Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,305,833	A→G	intergenic (+146253/+115104)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,305,833	0	A	G	100.0%	135.4 / NA	50	intergenic (+146253/+115104)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/22);  total (28/22)

TGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCGATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAATGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCACACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCAAGGCTGATC  >  1/1305611‑1306061                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     tGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTcc                                                                                                                                                                                                           >  1:732043/1‑250 (MQ=255) tGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTcc                                                                                                                                                                                                           >  1:87170/1‑250 (MQ=255)         cTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGt                                                                                                                                                                                                  >  1:111146/1‑251 (MQ=255)           aTCCACCCACTGAACCAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTTCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGc                                                                                                                                                                                                <  1:261573/251‑1 (MQ=255)                  cacTGAAACACCTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCCCCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGcacc                                                                                                                                                                                                                                  <  1:479642/210‑1 (MQ=255)                                aTCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACgg                                                                                                                                                                           >  1:886790/1‑251 (MQ=255)                                             tCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGatt                                                                                                                                                              >  1:35027/1‑251 (MQ=255)                                                   gCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCa                                                                                                                                                         >  1:856688/1‑250 (MQ=255)                                                          gCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGCATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTCCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGCTTTc                                                                                                                                                  >  1:395143/1‑250 (MQ=255)                                                               ccccATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGatt                                                                                                                                                              >  1:252296/1‑233 (MQ=255)                                                               ccccATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTAc                                                                                                                                             >  1:861637/1‑250 (MQ=255)                                                                      gCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTgtgt                                                                                                                                                                                           >  1:84188/1‑197 (MQ=255)                                                                         tttCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAatta                                                                                                                                  >  1:512607/1‑251 (MQ=255)                                                                           tCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGc                                                                                                                                                                   >  1:347831/1‑216 (MQ=255)                                                                           tCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAattatt                                                                                                                                >  1:432274/1‑251 (MQ=255)                                                                                   acggtGCTTCCCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGcc                                                                                                                          <  1:860908/245‑1 (MQ=255)                                                                                                gCCATACGCAATTTTATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGc                                                                                                                                                                                                >  1:417289/1‑166 (MQ=255)                                                                                                           ttttATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATg                                                                                                <  1:744244/251‑1 (MQ=255)                                                                                                            tttATAGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGc                                                                                               <  1:145930/251‑1 (MQ=255)                                                                                                                taGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGt                                                                                           >  1:95481/1‑251 (MQ=255)                                                                                                                 aGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTc                                                                                                                                                                                                     <  1:787782/144‑1 (MQ=255)                                                                                                                 aGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGc                                                                                                                                                                   <  1:147507/178‑1 (MQ=255)                                                                                                                 aGCTAACATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTg                                                                                          >  1:387728/1‑251 (MQ=255)                                                                                                                      aCATAGCACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGAt                                                                                     >  1:45715/1‑251 (MQ=255)                                                                                                                            cACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTccc                                                                                                                                                                                                          >  1:854424/1‑128 (MQ=255)                                                                                                                            cACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTg                                                                                                                                                                                         <  1:679117/145‑1 (MQ=255)                                                                                                                            cACCAATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACgg                                                                                                                                                      <  1:779200/180‑1 (MQ=255)                                                                                                                                           aaGTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGa                                                                >  1:680738/1‑251 (MQ=255)                                                                                                                                             gTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAGCTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAat                                                              >  1:720349/1‑251 (MQ=255)                                                                                                                                             gTATCACGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAat                                                              >  1:21785/1‑251 (MQ=255)                                                                                                                                                  aCGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAAc                                                         <  1:483800/251‑1 (MQ=255)                                                                                                                                                  aCGGTCGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAAc                                                         <  1:719169/251‑1 (MQ=255)                                                                                                                                                       cGGAGGTGCCGACCCGAGTGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTcc                                                    <  1:127297/251‑1 (MQ=255)                                                                                                                                                                         tGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAac                                   <  1:751843/250‑1 (MQ=255)                                                                                                                                                                           cgcCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCATATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCg                                                       <  1:454661/228‑1 (MQ=255)                                                                                                                                                                                     gcGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAAc                                                         >  1:370468/1‑216 (MQ=255)                                                                                                                                                                                        ttttACTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGAc                    <  1:759255/250‑1 (MQ=255)                                                                                                                                                                                             cTTTGATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCcaatc                                               <  1:60073/218‑1 (MQ=255)                                                                                                                                                                                                 gATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATg                                                                                                >  1:665882/1‑165 (MQ=255)                                                                                                                                                                                                 gATCCACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCaa          >  1:114959/1‑251 (MQ=255)                                                                                                                                                                                                    ccACATAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCAAGGc       <  1:506456/251‑1 (MQ=255)                                                                                                                                                                                                      acaTAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCAAgg        >  1:504345/1‑248 (MQ=255)                                                                                                                                                                                                      acaTAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCAAgg        >  1:43536/1‑248 (MQ=255)                                                                                                                                                                                                         tAATTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCAAGGCTGATc  <  1:588023/251‑1 (MQ=255)                                                                                                                                                                                                          aaTTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTAtttt                                                                                                                              <  1:800316/126‑1 (MQ=255)                                                                                                                                                                                                           aTTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACgg                                                                                                                                                                           >  1:261861/1‑80 (MQ=255)                                                                                                                                                                                                           aTTCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGt                                          <  1:814785/209‑1 (MQ=255)                                                                                                                                                                                                            ttCATGGCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCAAGGCTGATc  <  1:325119/248‑1 (MQ=255)                                                                                                                                                                                                                  gCGTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGc                        <  1:502284/220‑1 (MQ=255)                                                                                                                                                                                                                    gTTCATTAAGCGCACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCAc              >  1:494981/1‑228 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     TGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCGATAGCGTGCGAAGTATCACGGTCGGAGGTGCCGACCCGAATGCGCCAATGCTGCGTTTTACTTTGATCCACATAATTCATGGCGTTCATTAAGCACACCACATTTTTATCCGCCATCTTTCCCTTTTCCGTGCTGTGTTGCCAAGAAAAAACGGTGAAATGCTGATTATTCACGGTTTCCGTACCAAATAAATTATTTTCGCCCGAACTCAAATTCAGCGCATCAAATGCCGGTGGCGATTTCATTCGTTTTTCCGCGTGAATATAACCGTCCCAATCAATGTCGGTAACACCTTCTTTGCTGACGTTCACCCAAGGCTGATC  >  1/1305611‑1306061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑