Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,334,570 A→G intergenic (+174990/+86367) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,334,5700AG96.0% 60.7 / NA 25intergenic (+174990/+86367)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (12/12);  total (12/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTAGATACTTTACTTAAGTTACCACAAGCAACAAGAGTTACCGCTGCGATTGCAGATAATAAGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAATTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAATCATCGCTGGTCATAATCTCATCCACTAA  >  1/1334350‑1334803
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
cttTAGATACTTTACTTAAGTTACCACAAGCAACAAGAGTTGCCGCTGCGATTGCAGATAATAAGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATa                                                                                                                                                                                                             >  1:114305/1‑251 (MQ=255)
 tttAGATACTTTACTTAAGTTACCACAAGCAACAAGAGTTGCCGCTGCGATTGCAGATAATAAGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAg                                                                                                                                                                                                            <  1:780165/251‑1 (MQ=255)
 tttAGATACTTTACTTAAGTTACCACAAGCAACAAGAGTTGCCGCTGCGATTGCAGATAATAAGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAg                                                                                                                                                                                                            <  1:691544/251‑1 (MQ=255)
                tAAGTTACCACAAGCAACAAGAGTTGCCGCTGCGATTGCAGATAATAAGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAg                                                                                                                                                                                                            >  1:866680/1‑236 (MQ=255)
                           aaGCAACAAGAGTTGCCGCTGCGATTGCAGATAATAAGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATc                                                                                                                                                                                  <  1:872490/251‑1 (MQ=255)
                                                            ataaGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAAt                                                                                                                                                                                                              >  1:99612/1‑190 (MQ=255)
                                                                                    gTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAg                                                                                                                                                                                                            <  1:77781/168‑1 (MQ=255)
                                                                                    gTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGt                                                                                                                                                    >  1:663293/1‑224 (MQ=255)
                                                                                              aTTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTaatc                                                                                                                                                                                                                           >  1:655606/1‑143 (MQ=255)
                                                                                                        gAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTaatc                                                                                                                                                                                                                           <  1:158878/133‑1 (MQ=255)
                                                                                                        gAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGTTGTTATATCAATGTTCTTTCATTTTTTTTAAGCCAACGTAAAGCAATATTATCGGCACTTTTTTCCGCCTGTTTACCGACtttttt                                                                                                     >  1:737566/1‑250 (MQ=255)
                                                                                                             gAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAg                                                                                                                                                                                                            >  1:880380/1‑143 (MQ=255)
                                                                                                                  aTCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACg                                                                                             <  1:561012/249‑1 (MQ=255)
                                                                                                                          gAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGAt                                                                                                                                                                                          <  1:824891/148‑1 (MQ=255)
                                                                                                                          gAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCa                                                                                    >  1:288813/1‑250 (MQ=255)
                                                                                                                           aaCCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCAc                                                                                   <  1:64488/250‑1 (MQ=255)
                                                                                                                                  gATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCAc                                                                                   >  1:611922/1‑243 (MQ=255)
                                                                                                                                                        aaaaGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAg                                                                                                                                                                                                            <  1:696123/100‑1 (MQ=255)
                                                                                                                                                                                   gCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAatcat                           >  1:222096/1‑250 (MQ=255)
                                                                                                                                                                                     tCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAATCATCGc                        <  1:297748/251‑1 (MQ=255)
                                                                                                                                                                                        gTATGGTAATTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAAcc                                                             >  1:570482/1‑211 (MQ=255)
                                                                                                                                                                                               aaTTACAAAACGGATTAAAGTAAATCCGAATTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAATCATCGCTGGTCATAAt              <  1:574510/251‑1 (MQ=255)
                                                                                                                                                                                                aTTACAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAATCATCGCTGGTCATAAtc             <  1:421770/251‑1 (MQ=255)
                                                                                                                                                                                                    cAAAACGGATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAATCATCGCTGGTCATAATCTCAt         <  1:461655/251‑1 (MQ=255)
                                                                                                                                                                                                           gATTAAAGTAAATCCGAGTTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAATCATCGCTGGTCATAATCTCATCCACtaa  >  1:875439/1‑251 (MQ=255)
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         
CTTTAGATACTTTACTTAAGTTACCACAAGCAACAAGAGTTACCGCTGCGATTGCAGATAATAAGAAATGTGATAATTTCATCTGTTTTACCTTATTTTTAGGTGAGTAGAACAATCCAAAGGAACCATAGATATAATAAAAAGAAGGCTCAAAAAGCAATAGTGCAACTTCAATTTCAGCTCCGTATGGTAATTACAAAACGGATTAAAGTAAATCCGAATTTTCTATTTTTAATCAATCTGTATAAATAGAATCGAAAAAAATAGGATGTTATATCAATGTTCTTTCATTTTTCTTAAGCCAACGTAAAGCAATATTATCGGCACTTTCTTCCGCCTGTTTACCGACTTTTTGTAATAACGATTTCTTCACCGCATATTTTACGCCGATAACCGATTTCTCTTTCATCGCCTGTAATAGCAAATCATCGCTGGTCATAATCTCATCCACTAA  >  1/1334350‑1334803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑