Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,308,096	C→A	intergenic (+148516/+112841)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,308,096	0	C	A	100.0%	41.4 / NA	17	intergenic (+148516/+112841)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (5/12);  total (5/12)

AAAGCCTAGGGCGCGCTGCACCTCGTAAGGCATGGATAAATAATGGTATTCGTATGTTTTCCCTTTTCTTCCTTTTATAAGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTCTTAACGTATTGATTTAAACAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTGGTTTTAAACTTAGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAG  >  1/1307851‑1308333                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              aaaGCCTAGGGCGCGCTGCACCTCGTAAGGCATGGATAAATAATGGTATTCGTATGTTTTCCCTTTTCTTCCTTTTATAAGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAAc                                                                                                                                                                                                                                          <  1:902023/251‑1 (MQ=255)     ccTAGGGCGCGCTGCACCTCGTAAGGCATGGATAAATAATGGTATTCGTATGTTTTCCCTTTTCTTCCTTTTATAAGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGt                                                                                                                                                                                                                                        <  1:256132/249‑1 (MQ=255)                                            tGGTATTCGTATGTTTTCCCTTTTCTTCCTTTTATAAGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTAc                                                                                                                                                                                               <  1:543137/251‑1 (MQ=255)                                                        gTTTTCCCTTTTCTTCCTTTTATAAGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAAc                                                                                                                                                                                                                                          >  1:239984/1‑196 (MQ=255)                                                                             ataAGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACtttagg                                                                                                                                                              <  1:279325/251‑4 (MQ=255)                                                                               aaGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtt                                                                                                                                                            <  1:486843‑M1/251‑4 (MQ=255)                                                                                      cTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaacta                                                                                                                                                     >  1:117822‑M1/1‑241 (MQ=255)                                                                                                       tttGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaagggg                                                                                                                                    <  1:101781‑M1/251‑28 (MQ=255)                                                                                                             ttttttAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaaggggtaagac                                                                                                                              <  1:299749‑M1/251‑34 (MQ=255)                                                                                                                                   gTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTGATTTACAATACCTATTAACg                                                                                                                                                                                                                                         >  1:249824/1‑122 (MQ=255)                                                                                                                                   gTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTAttgttg                                                                                                                                                                      <  1:553724/189‑1 (MQ=255)                                                                                                                                                          cAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaaggggtaagactaaagatttacacccaagtttaacctaatctttaaattattcaaa                                                                                 <  1:364573‑M1/251‑79 (MQ=255)                                                                                                                                                                                  ctctCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaaggggtaagactaaagatttacacccaagtttaacctaatctttaaattattcaaatgcaaaagcgaggaactatga                                                            >  1:258807‑M1/1‑149 (MQ=255)                                                                                                                                                                                                           gTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaaggggtaagactaaagatttacacccaagtttaacctaatctttaaattattcaaatgcaaaagcgaggaactatgaaaaaaaaggattggagtagaaagcggat                                >  1:142910‑M1/1‑124 (MQ=255)                                                                                                                                                                                                                             aaaCTAAGTTTAATTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaaggggtaagac                                                                                                                              <  1:900553‑M1/139‑34 (MQ=255)                                                                                                                                                                                                                                         aTTTACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaaggggtaagactaaagatttacacccaagtttaacctaatctttaaattattcaaatgcaaaagcgaggaactatgaaaaaaaaggattggagtagaaagcggattgtttacgagttacatgagcgaaacattac  <  1:741275‑M1/251‑158 (MQ=255)                                                                                                                                                                                                                                          tttACAATACCTATTAACGTATTGATTTAAATAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTAGgtttaaactaagtttaacgctaaggggtaagac                                                                                                                              <  1:174772‑M1/126‑34 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              AAAGCCTAGGGCGCGCTGCACCTCGTAAGGCATGGATAAATAATGGTATTCGTATGTTTTCCCTTTTCTTCCTTTTATAAGTCGTCTTTCCCAGTTTTCTAATTTGGCTTTTTTAGTTACCCCTTGAACAGTTTCTGGTAGTCCAGCTACTCCCAAAAGGTCTTTCAGTTCTAGCCACTCTCGGATCATATCTAACCTCCTAGTTTTAAACCAAGGATTAAAACTAAGTTTAATTTACAATACCTCTTAACGTATTGATTTAAACAAAAATAAATTTCTTTACATAAGTTTTACAAAATTTTTATTTAAACTATTGTTGACTTTGGTTTTAAACTTAGTTTAAACTAAGTTTAACGCTAAGGGGTAAGACTAAAGATTTACACCCAAGTTTAACCTAATCTTTAAATTATTCAAATGCAAAAGCGAGGAACTATGAAAAAAAAGGATTGGAGTAGAAAGCGGATTGTTTACGAGTTACATGAG  >  1/1307851‑1308333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑