Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,336,137	C→T	intergenic (+176557/+84800)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,336,137	0	C	T	100.0%	139.7 / NA	52	intergenic (+176557/+84800)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/22);  total (30/22)

AAAATCCAGTATTCTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AA‑CCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACCGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTAGGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCAT  >  1/1335892‑1336383                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aaaaTCCAGTATTCTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATg                                                                                                                                                                                                                                                    >  1:8760/1‑251 (MQ=255)      ccAGTATTCTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATaa                                                                                                                                                                                                                                               <  1:535115/251‑1 (MQ=255)      ccAGTATTCTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATaa                                                                                                                                                                                                                                               <  1:396432/251‑1 (MQ=255)         gTATTCTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATaa                                                                                                                                                                                                                                               <  1:456721/248‑1 (MQ=255)           aTTCTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTa                                                                                                                                                                                                                                          >  1:801858/1‑251 (MQ=255)              cTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCTGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGtat                                                                                                                                                                                                                                                 <  1:448730/241‑1 (MQ=255)              cTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAAt                                                                                                                                                                                                                                              >  1:727525/1‑244 (MQ=255)              cTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAata                                                                                                                                                                                                                                       <  1:96800/251‑1 (MQ=255)              cTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAata                                                                                                                                                                                                                                       >  1:533218/1‑251 (MQ=255)               tGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAatat                                                                                                                                                                                                                                      <  1:341127/251‑1 (MQ=255)                        gTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCa                                                                                                                                                                                                                             >  1:616616/1‑251 (MQ=255)                        gTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCATACCCa                                                                                                                                                                                                                             >  1:213260/1‑251 (MQ=255)                         ttCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTAccc                                                                                                                                                                                                                              <  1:51510/249‑1 (MQ=255)                         ttCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTAccc                                                                                                                                                                                                                              <  1:171802/249‑1 (MQ=255)                                 ccAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCACGAC‑CCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAg                                                                                                                                                                                                                    >  1:595743/1‑251 (MQ=255)                                 ccAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAAtt                                                                                                                                                                                                                                             >  1:246759/1‑226 (MQ=255)                                 ccAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAAt                                                                                                                                                                                                                                              <  1:821162/225‑1 (MQ=255)                                        atTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTAtttt                                                                                                                                                                                                             >  1:756999/1‑251 (MQ=255)                                                               agcTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGt                                                                                                                                                                                      >  1:63265/1‑251 (MQ=255)                                                                 cTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTaa                                                                                                                                                                                    >  1:366602/1‑251 (MQ=255)                                                                       ggCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACAttt                                                                                                                                                                               >  1:776473/1‑250 (MQ=255)                                                                            aaaTATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATaa                                                                                                                                                                                                                                               <  1:851609/181‑1 (MQ=255)                                                                                             aTCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATg                                                                                                                                                                                                                                                    >  1:270051/1‑159 (MQ=255)                                                                                             aTCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATg                                                                                                                                                                                                                                                    <  1:691875/159‑1 (MQ=255)                                                                                             aTCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAat                                                                                                                                                                           >  1:415112/1‑232 (MQ=255)                                                                                             aTCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGc                                                                                                                                                             >  1:788540/1‑246 (MQ=255)                                                                                             aTCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGa                                                                                                                                                        >  1:630541/1‑251 (MQ=255)                                                                                                                 ccccACTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCaaaa                                                                                                                                    >  1:569148/1‑251 (MQ=255)                                                                                                                     aCTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGc                                                                                                                                <  1:746668/251‑1 (MQ=255)                                                                                                                      cTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATaa                                                                                                                                                                                                                                               >  1:31583/1‑139 (MQ=255)                                                                                                                      cTTTGGTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGc                                                                                                                                >  1:85524/1‑250 (MQ=255)                                                                                                                           gTGCCACTGCCAC‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACa                                                                                                                                                                                  <  1:342941/195‑1 (MQ=255)                                                                                                                                 ctgccac‑AACCCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATAc                                                                                                                                                                       <  1:681095/200‑1 (MQ=255)                                                                                                                                        caacccGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACgg                                                                                                                                                                     <  1:809994/192‑1 (MQ=255)                                                                                                                                        caacccGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGc                                                                                                                    >  1:885242/5‑245 (MQ=255)                                                                                                                                                        gTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTc                                                                                                             >  1:175886/1‑236 (MQ=255)                                                                                                                                                            aCCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAAc                                                                                                                                            <  1:804799/201‑1 (MQ=255)                                                                                                                                                            aCCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAaat                                                                                           >  1:411984/1‑250 (MQ=255)                                                                                                                                                                         gaagaaAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGata                                                                              <  1:313920/250‑1 (MQ=255)                                                                                                                                                                                     ttattGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATa                                                                 >  1:42178/1‑251 (MQ=255)                                                                                                                                                                                          ggCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGAt                                                                                                                                                                                                          >  1:358908/1‑109 (MQ=255)                                                                                                                                                                                          ggCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTa                                                                                                                      >  1:96982/1‑193 (MQ=255)                                                                                                                                                                                              ttATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAAt                                                        <  1:428907/251‑1 (MQ=255)                                                                                                                                                                                                aTCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACAt                                                                                                                                                                                 >  1:112032/1‑128 (MQ=255)                                                                                                                                                                                                       gaATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAAc                                                                                                                                            <  1:506337/158‑1 (MQ=255)                                                                                                                                                                                                                              cGCAAACACCATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGAtgtg                         >  1:678912/1‑250 (MQ=255)                                                                                                                                                                                                                                      ccATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGa                             <  1:146708/238‑1 (MQ=255)                                                                                                                                                                                                                                      ccATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCgtg                >  1:337106/1‑251 (MQ=255)                                                                                                                                                                                                                                      ccATTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCgtg                >  1:18809/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTTATCATCAATGCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAata                                                                                                                                                                          <  1:320006/95‑1 (MQ=255)                                                                                                                                                                                                                                                    gCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATg                                                       <  1:663069/198‑1 (MQ=255)                                                                                                                                                                                                                                                    gCACTGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTGAGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCAt  >  1:819573/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AAAATCCAGTATTCTGGCAGACAGTTCACAAACCAATCAATTATCTGATTATTTGGAAAGCAAGCTTACAGGCAAAAATATCGCCGTGCGCGATCTTGCAGTAAATCAACTTCCCCACTTTGGTGCCACTGCCAC‑AA‑CCGCCCTACGCGGTCAACCGACGACAGCGGAAGAAAACGCATTATTGGCGTTATCTGATGAATTAGTCGCCGAAATTAAAAACGCAAACACCATTATCATCAATGCACCGATGTATAATTTTAATATCCCTACCCAATTAAAAAGCTATTTTGATTTCATTGCACGCCCTCGCGTAACATTTCAATATACGGAGGAAGGCCCTGAAGGATTATTAACAGGCAAAAAAGCCGTGGTATTAGCTGTATTCGGCGGGTTCCACCAAAATCAATCTTCCGATATCGTCACGGCATACTTAAAAATGATTTTAGGTTTCGTAGGTATTACCGATGTGCAATTCGTGTATGCAGAAGGCAT  >  1/1335892‑1336383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑