Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,315,288	A→C	intergenic (+155708/+105649)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,315,288	0	A	C	100.0%	209.8 / NA	74	intergenic (+155708/+105649)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/40);  total (34/40)

ACGAACTACGCCATTTGGAAGAATTCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGAAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTATGGTAT  >  1/1315050‑1315523                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            aCGAACTACGCCATTTGGAAGAATTCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGAcc                                                                                                                                                                                                                                 <  1:293679/251‑1 (MQ=255) aCGAACTACGCCATTTGGAAGAATTCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGAc                                                                                                                                                                                                                                  <  1:379089/250‑1 (MQ=255) aCGAACTACGCCATTTGGAAGAATTCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGAc                                                                                                                                                                                                                                  <  1:100861/250‑1 (MQ=255)             aTTTGGAAGAATTCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATAc                                                                                                                                                                                                                      >  1:765333/1‑250 (MQ=255)                      aaTTCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAAt                                                                                                                                                                                                                        >  1:385070/1‑239 (MQ=255)                        ttCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          <  1:844567/251‑1 (MQ=255)                        ttCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          <  1:40978/251‑1 (MQ=255)                                   gcgcAGCGACGCGGCAAATTACAAAGCGTAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAAt                                                                                                                                                                                                                        <  1:103407/226‑1 (MQ=255)                                   gcgcAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATAc                                                                                                                                                                                                                      >  1:369302/1‑228 (MQ=255)                                   gcgcAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCg                                                                                                                                                                                                        >  1:675170/1‑242 (MQ=255)                                                gCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGAc                                                                                                                                                                                                                                  <  1:389555/203‑1 (MQ=255)                                                gCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          <  1:657708/227‑1 (MQ=255)                                                               cAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCCGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGt                                                                                                                                                                    <  1:689627/250‑1 (MQ=255)                                                                    cAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGAt                                                                                                                                                                                                                <  1:279049/201‑1 (MQ=255)                                                                    cAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTAcc                                                                                                                                                               >  1:371694/1‑250 (MQ=255)                                                                    cAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTAcc                                                                                                                                                               >  1:35190/1‑250 (MQ=255)                                                                     aaTAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCt                                                                                                                                                             <  1:736897/251‑1 (MQ=255)                                                                          cGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAg                                                                                                                                                        <  1:502460/251‑1 (MQ=255)                                                                          cGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAg                                                                                                                                                        <  1:734959/251‑1 (MQ=255)                                                                          cGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAg                                                                                                                                                        <  1:328899/251‑1 (MQ=255)                                                                                  ccTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGAc                                                                                                                                                                                            >  1:3703/1‑207 (MQ=255)                                                                                  ccTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAAt                                                                                                                                                >  1:619464/1‑251 (MQ=255)                                                                                                       gggCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTaa                                                                                                                           >  1:308564/1‑251 (MQ=255)                                                                                                            aTGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACggg                                                                                                                                        >  1:179068/1‑233 (MQ=255)                                                                                                                aaGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGc                                                                                                                                                                                                     >  1:118227/1‑168 (MQ=255)                                                                                                                  gCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          >  1:707864/1‑161 (MQ=255)                                                                                                                   ccATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAg                                                                                                                                                        >  1:165530/1‑210 (MQ=255)                                                                                                                          ggATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          <  1:276844/153‑1 (MQ=255)                                                                                                                          ggATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGa                                                                                                        >  1:254135/1‑251 (MQ=255)                                                                                                                            atatTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGAcc                                                                                                                                                                                                                                 >  1:421647/1‑128 (MQ=255)                                                                                                                            atatTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          <  1:573347/151‑1 (MQ=255)                                                                                                                            atatTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGc                                                                                                                                                                                  >  1:86572/1‑175 (MQ=255)                                                                                                                            atatTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGa                                                                                                                                              <  1:578745/211‑1 (MQ=255)                                                                                                                            atatTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTa                                                                                                                                 <  1:833459/224‑1 (MQ=255)                                                                                                                               ttattCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAGCACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGGCGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGaa                                                                                                                       <  1:178223/231‑1 (MQ=255)                                                                                                                               ttattCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAg                                                                                                    >  1:892669/1‑250 (MQ=255)                                                                                                                                 attCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTa                                                                                                                                 >  1:631547/1‑219 (MQ=255)                                                                                                                                 attCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGc                                                                                                   >  1:215020/1‑249 (MQ=255)                                                                                                                                      ggTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAAt                                                                                                                                                                                                                        >  1:466284/1‑127 (MQ=255)                                                                                                                                       gTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          >  1:781020/1‑140 (MQ=255)                                                                                                                                        tGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTCTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTg                                                                                                                                                                                                                           <  1:73065/122‑1 (MQ=255)                                                                                                                                             gATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCttttt                                                                                     >  1:323647/1‑251 (MQ=255)                                                                                                                                                cAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAatat                                                                                                                                                                                                          >  1:177552/1‑131 (MQ=255)                                                                                                                                                    gAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTatgatg                                                                              >  1:278242/1‑251 (MQ=255)                                                                                                                                                    gAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTatgatg                                                                              >  1:553314/1‑251 (MQ=255)                                                                                                                                                    gAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTatgatg                                                                              >  1:466363/1‑251 (MQ=255)                                                                                                                                                       atatTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCtt                                                                           >  1:105433/1‑251 (MQ=255)                                                                                                                                                        tatTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTa                                                                          >  1:6473/1‑251 (MQ=255)                                                                                                                                                           tCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTg                                                                       <  1:706549/251‑1 (MQ=255)                                                                                                                                                            cTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGa                                                                      <  1:659901/251‑1 (MQ=255)                                                                                                                                                              gATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAAt                                                                    >  1:404603/1‑251 (MQ=255)                                                                                                                                                                ttattTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGcc                                                                                                  >  1:2476/1‑219 (MQ=255)                                                                                                                                                                          cGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGc                                                             >  1:318509/1‑246 (MQ=255)                                                                                                                                                                              cacaCGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTaaa                                                    >  1:277606/1‑251 (MQ=255)                                                                                                                                                                                cacGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAgg                                                  <  1:147956/251‑1 (MQ=255)                                                                                                                                                                                 acGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGt                                                 <  1:879091/251‑1 (MQ=255)                                                                                                                                                                                      ccGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGAc                                                                                                                                                                                            >  1:222776/1‑107 (MQ=255)                                                                                                                                                                                      ccGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTaaaa                                            <  1:316376/251‑1 (MQ=255)                                                                                                                                                                                                      ctTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGttt                                                                                                                                                                  <  1:347736/117‑1 (MQ=255)                                                                                                                                                                                                          tCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTatgaaat                        <  1:321159/251‑1 (MQ=255)                                                                                                                                                                                                          tCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTatgaaat                        <  1:89532/251‑1 (MQ=255)                                                                                                                                                                                                           cAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTatgaaatg                       <  1:260861/251‑1 (MQ=255)                                                                                                                                                                                                               ggggTAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGt                                                                                                                                                                    <  1:659979/106‑1 (MQ=255)                                                                                                                                                                                                                   tAACCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAac               <  1:626748/251‑1 (MQ=255)                                                                                                                                                                                                                    aaCCTGGCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGaa                                                                                                                       <  1:583752/146‑1 (MQ=255)                                                                                                                                                                                                                         ggCAGGAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGt         >  1:94032/1‑251 (MQ=255)                                                                                                                                                                                                                              gAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTAt       <  1:785331/248‑1 (MQ=255)                                                                                                                                                                                                                              gAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTAt       <  1:80976/248‑1 (MQ=255)                                                                                                                                                                                                                              gAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTAt       <  1:92322/248‑1 (MQ=255)                                                                                                                                                                                                                              gAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTATgg     <  1:797766/250‑1 (MQ=255)                                                                                                                                                                                                                              gAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTATgg     <  1:389871/250‑1 (MQ=255)                                                                                                                                                                                                                              gAACACCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTATg      <  1:458248/249‑1 (MQ=255)                                                                                                                                                                                                                                acacCGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGATGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTATGGTAt  <  1:426715/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccGTAACGAGGGCAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAgg                                                  <  1:371769/200‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            ACGAACTACGCCATTTGGAAGAATTCCAACAAATGCGCAGCGACGCGGCAAATTACAAAGCGCAAAGCAATAACGGCGAGACCTTTGAGCAATTAGCCGAACGGGCAATGAAAGCCATACAGGATATTATTCAGGTGCATGATCAGGGAAATATTCTGATTATTTCCCACGGGCACACGCTCCGCTTGCTTTTATCTCTTTTCAACGGGGTAACCTGGCAGGAACACCGTAACGAGGGAAAAAGCCAGACCTTGCTGAATACCTCGATTAATATCGTGCGTTACCGACAAACCAACGCCGACGACGGTAAGTTTACCCTTGAAGTGATCAATGAAACGGGACATTTAAGTTAAAGAAAAAGCGCCAAGGTGATTAGCCTGCGGCGCTTTTTTATGATGCTTATTGAATGTTAGGCTTGGCTAAAGGTTAAAAACTCACCCACCGTATGAAATGAACCAAACACCAGTATGGTAT  >  1/1315050‑1315523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑