Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,305,511	G→C	intergenic (+145931/+115426)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,305,511	0	G	C	100.0%	180.9 / NA	64	intergenic (+145931/+115426)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (34/30);  total (34/30)

GTTTACAGTTCTATGGCGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGT‑CCCCCTAAGTTATAGGGTGATTATTTTTCAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCGACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCGATAGCG  >  1/1305292‑1305744                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          gTTTACAGTTCTATGGCGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGct                                                                                                                                                                                                                                    <  1:128865/228‑1 (MQ=255)        gTTCTATGGCGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTACGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTAc                                                                                                                                                                                                       <  1:426891/250‑1 (MQ=255)          tCTATGGCGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACagt                                                                                                                                                                                                    <  1:113416/251‑1 (MQ=255)            tATGGCGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACagtag                                                                                                                                                                                                  <  1:339541/251‑1 (MQ=255)            tATGGCGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACagtag                                                                                                                                                                                                  <  1:176779/251‑1 (MQ=255)                 cGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTagtgagt                                                                                                                                                                                             <  1:884981/251‑1 (MQ=255)                   ggTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTagtgagtg                                                                                                                                                                                            >  1:571240/1‑250 (MQ=255)                   ggTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTagtgagtg                                                                                                                                                                                            >  1:23074/1‑250 (MQ=255)                   ggTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTagtga                                                                                                                                                                                               >  1:171454/1‑247 (MQ=255)                                tAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGt                                                                                                                                                                              <  1:273729/251‑1 (MQ=255)                                          atGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAAcacca                                                                                                                                                                     >  1:283220/1‑250 (MQ=255)                                            gtgAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTagtg                                                                                                                                                                                                >  1:264137/1‑221 (MQ=255)                                            gtgAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCg                                                                                                                                                                  >  1:782896/1‑251 (MQ=255)                                                        cgcTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCa                                                                                                                                                       <  1:380791/250‑1 (MQ=255)                                                                 gtTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTaaa                                                                                                                                             >  1:752418/1‑251 (MQ=255)                                                                  tttAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAAc                                                                                                                                            <  1:556398/251‑1 (MQ=255)                                                                  tttAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAAc                                                                                                                                            <  1:330502/251‑1 (MQ=255)                                                                  tttAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAAc                                                                                                                                            <  1:656798/251‑1 (MQ=255)                                                                      gCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTAtt                                                                                                                                        >  1:439410/1‑251 (MQ=255)                                                                                gTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATAc                                                                                                                               <  1:142350/250‑1 (MQ=255)                                                                                    tttATGAAAAGTGCCGTTTTTGCTTTTATATGTCCCCCCTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATcc                                                                                                                          <  1:770002/251‑1 (MQ=255)                                                                                                                 atgtccccccTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATa                                                                                             >  1:789671/6‑251 (MQ=255)                                                                                                                     ccccccTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATcc                                                                                         <  1:586145/250‑1 (MQ=255)                                                                                                                      cccccTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATcc                                                                                         >  1:683426/1‑250 (MQ=255)                                                                                                                       ccccTAAGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAaac                                                                                                              <  1:613032/228‑1 (MQ=255)                                                                                                                            aaGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACagtag                                                                                                                                                                                                  >  1:582456/1‑139 (MQ=255)                                                                                                                            aaGTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTTATCCAAATCATAATCCCCCGCAt                                                                                  >  1:863007/1‑251 (MQ=255)                                                                                                                              gTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAAc                                                                                                                                            >  1:786870/1‑191 (MQ=255)                                                                                                                              gTTATAGGGTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATg                                                                                 >  1:858190/1‑250 (MQ=255)                                                                                                                                    gggTGATTATTTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATcc                                                                                         >  1:615348/1‑236 (MQ=255)                                                                                                                                            attTTTTAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACT‑AAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTCCCATTCCCGTAAACTATTTGCTAATAc                                                                                                                               <  1:435402/189‑1 (MQ=255)                                                                                                                                                     ccaccCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATa                                                         >  1:807806/1‑251 (MQ=255)                                                                                                                                                       accCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATG‑TTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAAt                                                       >  1:633462/1‑250 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACagtag                                                                                                                                                                                                  <  1:236218/111‑1 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGt                                                                                                                                                                              <  1:266063/131‑1 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATAc                                                                                                                               <  1:762143/178‑1 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATAc                                                                                                                               <  1:785829/178‑1 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATAc                                                                                                                               >  1:355034/1‑178 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGccc                                                                       <  1:2849/234‑1 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATgg                                                                  <  1:489322/239‑1 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAAt                                                       >  1:748364/1‑250 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAAt                                                       >  1:117302/1‑250 (MQ=255)                                                                                                                                                        cccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCC‑CTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATc                                                      >  1:312321/1‑250 (MQ=255)                                                                                                                                                         ccTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATc                                                      >  1:95064/1‑250 (MQ=255)                                                                                                                                                             aGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATAc                                                                                                                               <  1:137463/173‑1 (MQ=255)                                                                                                                                                              ggTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCAc                                                   >  1:513908/1‑248 (MQ=255)                                                                                                                                                               gTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACagt                                                                                                                                                                                                    >  1:75082/1‑102 (MQ=255)                                                                                                                                                               gTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGc                                                                 >  1:460868/1‑233 (MQ=255)                                                                                                                                                               gTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTg                                                >  1:593242/1‑250 (MQ=255)                                                                                                                                                               gTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTg                                                >  1:706440/1‑250 (MQ=255)                                                                                                                                                                        tGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAac                                                                                                                                                                         <  1:527347/120‑1 (MQ=255)                                                                                                                                                                         gCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCAcc                                                  <  1:720030/238‑1 (MQ=255)                                                                                                                                                                                    tCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATg                                                                                                                                                                                      >  1:706712/1‑95 (MQ=255)                                                                                                                                                                                       cAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTtata                       >  1:335245/1‑251 (MQ=255)                                                                                                                                                                                          tGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGc                     <  1:229867/250‑1 (MQ=255)                                                                                                                                                                                                  gCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCa                                                                                                                                                       >  1:576519/1‑112 (MQ=255)                                                                                                                                                                                                  gCCCAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTtat                        >  1:584008/1‑239 (MQ=255)                                                                                                                                                                                                   cccAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGc                                                                 <  1:17968/197‑1 (MQ=255)                                                                                                                                                                                                    ccAATGTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCAc          <  1:697789/251‑1 (MQ=255)                                                                                                                                                                                                         gTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTAc                                                                                                                                                                                                       >  1:93793/1‑57 (MQ=255)                                                                                                                                                                                                         gTTTTGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATa     >  1:356625/1‑251 (MQ=255)                                                                                                                                                                                                          ttttGATAGCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCCCCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAg    <  1:477184/251‑1 (MQ=255)                                                                                                                                                                                                            ttGATAGCCAGAAGCCCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAACCACCCCCGCACTTTTACCATTCCCGTAACCTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAACCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTCCCCGCCATACGCAATTTTATAGCTAACATAGCACCAATAGCg  <  1:346382/251‑1 (MQ=255)                                                                                                                                                                                                                  gCCAGAAGCTCCACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAAc                                                                                                                                            >  1:691522/1‑107 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          GTTTACAGTTCTATGGCGGGTTATTATTCCGTAAAAAAATAATGTGAAACAACCGCGCTTATGTGTTTAGCATACAACGGTGCTTTATGAAAAGTGCCGTTTTTGCTTTTATATGT‑CCCCCTAAGTTATAGGGTGATTATTTTTCAACCACCCTTAGGTATTACATTGCTATTTCATGTCCCAGTGGAACAGGCCCAATGTTTTGATAGCCAGAAGCTCGACAAGCTCTGACTCAAGCTCAACGATCGTCCTCTACAGTAGTGAGTGCCGATGCTTTATGTAAAACACCACCGCACTTTTACCATTCCCGTAAACTATTTGCTAATACTATCCACCCACTGAAACAACTCATCCAAATCATAATCCCCCGCATGCGGCACGCCCCATGGCGTTTCATAATCCACCTGCTTACCCGCCATACGCAATTTTATAGCTAACATAGCACCGATAGCG  >  1/1305292‑1305744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑