Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,307,149	G→A	intergenic (+147569/+113788)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,307,149	0	G	A	100.0%	66.5 / NA	27	intergenic (+147569/+113788)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/11);  total (16/11)

ACACCGTTCCTGAAATCACCAACCCCACCAAAGAAGCCAACCATGTTTTTGTGTGCATAAGATTTTCCTGTTTAAAAGATATACTGTTGATTAGATGAGCGTATCGTCTATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACGTATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGACAAGAATAAAAAATGCGAAAAGTTTTAAAA  >  1/1306927‑1307332                                                                                                                                                                                                                               |                                                                                                                                                                                        acacCGTTCCTGAAATCACCAACCCCACCAAAGAAGCCAACCATGTTTTTGTGTGCATAAGATTTTCCTGTTTAAAAGATATACTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAac                                                                                                                                                             <  1:147877/251‑1 (MQ=255)                                         ccATGTTTTTGTGTGCATAAGATTTTCCTGTTTAAAAGATATACTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACa                                                                                                                                                     >  1:764738/1‑219 (MQ=255)                                                                   ccTGTTTAAAAGATATACTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAAc                                                                                           <  1:664625/251‑1 (MQ=255)                                                                            aaGATATACTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAAcc                                                                                     >  1:768109/1‑248 (MQ=255)                                                                            aaGATATACTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAAt                                                                                  >  1:809627/1‑251 (MQ=255)                                                                            aaGATATACTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAAt                                                                                  >  1:730142/1‑251 (MQ=255)                                                                                  taCTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCACACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTTCATATTATTTTCATTTTATGGATGTAAAACACCGAGCAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAAc                                                                            <  1:832206/251‑1 (MQ=255)                                                                                    cTGTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAAcc                                                                                     <  1:312579/240‑1 (MQ=255)                                                                                      gTTGATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAAt                                                                                                                      >  1:900721/1‑205 (MQ=255)                                                                                         gATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAac                                                                                                                                                             <  1:707265/163‑1 (MQ=255)                                                                                         gATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAAt                                                                                                               <  1:139164/209‑1 (MQ=255)                                                                                         gATTAGATGAGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGgcg                                                                     >  1:641532/1‑251 (MQ=255)                                                                                                  aGCGTATCGTCGATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAggg                                                                                                                           >  1:715202/1‑188 (MQ=255)                                                                                                          gtcgaTGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAAc                                                                                                                                >  1:222845/5‑175 (MQ=255)                                                                                                                gAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAggg                                                                       <  1:521142/226‑1 (MQ=255)                                                                                                                 aGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAac                                                                                                                                                             >  1:544446/1‑139 (MQ=255)                                                                                                                  gTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAac                                                                                                                                                             >  1:393433/1‑138 (MQ=255)                                                                                                                  gTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTa                                            >  1:614234/1‑251 (MQ=255)                                                                                                                              tAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAg                                 <  1:512036/250‑1 (MQ=255)                                                                                                                               aaCAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGAc                               <  1:714315/251‑1 (MQ=255)                                                                                                                                aCAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGACa                              <  1:504807/251‑1 (MQ=255)                                                                                                                                                             cTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGAc                               >  1:463484/1‑221 (MQ=255)                                                                                                                                                             cTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGAc                               >  1:846559/1‑221 (MQ=255)                                                                                                                                                             cTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGACAAGAATaaaaa                    >  1:164242/1‑232 (MQ=255)                                                                                                                                                                              cATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAggg                                                                       >  1:704003/1‑164 (MQ=255)                                                                                                                                                                              cATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGACAAGAATAAAAAATGCGAAAAGTTTTaaaa  >  1:139795/1‑233 (MQ=255)                                                                                                                                                                                   aGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACATATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAAt                                                                                                               <  1:87784/119‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                        ACACCGTTCCTGAAATCACCAACCCCACCAAAGAAGCCAACCATGTTTTTGTGTGCATAAGATTTTCCTGTTTAAAAGATATACTGTTGATTAGATGAGCGTATCGTCTATGAGTTCGGTAGGTGTAACAAAAAAGTGCGGTTGATTTTCACCGCACTTAAAAATCATATAAACATCAAGTCAATATCACTTGAAAATGTAACTCGTTTTACTAAAATTTACGTATTATTTTCATTTTATGGATGTAAAACACCGAACAAAATGAGAAAAAGGAATAAACTAGGGAGAATGTTGAATCAAGCGGGAATAAGTGGAACAAAACCAATGAAAACAAGGGCGCAAGAAAAAACGGCGTCCTTTTTTATTGAAGAATAGACAAGAATAAAAAATGCGAAAAGTTTTAAAA  >  1/1306927‑1307332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑