Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,308,578	A→G	intergenic (+148998/+112359)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,308,578	0	A	G	100.0%	92.0 / NA	33	intergenic (+148998/+112359)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

AAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAAAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATAAAGAAAGCATTTTCATCTATTATAAGTAACATTGACTTCCCAAAACTAGAAGCCAAAGGTTTTAGTCTTTCAGTTACTCAAAGTATGATAAACAGCATAATAT  >  1/1308336‑1308816                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               aaaCATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCataa                                                                                                                                                                                                                                        >  1:184885/1‑251 (MQ=255) aaaCATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCataa                                                                                                                                                                                                                                        <  1:773902/251‑1 (MQ=255)        acattGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAAttt                                                                                                                                                                                                                                  >  1:311202/1‑250 (MQ=255)                                tCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGGTCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATg                                                                                                                                                                                                           <  1:103011/249‑1 (MQ=255)                                tCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATg                                                                                                                                                                                                           <  1:114073/249‑1 (MQ=255)                                      ttGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTa                                                                                                                                                                                                   <  1:516325/251‑1 (MQ=255)                                            ccctccACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGt                                                                                                                                                                                             <  1:527454/251‑1 (MQ=255)                                                            aTGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGGAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAAt                                                                                                                                                                             <  1:821184/251‑1 (MQ=255)                                                                               tCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTg                                                                                                                                                           >  1:169220/1‑250 (MQ=255)                                                                                  gAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCtat                                                                                                                                                       >  1:391973/1‑251 (MQ=255)                                                                                       gagaATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATaataat                                                                                                                                                                                                                                    >  1:677812/1‑169 (MQ=255)                                                                                       gagaATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTa                                                                                                                                                                                                             >  1:828964/1‑192 (MQ=255)                                                                                       gagaATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAAt                                                                                                                                                                             >  1:162474/1‑224 (MQ=255)                                                                                       gagaATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAac                                                                                                                                                   <  1:737754/250‑1 (MQ=255)                                                                                               aTCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGAt                                                                                                                                                                                       <  1:382603/206‑1 (MQ=255)                                                                                                 cATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCataa                                                                                                                                                                                                                                        <  1:879286/155‑1 (MQ=255)                                                                                                 cATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTAt                                                                                                                                                                                           >  1:641552/1‑200 (MQ=255)                                                                                                  aTTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGAt                                                                                                                                                                                       >  1:845244/1‑203 (MQ=255)                                                                                                                 ttATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTGTGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGat                                                                                                                        <  1:545540/251‑1 (MQ=255)                                                                                                                            actGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTa                                                                                                                                                                                                             >  1:464768/1‑155 (MQ=255)                                                                                                                            actGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACt                                                                                                                                                <  1:553330/216‑1 (MQ=255)                                                                                                                                   aaaTTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATaaag                                                                                                      <  1:558871/251‑1 (MQ=255)                                                                                                                                                  tattaAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGttt                                                                                                                                                                                                        <  1:870926/138‑1 (MQ=255)                                                                                                                                                      aaaCCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATAAAGAAAGCATTTTCATCtatta                                                                                   <  1:158180/251‑1 (MQ=255)                                                                                                                                                                aatcaaGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAAt                                                                                                                                                                             <  1:488062/151‑1 (MQ=255)                                                                                                                                                                 atcaaGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGattat                                                                                                                     >  1:729162/1‑206 (MQ=255)                                                                                                                                                                                     aTTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTc                                                                                                                                                                                     >  1:761475/1‑122 (MQ=255)                                                                                                                                                                                        gCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTa                                                                                                                                                                                                                   >  1:105935/1‑89 (MQ=255)                                                                                                                                                                                          aCTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATAAAGAAAGCATTTTCATCTATTATAAGTAACATTGACTTCCCAAAACTAGAAGCCAAAg                                               >  1:416036/1‑251 (MQ=255)                                                                                                                                                                                               cAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATCGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATAAAGAAAGCATTTTCATCTATTATAAGTAACATTGACTTCCCAAAACTAGAAGCCAAAGGtttt                                          >  1:687239/1‑251 (MQ=255)                                                                                                                                                                                                 ggTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGaaaaa                                                                                                                            <  1:207358/167‑1 (MQ=255)                                                                                                                                                                                                                           tttCTGGAAGAATTTCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATAAAGAAAGCATTTTCATCTATTATAAGTAACATTGACTTCCCAAAACTAGAAGCCAAAGGTTTTAGTCTTTCAGTTACTCAAAGTATGATaa              <  1:845713/251‑1 (MQ=255)                                                                                                                                                                                                                                       tttCTATGAAAAGAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATAAAGAAAGCATTTTCATCTATTATAAGTAACATTGACTTCCCAAAACTAGAAGCCAAAGGTTTTAGTCTTTCAGTTACTCAAAGTATGATAAACAGCATAatat  <  1:471045/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               AAACATTACATTGCATTCGCTTTCAGTTAAGTCTGGATTGGCTCCCTCCACATTAAAAAATGCATTGCGAGTTAGTTATCCGAAGGGAGAATGGATCATTTAGAGTAACCAATTATGGCAACTACTGAAAAAATTGAAGAAAAATTATTAAACCTTATCAATCAAGCAGACTCTTTTGCTATTGCACTTACAGGTGAATGGGGAATTGGTAAAACGCATTTCTGGAAGAATTTCTATGAAAAAAATCATAATAATTTCAAAATGAATAAGTATTCTTATGTTTCGTTATTTGGTATTGATTCCTTAGAATCATTTAAATATCAAATTGCTATAAACACTCATGATACAAAGCAGAAAAAAGATTATTTATTACATATAAAGAAAGCATTTTCATCTATTATAAGTAACATTGACTTCCCAAAACTAGAAGCCAAAGGTTTTAGTCTTTCAGTTACTCAAAGTATGATAAACAGCATAATAT  >  1/1308336‑1308816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑