Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,311,897	T→C	intergenic (+152317/+109040)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,311,897	0	T	C	100.0%	197.9 / NA	72	intergenic (+152317/+109040)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/32);  total (40/32)

TGATAAGCGTGCAGATAGGTGCGATCGGCGCTATCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTTTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCATGGAAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATACCGTTCGGTTTGT  >  1/1311651‑1312143                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tGATAAGCGTGCAGATAGGTGCGATCGGCGCTATCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTccc                                                                                                                                                                                                                                                    <  1:103409/251‑1 (MQ=255)             aGATAGGTGCGATCGGCGCTATCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCtt                                                                                                                                                                                                                                        >  1:265772/1‑251 (MQ=255)                  ggTGCGATCGGCGCTATCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTCGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGtt                                                                                                                                                                                                                                   >  1:246205/1‑251 (MQ=255)                      cGATCGGCGCTATCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTtgtg                                                                                                                                                                                                                               <  1:561345/251‑1 (MQ=255)                            gcgcTATCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTTAGCTg                                                                                                                                                                                                                          >  1:219825/1‑250 (MQ=255)                                 aTCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTg                                                                                                                                                                                                                    >  1:525337/1‑251 (MQ=255)                                          aTACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAg                                                                                                                                                                                                           >  1:653281/1‑251 (MQ=255)                                          aTACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAg                                                                                                                                                                                                           >  1:63028/1‑251 (MQ=255)                                          aTACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAg                                                                                                                                                                                                           >  1:660279/1‑251 (MQ=255)                                          aTACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAg                                                                                                                                                                                                           >  1:516295/1‑251 (MQ=255)                                          aTACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAACACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAg                                                                                                                                                                                                           >  1:447769/1‑251 (MQ=255)                                            aCAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGtt                                                                                                                                                                                                         <  1:690762/251‑1 (MQ=255)                                                  cATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGa                                                                                                                                                                                                   <  1:891304/251‑1 (MQ=255)                                                                ggACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATg                                                                                                                                                                                      >  1:878745/1‑250 (MQ=255)                                                                ggACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATg                                                                                                                                                                                      >  1:277298/1‑250 (MQ=255)                                                                     gCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGTGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCaaa                                                                                                                                                                                  >  1:215933/1‑249 (MQ=255)                                                                     gCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGt                                                                                                                                                                                                          >  1:396341/1‑225 (MQ=255)                                                                     gCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCaaa                                                                                                                                                                                  >  1:880926/1‑249 (MQ=255)                                                                             ttttCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGAc                                                                                                                                                                        <  1:317412/251‑1 (MQ=255)                                                                                        acacGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCtt                                                                                                                                                                                                                                        <  1:423548/176‑1 (MQ=255)                                                                                        acacGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacgg                                                                                                                                                              >  1:785564/1‑247 (MQ=255)                                                                                        acacGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacgg                                                                                                                                                              >  1:5982/1‑247 (MQ=255)                                                                                        acacGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacgg                                                                                                                                                              >  1:412684/1‑247 (MQ=255)                                                                                        acacGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacgg                                                                                                                                                              <  1:152688/250‑4 (MQ=255)                                                                                             gAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacggcagc                                                                                                                                                          >  1:618997/1‑242 (MQ=255)                                                                                               gCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacggcagc                                                                                                                                                          >  1:445220/1‑240 (MQ=255)                                                                                                       gTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTa                                                                                                                                               <  1:80352/250‑1 (MQ=255)                                                                                                         tttGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATg                                                                                                                                            <  1:552416/251‑1 (MQ=255)                                                                                                                gTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACa                                                                                                                                      <  1:330863/250‑1 (MQ=255)                                                                                                                gTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACa                                                                                                                                      <  1:803892/250‑1 (MQ=255)                                                                                                                     cGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTa                                                                                                                                               <  1:198196/236‑1 (MQ=255)                                                                                                                       gTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGAc                                                                                                                                                                        <  1:213212/209‑1 (MQ=255)                                                                                                                       gTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATtaataa                                                                                                                               <  1:832837/250‑1 (MQ=255)                                                                                                                           cAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCg                                                                                                                          >  1:718292/1‑251 (MQ=255)                                                                                                                             gAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAg                                                                                                                        >  1:466888/1‑251 (MQ=255)                                                                                                                                 aaataaGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacggc                                                                                                                                                             >  1:623956/1‑206 (MQ=255)                                                                                                                                       gcgcAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTAt                                                                                                              >  1:70784/1‑251 (MQ=255)                                                                                                                                          cAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATcc                                                                                                            <  1:610557/250‑1 (MQ=255)                                                                                                                                            aaTTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAAccc                                                                                                                            <  1:720596/232‑1 (MQ=255)                                                                                                                                                  gTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACttt                                                                                                                <  1:538789/238‑1 (MQ=255)                                                                                                                                                  gTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGa                                                                                                   >  1:397366/1‑251 (MQ=255)                                                                                                                                                     cGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAAcc                                                                                                <  1:886653/251‑1 (MQ=255)                                                                                                                                                            aTGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGTTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCacggc                                                                                                                                                             >  1:558887/1‑179 (MQ=255)                                                                                                                                                            aTGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCaaa                                                                                         >  1:463963/1‑251 (MQ=255)                                                                                                                                                                 acacAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTg                                                                                     >  1:188303/1‑250 (MQ=255)                                                                                                                                                                  cacaAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCg                                                                                   >  1:345946/1‑251 (MQ=255)                                                                                                                                                                                cGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGTATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGt                                                                     <  1:264110/251‑1 (MQ=255)                                                                                                                                                                                                gctttgGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACCTTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCggg                                                     >  1:434232/1‑251 (MQ=255)                                                                                                                                                                                                  tttgGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGtt                                                   <  1:611783/251‑1 (MQ=255)                                                                                                                                                                                                      ggTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAg                                                >  1:701688/1‑250 (MQ=255)                                                                                                                                                                                                        tCAGTTTCCAAGCTCCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGcc                                              <  1:901806/250‑1 (MQ=255)                                                                                                                                                                                                        tCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGcc                                              <  1:389970/250‑1 (MQ=255)                                                                                                                                                                                                        tCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCg                                             >  1:622489/1‑251 (MQ=255)                                                                                                                                                                                                          cgTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGAt                                           >  1:170240/2‑251 (MQ=255)                                                                                                                                                                                                           gTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCt                                                                                                                                          <  1:30242/155‑1 (MQ=255)                                                                                                                                                                                                           gTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCgg                                                                                  >  1:377387/1‑211 (MQ=255)                                                                                                                                                                                                           gTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATg                                          >  1:654692/1‑251 (MQ=255)                                                                                                                                                                                                              tCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCg                                       >  1:193893/1‑251 (MQ=255)                                                                                                                                                                                                                      ccTCGGCGCGCTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATtaataa                                                                                                                               >  1:20452/1‑155 (MQ=255)                                                                                                                                                                                                                            cgcgcTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTtgtg                         <  1:666593/251‑1 (MQ=255)                                                                                                                                                                                                                            cgcgcTTTTTGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTtgtg                         <  1:272409/251‑1 (MQ=255)                                                                                                                                                                                                                                   tttGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTg                  <  1:832897/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttGCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGcc                                              <  1:86722/222‑1 (MQ=255)                                                                                                                                                                                                                                      gCATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATtaataa                                                                                                                               >  1:518461/1‑139 (MQ=255)                                                                                                                                                                                                                                       cATATCCCCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATAc              <  1:598606/251‑1 (MQ=255)                                                                                                                                                                                                                                       cATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATAc              <  1:787115/251‑1 (MQ=255)                                                                                                                                                                                                                                       cATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATAc              <  1:391302/251‑1 (MQ=255)                                                                                                                                                                                                                                       cATATCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATAc              <  1:467919/251‑1 (MQ=255)                                                                                                                                                                                                                                         tatCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTa                                                                                                                                               >  1:129766/1‑120 (MQ=255)                                                                                                                                                                                                                                         tatCACCGCTGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCG‑TGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATACCGt           >  1:162711/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tGATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATtaat                                                                                                                                 <  1:540357/125‑1 (MQ=255)                                                                                                                                                                                                                                                   gATTCTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCACGGCAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATACCGTTCGGTTTGt  >  1:744313/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TGATAAGCGTGCAGATAGGTGCGATCGGCGCTATCGCCGCCATACAAAGCATCGCCTAAAATCGGACTGCCTAAACTTTTCATAGCGACACGGAGCTGATGGGTTTTGTCGGTTTTCGGTTGCAGAATAAATAAGCGCAAATTTGGTGCGAGACTATGGGACACAAAATCTGTCACGGCAGGATTTTCTTTGCTTTGGGTCAGTTTCCAAGCACCTCGGCGCGCTTTTTGCATATCACCGCTGATTTTCCCCTGCTTTTTCTTTGGTTTGTGAGCTGCCAGTGCGAGGTAAGTTTTCTGAATTTGATGATATGCAAACTTTTGTGACAACGTCATGGAAGCCTGTTCATTTAATGCTAACATTAATAACCCCGAGGTCACTTTATCCAGCCGATGAACCAGCCAAACCTGCGGCACGCCAAGCTGTTGCGCCACCAGTCGGGTTAAGCCGATGTCGGCATCGTCTTTGTGTACGCTGATACCGTTCGGTTTGT  >  1/1311651‑1312143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑