Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,314,556	A→C	intergenic (+154976/+106381)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,314,556	0	A	C	100.0%	197.8 / NA	72	intergenic (+154976/+106381)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/38);  total (34/38)

GCTCTGCGTGGCGTGGAGGTGTCCATTATTCTGCCGAAAGAAAACGATTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATACGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGATAATAATGAACAAAAATCTGACTTTTTATTTAATCCG  >  1/1314310‑1314792                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             gCTCTGCGTGGCGTGGAGGTGTCCATTATTCTGCCGAAAGAAAACGATTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCgg                                                                                                                                                                                                                                           >  1:899318/1‑250 (MQ=255) gCTCTGCGTGGCGTGGAGGTGTCCATTATTCTGCCGAAAGAAAACGATTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCgg                                                                                                                                                                                                                                           <  1:222298/250‑1 (MQ=255)         tggcgtggAGGTGTCCATTATTCTGCCGAAAGAAAACGATTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGcc                                                                                                                                                                                                                                   <  1:247577/250‑1 (MQ=255)                                 gCCGAAAGAAAACGATTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAg                                                                                                                                                                                                          >  1:595480/1‑251 (MQ=255)                                         aaaaCGATTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATc                                                                                                                                                                                                  <  1:573601/251‑1 (MQ=255)                                               aTTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATcaacaa                                                                                                                                                                                             <  1:231175/250‑1 (MQ=255)                                                     tgatgGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGaa                                                                                                                                                                                      >  1:731958/1‑251 (MQ=255)                                                       atgGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGa                                                                                                                                                                                                            >  1:565489/1‑227 (MQ=255)                                                       atgGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAAt                                                                                                                                                                                     <  1:724918/250‑1 (MQ=255)                                                               cTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATc                                                                                                                                                                            <  1:149080/251‑1 (MQ=255)                                                                    cAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGcc                                                                                                                                                                       <  1:406409/251‑1 (MQ=255)                                                                       gTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATc                                                                                                                                                                            >  1:786456/1‑243 (MQ=255)                                                                       gTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAg                                                                                                                                                                    >  1:116025/1‑251 (MQ=255)                                                                       gTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAg                                                                                                                                                                    >  1:837601/1‑251 (MQ=255)                                                                        tCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGt                                                                                                                                                                   <  1:215512/251‑1 (MQ=255)                                                                                  tttGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCg                                                                                                                                                         <  1:621098/251‑1 (MQ=255)                                                                                      atgatTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATc                                                                                                                                                                            >  1:546328/1‑228 (MQ=255)                                                                                        gatTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAAt                                                                                                                                                                                     <  1:463168/217‑1 (MQ=255)                                                                                              cTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCa                                                                                                                                                                           >  1:90322/1‑221 (MQ=255)                                                                                                                                   ggTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCg                                                                                                        >  1:209696/1‑251 (MQ=255)                                                                                                                                    gTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCa                                                                                                                                        >  1:731539/1‑218 (MQ=255)                                                                                                                                          gCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCtgttgt                                                                                                 >  1:788343/1‑251 (MQ=255)                                                                                                                                              acTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTgtagta                                                                                             <  1:465322/251‑1 (MQ=255)                                                                                                                                              acTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTgtagta                                                                                             <  1:734350/251‑1 (MQ=255)                                                                                                                                               cTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATcaac                                                                                                                                                                                               <  1:714274/152‑1 (MQ=255)                                                                                                                                               cTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAAt                                                                                                                                                                                     >  1:773079/1‑162 (MQ=255)                                                                                                                                               cTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTaa                                                                                            >  1:634334/1‑251 (MQ=255)                                                                                                                                               cTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTaa                                                                                            >  1:175461/1‑251 (MQ=255)                                                                                                                                               cTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATAAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTaa                                                                                            >  1:521557/1‑251 (MQ=255)                                                                                                                                                 aaaaGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTaaaa                                                                                          <  1:499328/251‑1 (MQ=255)                                                                                                                                                 aaaaGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGATTTTTATTGAATTTTGAAGTGACGATGGTGGTGGAAGACCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTaaaa                                                                                          <  1:580854/251‑1 (MQ=255)                                                                                                                                                    aGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGt                                                                                       >  1:208020/1‑251 (MQ=255)                                                                                                                                                            cATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATc                                                                                                                                                                            >  1:420592/1‑158 (MQ=255)                                                                                                                                                            cATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCaa                                                                                                                                            <  1:66474/190‑1 (MQ=255)                                                                                                                                                              ttGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTc                                                                                                              >  1:162916/1‑218 (MQ=255)                                                                                                                                                                 aTAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTtc                                                                          <  1:718782/251‑1 (MQ=255)                                                                                                                                                                 aTAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTtc                                                                          <  1:516813/251‑1 (MQ=255)                                                                                                                                                                  tAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAAt                                                                                                                                                           <  1:543458/169‑1 (MQ=255)                                                                                                                                                                  tAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGAtttt                                                                           >  1:457063/1‑249 (MQ=255)                                                                                                                                                                               ttGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTAtc                                                            <  1:38673/251‑1 (MQ=255)                                                                                                                                                                                 ggTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTTCCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTAtct                                                           >  1:63016/1‑250 (MQ=255)                                                                                                                                                                                      gCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGcc                                                                                                                                                                                                                                   <  1:410107/77‑1 (MQ=255)                                                                                                                                                                                      gCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAAt                                                                                                                                                                                     >  1:433957/1‑123 (MQ=255)                                                                                                                                                                                         ccGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATTGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACAt                                                  <  1:410978/251‑1 (MQ=255)                                                                                                                                                                                         ccGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACAt                                                  <  1:315160/251‑1 (MQ=255)                                                                                                                                                                                           gTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATcaac                                                                                                                                                                                               >  1:858195/1‑108 (MQ=255)                                                                                                                                                                                             tAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATc                                                                                                                                                                                                  >  1:218792/1‑103 (MQ=255)                                                                                                                                                                                               aCATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAGTTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAAt                                             >  1:449920/1‑250 (MQ=255)                                                                                                                                                                                               aCATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAAt                                             >  1:562400/1‑250 (MQ=255)                                                                                                                                                                                               aCATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAAt                                             >  1:819555/1‑250 (MQ=255)                                                                                                                                                                                                cATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTaaaa                                                                                          <  1:213637/204‑1 (MQ=255)                                                                                                                                                                                                cATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATc                                            <  1:459640/250‑1 (MQ=255)                                                                                                                                                                                                   ggATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTaaaa                                                                                          <  1:96661/201‑1 (MQ=255)                                                                                                                                                                                                   ggATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGGGCGGTTGATTTTCTCGGGGTTTTTTTCTCGTTACCATTTATTCAAgg                                        >  1:778915/1‑251 (MQ=255)                                                                                                                                                                                                     atatGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGat                                      <  1:307421/251‑1 (MQ=255)                                                                                                                                                                                                          cgcAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGAtaataa                                 <  1:553124/251‑1 (MQ=255)                                                                                                                                                                                                          cgcAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGAtaataa                                 <  1:185702/251‑1 (MQ=255)                                                                                                                                                                                                          cgcAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGAtaataa                                 <  1:834312/251‑1 (MQ=255)                                                                                                                                                                                                           caggCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGAtaataa                                 <  1:422567/247‑1 (MQ=255)                                                                                                                                                                                                              gCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGATAATAATGaa                             >  1:791680/1‑251 (MQ=255)                                                                                                                                                                                                                            tttGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTAtct                                                           >  1:718209/1‑207 (MQ=255)                                                                                                                                                                                                                            tttGAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACAt                                                  <  1:862495/216‑1 (MQ=255)                                                                                                                                                                                                                               gAAGTGACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGATAATAATGAACAAAAATCTGACttt              <  1:743794/249‑1 (MQ=255)                                                                                                                                                                                                                                    gACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAg                                                                                                                                                                                                          >  1:715410/1‑56 (MQ=255)                                                                                                                                                                                                                                    gACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCaa                                                                                                                                            <  1:172441/118‑1 (MQ=255)                                                                                                                                                                                                                                    gACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTa                                                              <  1:308319/196‑1 (MQ=255)                                                                                                                                                                                                                                    gACTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGataat                                   <  1:49074/223‑1 (MQ=255)                                                                                                                                                                                                                                      cTATGGTGGTGGAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGATAATAATGAACAAAAATCTGACTTTTTATTTAAt     >  1:707344/1‑251 (MQ=255)                                                                                                                                                                                                                                         tggtggtggAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCg                                                                                                                                                         >  1:520206/1‑100 (MQ=255)                                                                                                                                                                                                                                          ggtggtggAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGATAATAATGAACAAAAATCTGACTTTTTATTTAATCCg  >  1:162286/1‑250 (MQ=255)                                                                                                                                                                                                                                           gtggtggAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATc                                                                                                                                              <  1:379409/109‑1 (MQ=255)                                                                                                                                                                                                                                             ggtggAAGATCCGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATc                                            <  1:818801/205‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             GCTCTGCGTGGCGTGGAGGTGTCCATTATTCTGCCGAAAGAAAACGATTCCATGATGGTACGCTGGGCAAGTCGTACCTTTTTTGATGATTTACTTGCCGCCGGCGTAAAAATTTATAATTTCAAGAAAGGGTTATTGCACACTAAAAGTATGCTCATTGATAACAAGCTCGCTTTGGTCGGCACCGTTAACATGGATATGCGCAGCTTTTTATTGAATTTTGAAGTGACTATGGTGGTGGAAGATACGGCGTTCGCCAATGAAATTTCCTTGCTCCACGAAGGTTACATCAACAATTCAGAATTACCTGATCATGCCAAGTGGGCAAATCGTTCCGTTTATCAACGCATTATCGAAAAATTGTTTTTCCTCTTCAGCCCGCTGTTGTAGTAAAAAGTGCGGTTGATTTTCTCGGTGTTTTTATCTCGATAACATTTAATCAAGGATAATAATGAACAAAAATCTGACTTTTTATTTAATCCG  >  1/1314310‑1314792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑