Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	290,145	C→A	intergenic (‑22785/+54460)	unknown ← / ← unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	290,145	0	C	A	100.0%	177.8 / NA	66	intergenic (‑22785/+54460)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/26);  total (40/26)

GTTATAACCATCAGACGTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCCGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAGGAGCGATAGATACACGTCCACCTA  >  1/289904‑290389                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     gTTATAACCATCAGACGTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAAtct                                                                                                                                                                                                                                              >  1:79100/1‑250 (MQ=255)     taACCATCAGTCGTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTGCAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAGCGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAAtt                                                                                                                                                                                                                                         <  1:78919/251‑1 (MQ=255)           tCAGACGTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTAc                                                                                                                                                                                                                                   <  1:408949/251‑1 (MQ=255)           tCAGACGTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTAc                                                                                                                                                                                                                                   <  1:68202/251‑1 (MQ=255)              gACGTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCa                                                                                                                                                                                                                                                  >  1:869675/1‑233 (MQ=255)                 gTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAAttt                                                                                                                                                                                                                                        >  1:628949/1‑240 (MQ=255)                   cTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTtctct                                                                                                                                                                                                                            >  1:39861/1‑250 (MQ=255)                      tGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTcccc                                                                                                                                                                                                                        <  1:574296/251‑1 (MQ=255)                         gaCTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAAttt                                                                                                                                                                                                                                        >  1:158484/1‑232 (MQ=255)                             gTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATttatt                                                                                                                                                                                                                                     >  1:228178/1‑231 (MQ=255)                             gTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCt                                                                                                                                                                                                                       >  1:704795/1‑245 (MQ=255)                             gTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTg                                                                                                                                                                                                                 >  1:7673/1‑251 (MQ=255)                                        ttttAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAataaataa                                                                                                                                                                                                      >  1:363661/1‑251 (MQ=255)                                                cAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCaat                                                                                                                                                                                              <  1:339315/251‑1 (MQ=255)                                                      aCGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGaaa                                                                                                                                                                                                             >  1:676005/1‑230 (MQ=255)                                                                  cTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGt                                                                                                                                                                                         >  1:392912/1‑238 (MQ=255)                                                                         aTCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGa                                                                                                                                                                     <  1:361201/251‑1 (MQ=255)                                                                          tCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGAc                                                                                                                                                                    <  1:777025/251‑1 (MQ=255)                                                                                   aTTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTgg                                                                                                                                                                                                                >  1:201752/1‑198 (MQ=255)                                                                                    tttttCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAAt                                                                                                                                                          >  1:747728/1‑251 (MQ=255)                                                                                                   ttCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGaaataaat                                                                                                                                                                                                        <  1:104689/190‑1 (MQ=255)                                                                                                   ttCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCaataat                                                                                                                                                                                           >  1:490128/1‑203 (MQ=255)                                                                                                   ttCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTg                                                                                                                                                                                   >  1:4815/1‑211 (MQ=255)                                                                                                   ttCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGtt                                                                                                                                            >  1:229972/1‑250 (MQ=255)                                                                                                   ttCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGtt                                                                                                                                            >  1:116322/1‑250 (MQ=255)                                                                                                     cTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCaa                                                                                                                                         >  1:782251/1‑251 (MQ=255)                                                                                                        ggATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGaaa                                                                                                                                                                                                             <  1:279995/180‑1 (MQ=255)                                                                                                                   ccGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGaaataaat                                                                                                                                                                                                        >  1:497947/1‑174 (MQ=255)                                                                                                                   ccGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCaaaa                                                                                                                           <  1:106234/251‑1 (MQ=255)                                                                                                                               tATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAg                                                                                                               <  1:305576/251‑1 (MQ=255)                                                                                                                                 tCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTc                                                                                                                                                                                                                     <  1:450039/147‑1 (MQ=255)                                                                                                                                 tCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCg                                                                                                             >  1:711733/1‑251 (MQ=255)                                                                                                                                                  tCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAg                                                                                            <  1:463523/251‑1 (MQ=255)                                                                                                                                                     aTAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTAccc                                                                                                   <  1:561026/241‑1 (MQ=255)                                                                                                                                                     aTAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAg                                                                                            >  1:338378/1‑248 (MQ=255)                                                                                                                                                             cTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTAccc                                                                                                   <  1:804075/233‑1 (MQ=255)                                                                                                                                                               ccTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCGGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAaataat                                                                                                                                                                                                     >  1:337958/1‑133 (MQ=255)                                                                                                                                                               ccTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTAc                                                                                                                                                                                                                                   >  1:569766/1‑103 (MQ=255)                                                                                                                                                               ccTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAg                                                                                            <  1:622798/238‑1 (MQ=255)                                                                                                                                                               ccTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAgg                                                                               >  1:488045/1‑251 (MQ=255)                                                                                                                                                               ccTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAgg                                                                               >  1:211974/1‑251 (MQ=255)                                                                                                                                                               ccTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAgg                                                                               >  1:520481/1‑251 (MQ=255)                                                                                                                                                                 taataaGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGc                                                                             <  1:362826/251‑1 (MQ=255)                                                                                                                                                                      aGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCt                                                                            >  1:649336/1‑247 (MQ=255)                                                                                                                                                                       gATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACaa                                                                       >  1:370329/1‑251 (MQ=255)                                                                                                                                                                          aCGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAg                                                                                            <  1:648857/227‑1 (MQ=255)                                                                                                                                                                           cGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTAccc                                                                                                   <  1:586564/219‑1 (MQ=255)                                                                                                                                                                           cGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCag                                                                   >  1:361988/1‑251 (MQ=255)                                                                                                                                                                           cGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCag                                                                   >  1:471317/1‑251 (MQ=255)                                                                                                                                                                             ttCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGaaataaat                                                                                                                                                                                                        >  1:160164/1‑116 (MQ=255)                                                                                                                                                                             ttCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCtt                                                                                                                                                                             >  1:75989/1‑143 (MQ=255)                                                                                                                                                                             ttCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATc                                                                                                                                                 <  1:17189/171‑1 (MQ=255)                                                                                                                                                                               cTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTa                                                                                                                                 <  1:614363/185‑1 (MQ=255)                                                                                                                                                                               cTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCagagag                                                               >  1:210039/1‑251 (MQ=255)                                                                                                                                                                                    aTAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGtt                                                                                                                                                      >  1:625784/1‑159 (MQ=255)                                                                                                                                                                                    aTAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGt                                                          >  1:103186/1‑251 (MQ=255)                                                                                                                                                                                         gaagaaACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAAtt                                                     >  1:683709/1‑251 (MQ=255)                                                                                                                                                                                         gaagaaACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAAtt                                                     >  1:348929/1‑251 (MQ=255)                                                                                                                                                                                                  aTCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTg                                                                                                                                                                                   >  1:364970/1‑116 (MQ=255)                                                                                                                                                                                                                     tAAATATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAgg                         <  1:523055/251‑1 (MQ=255)                                                                                                                                                                                                                       aaTATCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAGGAg                       <  1:781800/251‑1 (MQ=255)                                                                                                                                                                                                                        atatCAAGTTCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTACCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTAccc                                                                                                   >  1:307966/1‑174 (MQ=255)                                                                                                                                                                                                                                 tCAGCCGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAGGAGCGATAGATac             <  1:787091/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGGGTTGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAGGAGCGATAGATACACGTc        <  1:527444/251‑1 (MQ=255)                                                                                                                                                                                                                                           tGCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAGGAGCGATAGATACACGTCCACCt   <  1:399102/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCTTTCAGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAGGAGCGATAGATACACGTCCACCTa  >  1:234149/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     GTTATAACCATCAGACGTCTTTGAGACTGTAATTGGTCGTTTTAAACCAACAGACGCAATATTTTCTACAAGATCTGCGAAGATTTTTCGATTACGCGTTCTTGGATTGATAATCCGAATATCTTCTATCGGAATAGTTTGAATATCCATAATGAACTCCTAATAAGATACGTTCTACCATAATGAAGAAACGATCCAAGTTAGAAAATCGATAAATATCAAGTTCAGCCGGGTTGCTTTCCGCCAATCTCAATTTATTACTTTCTCTCCCCTTCATTGGAAATAAATAATAATCAATAATGTTTTCTGCATCTTCGTCAAGACGAACTGCAATAGTTAAATCAGGTTCAAGCCCGGTATCAAAACGAATCATCCAGCGTTTATTACCCCTTGTAGTTGGTCGGCAAGGGCTTACAACCAGAGAGGCGGTAAATTCTTCATTGATTTTTAGAAGATCATTAGGAGCGATAGATACACGTCCACCTA  >  1/289904‑290389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑