Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,328,775	T→C	intergenic (+169195/+92162)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,328,775	0	T	C	100.0%	137.2 / NA	48	intergenic (+169195/+92162)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

GTTGATTTTTGATGCGTTTTTTAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGTAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTACACCTTCAATTTCTTCGCTGGCGATGTTTTGA  >  1/1328564‑1329010                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            gTTGATTTTTGATGCGTTTTTTAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGc                                                                                                                                                                                                       >  1:25620/1‑250 (MQ=255)      tttttGATGCGTTTTTTAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCtttt                                                                                                                                                                                                  >  1:375951/1‑250 (MQ=255)      tttttGATGCGTTTTTTAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCtttt                                                                                                                                                                                                  >  1:171740/1‑250 (MQ=255)        tttGATGCGTTTTTTAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGt                                                                                                                                                                                               >  1:246662/1‑251 (MQ=255)            aTGCGTTTTTTAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGt                                                                                                                                                                                           <  1:180023/251‑1 (MQ=255)                 ttttttAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATcc                                                                                                                                                                                      <  1:740409/251‑1 (MQ=255)                 ttttttAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATc                                                                                                                                                                                       <  1:614610/250‑1 (MQ=255)                     ttAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAAc                                                                                                                                                                                  <  1:666200/251‑1 (MQ=255)                     ttAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAAc                                                                                                                                                                                  <  1:550755/251‑1 (MQ=255)                     ttAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAAc                                                                                                                                                                                  <  1:133911/251‑1 (MQ=255)                            gTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCa                                                                                                                                                                            >  1:306041/1‑250 (MQ=255)                            gTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCa                                                                                                                                                                            >  1:561528/1‑250 (MQ=255)                                            tttGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATc                                                                                                                                                           <  1:764292/251‑1 (MQ=255)                                                   gCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGAt                                                                                                                                                                                                                     >  1:41740/1‑186 (MQ=255)                                                      caTTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAAc                                                                                                                                                                                  >  1:134857/1‑218 (MQ=255)                                                        tttGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGcc                                                                                                                                                                        >  1:826957/1‑226 (MQ=255)                                                            ttgCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAAtc                                                                                                                                            <  1:474134/250‑1 (MQ=255)                                                                          cggTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTgttttggtt                                                                                                                             <  1:599202/251‑1 (MQ=255)                                                                            gTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTgttttggttt                                                                                                                            <  1:278563/250‑1 (MQ=255)                                                                                  tGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGat                                                                                                                     >  1:7127/1‑251 (MQ=255)                                                                                        aaaTAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAAt                                                                                                                                             >  1:317629/1‑221 (MQ=255)                                                                                               ggTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCa                                                                                                        >  1:776771/1‑251 (MQ=255)                                                                                                   aaTCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAgggg                                                                                                    <  1:557460/251‑1 (MQ=255)                                                                                                       ccTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATa                                                                                                >  1:116365/1‑251 (MQ=255)                                                                                                                 aCCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGc                                                                                        >  1:738679/1‑249 (MQ=255)                                                                                                                                 tATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGAttt                                                                      <  1:695091/251‑1 (MQ=255)                                                                                                                                        ttCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGa                                                               >  1:748938/1‑251 (MQ=255)                                                                                                                                        ttCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGa                                                               >  1:391/1‑251 (MQ=255)                                                                                                                                                          aCCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTgttttggtt                                                                                                                             >  1:128099/1‑171 (MQ=255)                                                                                                                                                           cccTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTAGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCCCGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTa                                            <  1:881450/251‑1 (MQ=255)                                                                                                                                                               gTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTgttt                                                                                                                                  <  1:170098/161‑1 (MQ=255)                                                                                                                                                               gTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGCCGATATAGGCAAAATc                                                                                                         >  1:439843/1‑186 (MQ=255)                                                                                                                                                               gTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTc                                                                                            <  1:888838/199‑1 (MQ=255)                                                                                                                                                                   cccTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGt                                                                                             >  1:160252/1‑194 (MQ=255)                                                                                                                                                                   cccTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGt                                    >  1:554768/1‑251 (MQ=255)                                                                                                                                                                   cccTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGt                                    >  1:482110/1‑251 (MQ=255)                                                                                                                                                                   cccTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGt                                    >  1:253462/1‑251 (MQ=255)                                                                                                                                                                       cAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGAt                                                                  <  1:155846/217‑1 (MQ=255)                                                                                                                                                                        aaaCTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAgga                               <  1:492655‑M1/251‑4 (MQ=255)                                                                                                                                                                        aaaCTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAgga                               >  1:485454‑M1/1‑248 (MQ=255)                                                                                                                                                                          aCTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaat                             <  1:865805‑M1/251‑6 (MQ=255)                                                                                                                                                                                ttttAAACACGACTTTCACCTCTTGTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaataacat                        <  1:858713‑M1/250‑11 (MQ=255)                                                                                                                                                                                         cGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaataacatcgcctttggt              <  1:605010‑M1/251‑21 (MQ=255)                                                                                                                                                                                         cGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaataacatcgcctttggt              <  1:230783‑M1/251‑21 (MQ=255)                                                                                                                                                                                         cGACTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaataacatcgcctttggt              <  1:94995‑M1/251‑21 (MQ=255)                                                                                                                                                                                           aCTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaataacatcgcctttggtt             <  1:75313‑M1/250‑22 (MQ=255)                                                                                                                                                                                           aCTTTCACCTCTTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaataacatcgcctttggtt             <  1:571232‑M1/250‑22 (MQ=255)                                                                                                                                                                                                     ctTTTGAGATTTGGGCAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTAggaataacatcgcctttggttttcgcatcagt  >  1:162789‑M1/1‑219 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            GTTGATTTTTGATGCGTTTTTTAAACGGTTCTGCCGCCGCGAATTTGTCGGCACATTTGTTGCATGTCGGCGGCGGTTTGATGGAAAAAATAGTGGTAAATCCCTCCAAACTACCCGGATAAAGAGGTTATCGTTTTCATAATCAACTCAGCCACCCTGTCCCCCTCAAACTGAGTTTTAAACACGACTTTCACCTCTTTTGAGATTTGGGTAAATAAACGTTCGGCGTGTTTGATTTTGCGTTCTTCGCCTTTTCGTAAGTTATCCGAACCATCCACGCCTTTTGCTTCAATCACAAAATTCAAAATCTCGCCTGTTTTGGTTTTGACGATATAGGCAAAATCAGGGGAATAAGTCCCGCCTCCTGCCACGGGGATTTTGATAGAATTTTTCGGGATTTTAGTAAACACGGTTACACCTTCAATTTCTTCGCTGGCGATGTTTTGA  >  1/1328564‑1329010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑