646939/224‑1 (MQ=255)                                 cTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGt                                                                                                                                                                                                                >  1:827893/1‑251 (MQ=255)                                 cTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGt                                                                                                                                                                                                                >  1:4336/1‑251 (MQ=255)                                   atatTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGttt                                                                                                                                                                                                              >  1:812012/1‑251 (MQ=255)                                   atatTGTTCGCCGTTTAATTCATCGGTAAATTTAACGACGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGttt                                                                                                                                                                                                              >  1:642247/1‑251 (MQ=255)                                         ttCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTGCCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTAccc                                                                                                                                                                                                         <  1:539293/250‑1 (MQ=255)                                                   aaTTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATc                                                                                                                                                                                              <  1:21013/251‑1 (MQ=255)                                                                          gTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAAc                                                                                                                                                                                                     >  1:831772/1‑221 (MQ=255)                                                                                 ttACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTTAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATAcaaccccaaccc                                                                                                                                                                <  1:875090/251‑1 (MQ=255)                                                                                 ttACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATAcaaccccaaccc                                                                                                                                                                <  1:51137/251‑1 (MQ=255)                                                                                 ttACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATAcaaccccaaccc                                                                                                                                                                <  1:432838/251‑1 (MQ=255)                                                                                   aCCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATAcaaccccaaccc                                                                                                                                                                <  1:544082/249‑1 (MQ=255)                                                                                      ttGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGacac                                                                                                                                                           <  1:143690/251‑1 (MQ=255)                                                                                             tCCTTTAACAATTCAGTCACTTTCGCATAATTAAGCGCGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTaa                                                                                                                                                                                                                          <  1:425044/181‑1 (MQ=255)                                                                                             tCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCAt                                                                                                                                                                                                                                           >  1:765941/1‑164 (MQ=255)                                                                                             tCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCAt                                                                                                                                                                             >  1:219847/1‑226 (MQ=255)                                                                                                tttAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCAc                                                                                                                                                 <  1:223197/251‑1 (MQ=255)                                                                                                tttAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCAc                                                                                                                                                 <  1:496980/251‑1 (MQ=255)                                                                                                tttAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCAc                                                                                                                                                 <  1:155759/251‑1 (MQ=255)                                                                                                 ttAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACg                                                                                                                                                <  1:511009/251‑1 (MQ=255)                                                                                                  tAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATGCCACGTCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGc                                                                                                                                               <  1:632277/251‑1 (MQ=255)                                                                                                  tAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGc                                                                                                                                               <  1:152232/251‑1 (MQ=255)                                                                                                                cTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGaca                                                                                                                                                            <  1:803621/224‑1 (MQ=255)                                                                                                                   tCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAg                                                                                                                              <  1:225260/251‑1 (MQ=255)                                                                                                                          aTTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATAcaaccccaaccc                                                                                                                                                                <  1:615655/210‑1 (MQ=255)                                                                                                                          aTTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGaa                                                                                                                       >  1:269413/1‑251 (MQ=255)                                                                                                                             aaGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTttat                                                                                                                                                                                                                             >  1:816007/1‑146 (MQ=255)                                                                                                                             aaGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGaataa                                                                                                                    >  1:42617/1‑251 (MQ=255)                                                                                                                                  cGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATAcaacccc                                                                                                                                                                     <  1:651477/197‑1 (MQ=255)                                                                                                                                   ggTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTCGGAATAATACAAt                                                                                                              >  1:284195/1‑251 (MQ=255)                                                                                                                                            ttGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGAtttt                                                                                                     >  1:560747/1‑251 (MQ=255)                                                                                                                                            ttGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGAtttt                                                                                                     >  1:277744/1‑251 (MQ=255)                                                                                                                                              gTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAg                                                                                                                              >  1:599315/1‑224 (MQ=255)                                                                                                                                              gTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTg                                                                                                   >  1:856791/1‑251 (MQ=255)                                                                                                                                                 cAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAAc                                                                                                <  1:657304/251‑1 (MQ=255)                                                                                                                                                       cGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTgg                                                                                          >  1:130326/1‑251 (MQ=255)                                                                                                                                                                  atatTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACg                                                                                                                                                <  1:686878/186‑1 (MQ=255)                                                                                                                                                                  atatTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAAt                                                                                                                                   >  1:279484/1‑199 (MQ=255)                                                                                                                                                                    atTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGGCACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTg                                                                             >  1:449667/1‑251 (MQ=255)                                                                                                                                                                    atTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAAtaataa                                                                                                                 <  1:228217/215‑1 (MQ=255)                                                                                                                                                                    atTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGAcg                                                                                      <  1:300434/242‑1 (MQ=255)                                                                                                                                                                               aGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACg                                                                                                                                                <  1:197454/173‑1 (MQ=255)                                                                                                                                                                                       tCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACg                                                          >  1:619974/1‑251 (MQ=255)                                                                                                                                                                                        cGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGc                                                         <  1:793386/251‑1 (MQ=255)                                                                                                                                                                                           cAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATAcaaccccaaccc                                                                                                                                                                <  1:253866/145‑1 (MQ=255)                                                                                                                                                                                                      tCTTTATATACCACGCCGTTTTTCAATTCCGTTCCGTTTAAGGTTGGTAAACTCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCa                                            >  1:613215/1‑250 (MQ=255)                                                                                                                                                                                                      tCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCtt                                               <  1:874567/247‑1 (MQ=255)                                                                                                                                                                                                      tCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCa                                            >  1:565381/1‑250 (MQ=255)                                                                                                                                                                                                      tCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCa                                            >  1:519763/1‑250 (MQ=255)                                                                                                                                                                                                       cTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAg                                                                                                                              >  1:24525/1‑167 (MQ=255)                                                                                                                                                                                                       cTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCa                                            >  1:273107/1‑249 (MQ=255)                                                                                                                                                                                                        tttATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCACTa                                         >  1:485063/1‑251 (MQ=255)                                                                                                                                                                                                         ttATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTAccc                                                                                                                                                                                                         <  1:745528/90‑1 (MQ=255)                                                                                                                                                                                                                       gTTTTTCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCaa                                                       >  1:206607/1‑222 (MQ=255)                                                                                                                                                                                                                        tttttCAAGTCCGTTCCGTTTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAAc                                                                                                <  1:123648/180‑1 (MQ=255)                                                                                                                                                                                                                                     ttccgttTTAAGTTGGTAAACCCTTCATCCTTGTTTCCTTATTAAACGCCATGGTTTTTCCCCAACCTAAATCCCCGGACAAATAAACAAACAACCCCAACCCCACCCGGTTTTTCACGCCGTCCAAAAAATTATAGTTAGGAATAATAAAATTCTTAATTTTGAACCGGTGGGACCCCTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCACTAACGCCACATCGCCGGCCGCCAAATAacgc            <  1:147203/251‑1 (MQ=255)                                                                                                                                                                                                                                         gttTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGaa                                                                                                                       <  1:26493/140‑1 (MQ=255)                                                                                                                                                                                                                                         gttTAAGGTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCACTAACGCCACATCGCCGGCCGCCAAATAacgcacg         >  1:251805/1‑250 (MQ=255)                                                                                                                                                                                                                                               ggTTGGTAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCACTAACGCCACATCGCCGGCCGCCAAATAACGCACGCCGCCGt  >  1:416089/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      CGGTGGCGTTAATCACTGCCGTACCATGCACGCTATATTGTTCGCCGTTTAATTCATCGGTAAATTTAACGCCGTTGATTTTACCTTGCCCGTCCTTTAACAACTCAGTCACTTTCGCATAATTAAGCACGGTACCGCCTTGTTCAACCACGGTTTGCGCGATATTTATCGCCAAGCGGGAATCGTCAAACTGACCGTCTTTATATACCACGCCGTTTTTCAAGTCCGTTCCGTTTAAGGTTGATAAACGCTGCATCGTGGTGTCTTTATTAATCGCGATGGTTTTACCCAAACTTAAATCGCCGGACAAATAATCATACAACCCCAACCCGACACGGTATTTCACGCTGTCGATAAAATTATAGTTAGGAATAATAAAATTCTGATTTTTGAACAGGTGGGACGCGTTTTTTGCTAAACGACCACGTTCACGCAACGCCTCTTTCACTAACGCCACATCGCCGGCCGCCAAATAACGCACGCCGCCGT  >  1/1338996‑1339484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑