Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,324,726	T→G	intergenic (+165146/+96211)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,324,726	0	T	G	100.0%	83.0 / NA	30	intergenic (+165146/+96211)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/20);  total (10/20)

AATCGGGAAAACGTCCGATTTTGGTTTTGTCATAGCCGGTTTTTTCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATTGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAATTAATAATACATAATAAAATAATTTTGAATATGAAAACTTATGAAAGTTGTGATCAATATCACAAAACATTACAAATCGTATTTTCTAA  >  1/1324494‑1324933                                                                                                                                                                                                                                         |                                                                                                                                                                                                                aaTCGGGAAAACGTCCGATTTTGGTTTTGTCATAGCCGGTTTTTTCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAAtt                                                                                                                                                                                               >  1:140033/1‑251 (MQ=255)                cGATTTTGGTTTTGTCATAGCCGGTTTTTTCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGtat                                                                                                                                                                                <  1:759016/251‑1 (MQ=255)                     ttGGTTTTGTCATAGCCGGTTTTTTCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTTCCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAAttt                                                                                                                                                                           <  1:843616/251‑1 (MQ=255)                                     cGGTTTTTTCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGt                                                                                                                                                           <  1:856514/251‑1 (MQ=255)                                       gTTTTTTCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGttt                                                                                                                                                         >  1:848808/1‑251 (MQ=255)                                            ttCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAata                                                                                                                                                    <  1:667666/251‑1 (MQ=255)                                            ttCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAata                                                                                                                                                    <  1:520109/251‑1 (MQ=255)                                             tCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATTCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAatat                                                                                                                                                   <  1:581748/251‑1 (MQ=255)                                                     cATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACACAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCt                                                                                                                                           <  1:470796/251‑1 (MQ=255)                                                               ccGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGTCACTAACCTTTTACAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTCTAATTTTTCCAACCGTTTTTGTTTTAatat                                                                                                                                                   >  1:829444/1‑233 (MQ=255)                                                                       ctgctCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGat                                                                                                                         <  1:265059/251‑1 (MQ=255)                                                                                    cccGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAAc                                                                                                            <  1:123872/251‑1 (MQ=255)                                                                                    cccGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAAc                                                                                                            <  1:776800/251‑1 (MQ=255)                                                                                    cccGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAAc                                                                                                            <  1:758127/251‑1 (MQ=255)                                                                                    cccGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAAc                                                                                                            <  1:307733/251‑1 (MQ=255)                                                                                                     aaaTTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGat                                                                                                     >  1:124188/1‑241 (MQ=255)                                                                                                      aaTTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAAt                                                                                          <  1:644008/251‑1 (MQ=255)                                                                                                      aaTTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAAt                                                                                          <  1:830126/251‑1 (MQ=255)                                                                                                               tCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAATTAATAATAc                                                                                 <  1:38555/251‑1 (MQ=255)                                                                                                                                aGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAATTAATAATACAtaataa                                                                          <  1:544220/241‑1 (MQ=255)                                                                                                                                                              ccTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAgcg                                                                                                                                                                                                        >  1:525762/1‑85 (MQ=255)                                                                                                                                                                                ctgctCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAATTAATAATACATAATAAAATAATTTTGAATATGAAAACTTATGAAAGTTGTGATCAATATCACAAAACATTACaa                <  1:410683/251‑1 (MQ=255)                                                                                                                                                                                     cATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAAt                                                                                          <  1:108290/172‑1 (MQ=255)                                                                                                                                                                                             aCCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAATTAATAATACATAATAAAATAATTTTGAATATGAAAACTTATGAAAGTTGTGATCAATATCACAAAAc                       >  1:151380/1‑231 (MQ=255)                                                                                                                                                                                              ccTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAATTAATAATACATAATAAAATAATTTTGAATATGAAAACTTATGAAAGTTGTGATCAATATCACAAAACATTACAAATCGTATTTTCTaa  >  1:64221/1‑251 (MQ=255)                                                                                                                                                                                                    cACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAAc                                                                                                                                                                    <  1:385539/83‑1 (MQ=255)                                                                                                                                                                                                                gCTTTAACGCGCCGCGCTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGtt                                                                                                                                                          >  1:549080/1‑81 (MQ=255)                                                                                                                                                                                                                                cTGATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATc                                                                                                                                            >  1:139498/1‑79 (MQ=255)                                                                                                                                                                                                                                  gATCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAAt                                                                                          >  1:253797/1‑127 (MQ=255)                                                                                                                                                                                                                                    tCAATGGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAAt                                                                                          <  1:416760/125‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                AATCGGGAAAACGTCCGATTTTGGTTTTGTCATAGCCGGTTTTTTCGTAAGCCATAATTAAACCGCAATGCTGCTCTTTTAATCCCGCTGCCTGCCAGCCAAATTCCGGATCCAACAAAATGGCTGAAGCCCGTGTCGTCAAGTTCTCGGACACTAACCTTTTAAAAATCTGAAGCTGCTCATCCGTTACCTCTTCACCCAGCATCCGCTTTAACGCGCCGCGCTGATCAATTGCTAAAGCGCGAAAAATTCGCTCTTTACCGTATAATTTTTCCAACCGTTTTTGTTTTAATATTGTCATCTTTTTCTCCTACTTTCGATATTCACTTAAAACTTTCGATATGTTTTGAATTAATAATACATAATAAAATAATTTTGAATATGAAAACTTATGAAAGTTGTGATCAATATCACAAAACATTACAAATCGTATTTTCTAA  >  1/1324494‑1324933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑