Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,335,674	C→A	intergenic (+176094/+85263)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,335,674	0	C	A	100.0%	107.1 / NA	39	intergenic (+176094/+85263)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/23);  total (16/23)

CTTTTTCCGCTTTTTTCTCGGCTTTTTCCGCCGCTTTTAATTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGACCACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTAGGAAGGAACAAAATGAACAACGTTCTTGTATTAAAATCCAGTATTCTGGCAGACAGTTCAC  >  1/1335433‑1335920                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       cTTTTTCCGCTTTTTTCTCGGCTTTTTCCGCCGCTTTTAATTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAAtt                                                                                                                                                                                                                                               <  1:146969/251‑1 (MQ=255)       ccGCTTTTTTCTCGGCTTTTTCCGCCGCTTTTAATTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCt                                                                                                                                                                                                                                         <  1:2124/251‑1 (MQ=255)                                 gcTTTTAATTCGTCTTCGGTGAGATGAAAATCGATTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGAtt                                                                                                                                                                                                               >  1:428263/1‑251 (MQ=255)                                      tAATTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAgg                                                                                                                                                                                                          <  1:585945/251‑1 (MQ=255)                                      tAATTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAgg                                                                                                                                                                                                          <  1:609137/251‑1 (MQ=255)                                        aTTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGgag                                                                                                                                                                                                        <  1:35171/251‑1 (MQ=255)                                        aTTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGgag                                                                                                                                                                                                        <  1:75669/251‑1 (MQ=255)                                             tCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGcc                                                                                                                                                                                                   <  1:459724/251‑1 (MQ=255)                                                     gagaTGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAag                                                                                                                                                                                                                         >  1:662098/1‑221 (MQ=255)                                                       gaTGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAAc                                                                                                                                                                                                                            >  1:70862/1‑216 (MQ=255)                                                       gaTGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAagag                                                                                                                                                                                                                       <  1:375772/221‑1 (MQ=255)                                                                 gCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAgg                                                                                                                                                                                                          >  1:462107/1‑224 (MQ=255)                                                                      aTTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGAtt                                                                                                                                                                                                               <  1:345300/214‑1 (MQ=255)                                                                              ccATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGc                                                                                                                                                                                       >  1:805478/1‑230 (MQ=255)                                                                                           aTTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAAcc                                                                                                                                                     <  1:704172/251‑1 (MQ=255)                                                                                                  ggATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTcc                                                                                                                                                                                                                                          >  1:302335/1‑159 (MQ=255)                                                                                                          cGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGc                                                                                                                                      <  1:59523/251‑1 (MQ=255)                                                                                                           ggTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGAt                                                                                                                                                                                                                <  1:348186/176‑1 (MQ=255)                                                                                                                 cACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTcc                                                                                                                                                                                              >  1:828248/1‑188 (MQ=255)                                                                                                                 cACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAAt                                                                                                                                           <  1:786195/239‑1 (MQ=255)                                                                                                                   ccAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAag                                                                                                                                                                                                                         >  1:287623/1‑159 (MQ=255)                                                                                                                   ccAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAgg                                                                                                                                                                                                          >  1:839679/1‑174 (MQ=255)                                                                                                                   ccAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAg                                                                                                                                         >  1:194651/1‑239 (MQ=255)                                                                                                                   ccAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCaaaa                                                                                                                             >  1:361033/1‑251 (MQ=255)                                                                                                                             gTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAg                                                                                                                                         <  1:124402/229‑1 (MQ=255)                                                                                                                                                        tGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATg                                                                                        <  1:512609/251‑1 (MQ=255)                                                                                                                                                         gCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGAtt                                                                                                                                                                                                               <  1:26442/131‑1 (MQ=255)                                                                                                                                                         gCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGAtt                                                                                                                                                                                                               >  1:124401/1‑131 (MQ=255)                                                                                                                                                         gCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAGATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGa                                                                                       >  1:442166/1‑251 (MQ=255)                                                                                                                                                          cAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGAcc                                                                                                                                                                                                                    <  1:9552/125‑1 (MQ=255)                                                                                                                                                                         ccACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCGCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCGTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACtca                                                                       <  1:79627/251‑1 (MQ=255)                                                                                                                                                                                    aTCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTagga                                                            <  1:38117/251‑1 (MQ=255)                                                                                                                                                                                        caAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAAtttt                                                                                                                                                                                                                                             >  1:844103/1‑70 (MQ=255)                                                                                                                                                                                        caAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTaggaagga                                                        >  1:774157/1‑251 (MQ=255)                                                                                                                                                                                         aaGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAg                                                                                                                                         >  1:826543/1‑169 (MQ=255)                                                                                                                                                                                                    tGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTAGGAAGGAACAAAATGaac                                             <  1:170477/250‑1 (MQ=255)                                                                                                                                                                                                                           ccATAACCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCTTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTAGGAAGGAACAAAATGAACAACGTTCTTGTATTAAAATCCAGt                     <  1:430975/251‑1 (MQ=255)                                                                                                                                                                                                                               aaCCAATAAAAATTTCCGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTAGGAAGGAACAAAATGAACAACGTTCTTGTATTAAAATCCAGTATTc                 <  1:245892/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccGAACACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTAGGAAGGAACAAAATGAACAACGTTCTTGTATTAAAATCCAGTATTCTGGCAGACAGTTcac  <  1:51541/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       CTTTTTCCGCTTTTTTCTCGGCTTTTTCCGCCGCTTTTAATTCGTCTTCGGTGAGATGAAAATCGCTTAATTTTTTACCATTTTCCTCAAATTCCTTGGATAAATCGGTAATCACCAATTCACCGTGCAAATGCGCTTTTTTCTGTTTAATTGCAATAATCATCGCGACCACGGCGGCGATCACAAGTAATAGCGTGATAACTTCCAAAAGAAATACACCATAACCAATAAAAATTTCCGACCACATAATTTTTCCTTCATATTATTAACAAGAGACCAGATTATAGGAGAAGCCTTTCCTTAAAGCTACTTTATCTCTCAAATAAATCGGATTATTAACCCATCGTTAATAGTGCTTTTCAAAAGAAAAGCCTTTATTTAAAAAAAACATTCGTTAAAATGACCGCACTTTTCACTCATTCATTTAGGAAGGAACAAAATGAACAACGTTCTTGTATTAAAATCCAGTATTCTGGCAGACAGTTCAC  >  1/1335433‑1335920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑