Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,301,714	T→C	intergenic (+142134/+119223)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,301,714	0	T	C	100.0%	191.2 / NA	67	intergenic (+142134/+119223)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/28);  total (39/28)

ATTTTATCCGACAATAAAACCGTTTATCACGCCAAAATTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGTTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAATATAAT  >  1/1301471‑1301959                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      aTTTTATCCGACAATAAAACCGTTTATCACGCCAAAATTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTc                                                                                                                                                                                                                                                 >  1:584820/1‑250 (MQ=255)            cAATAAAACCGTTTCTCACGCCAAAATTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAg                                                                                                                                                                                                                                     >  1:421608/1‑251 (MQ=255)                     cGTTTATCACGCCAAAATTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAagc                                                                                                                                                                                                                            <  1:241535/251‑1 (MQ=255)                                  aaaaTTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAagc                                                                                                                                                                                                                            <  1:523141/238‑1 (MQ=255)                                  aaaaTTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAata                                                                                                                                                                                                               <  1:827350/251‑1 (MQ=255)                                  aaaaTTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAata                                                                                                                                                                                                               <  1:459481/251‑1 (MQ=255)                                         aTTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTc                                                                                                                                                                                                        <  1:441684/251‑1 (MQ=255)                                                   cccGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTaa                                                                                                                                                                                              >  1:745730/1‑251 (MQ=255)                                                             accaccATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATac                                                                                                                                                                                    <  1:735810/251‑1 (MQ=255)                                                                             aTTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGgaa                                                                                                                                                                    >  1:396446/1‑251 (MQ=255)                                                                                  aGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTAc                                                                                                                                                                                                 >  1:726523/1‑217 (MQ=255)                                                                                  aGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAacac                                                                                                                                                                               >  1:837558/1‑235 (MQ=255)                                                                                            gTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTg                                                                                                                                                     >  1:729879/1‑251 (MQ=255)                                                                                            gTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTAGATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATac                                                                                                                                                                                    <  1:455326/220‑1 (MQ=255)                                                                                                       ggTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGAttt                                                                                                                                          >  1:307626/1‑251 (MQ=255)                                                                                                                          gTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATc                                                                                                                        >  1:105106/1‑250 (MQ=255)                                                                                                                                      gcgcAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGaa                                                                                                           >  1:805854/1‑251 (MQ=255)                                                                                                                                         cAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGc                                                                                                                                                                                                                                       <  1:313105/124‑1 (MQ=255)                                                                                                                                         cAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAg                                                                                                                                                                                                          >  1:85672/1‑153 (MQ=255)                                                                                                                                         cAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAgg                                                                                                         >  1:532411/1‑250 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAagc                                                                                                                                                                                                                            <  1:639300/133‑1 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAAc                                                                                                                                                                                            >  1:86654/1‑165 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCACTACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGa                                                                                                      >  1:441752/1‑251 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAg                                                                                                                                                                            >  1:565298/1‑181 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGa                                                                                                      >  1:872665/1‑251 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGa                                                                                                      >  1:63963/1‑251 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGa                                                                                                      >  1:14396/1‑251 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGa                                                                                                      >  1:468086/1‑251 (MQ=255)                                                                                                                                           gTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGa                                                                                                      >  1:200260/1‑251 (MQ=255)                                                                                                                                             atatTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAg                                                                                                                                                                            >  1:375179/1‑179 (MQ=255)                                                                                                                                                  attaCGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACgg                                                                                                             >  1:286506/1‑237 (MQ=255)                                                                                                                                                   ttaCGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAagtag                                                                                              <  1:35574/251‑1 (MQ=255)                                                                                                                                                      cGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAatat                                                                                                                                                                                                              <  1:858214/136‑1 (MQ=255)                                                                                                                                                      cGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGa                                                                                           >  1:573811/1‑251 (MQ=255)                                                                                                                                                             aTTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTcaca                                                                                    <  1:97970/251‑1 (MQ=255)                                                                                                                                                                          aTCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGt                                                                       <  1:199151/251‑1 (MQ=255)                                                                                                                                                                            cccGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGaa                                                                                                                                                                          >  1:18546/1‑150 (MQ=255)                                                                                                                                                                                     gTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGGGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTg                                                                                            <  1:430403/219‑1 (MQ=255)                                                                                                                                                                                          gCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTaa                                                                                                                                                                                              <  1:572372/116‑1 (MQ=255)                                                                                                                                                                                          gCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGAt                                                        >  1:163530/1‑250 (MQ=255)                                                                                                                                                                                          gCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGAAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGAt                                                        >  1:527193/1‑250 (MQ=255)                                                                                                                                                                                            gTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAatat                                                                                                                                                                                                              >  1:777426/1‑98 (MQ=255)                                                                                                                                                                                            gTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATc                                                       >  1:564767/1‑249 (MQ=255)                                                                                                                                                                                              aTTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAag                                                                                                 <  1:53913/205‑1 (MQ=255)                                                                                                                                                                                              aTTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGa                                                   >  1:199968/1‑251 (MQ=255)                                                                                                                                                                                                tGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAagc                                                                                                                                                                                                                            >  1:178577/1‑80 (MQ=255)                                                                                                                                                                                                tGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATg                                                 <  1:717689/251‑1 (MQ=255)                                                                                                                                                                                                tGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATg                                                 <  1:725188/251‑1 (MQ=255)                                                                                                                                                                                                 ggTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATg                                                                                                                                                                                                             <  1:658872/94‑1 (MQ=255)                                                                                                                                                                                                 ggTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAagt                                                                                                >  1:23044/1‑203 (MQ=255)                                                                                                                                                                                                 ggTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATg                                                 >  1:276925/1‑250 (MQ=255)                                                                                                                                                                                                  gTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGc                                                <  1:399120/250‑1 (MQ=255)                                                                                                                                                                                                       aCCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATacaac                                                                                                                                                                                 <  1:64562/116‑1 (MQ=255)                                                                                                                                                                                                           cGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTAtc                                      <  1:359181/251‑1 (MQ=255)                                                                                                                                                                                                             gAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATc                                                                                                                        <  1:99564/167‑1 (MQ=255)                                                                                                                                                                                                             gAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTAtctt                                    >  1:94994/1‑251 (MQ=255)                                                                                                                                                                                                                     cATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAg                              <  1:706606/249‑1 (MQ=255)                                                                                                                                                                                                                     cATTAAAATTGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTg                            >  1:337767/1‑251 (MQ=255)                                                                                                                                                                                                                             ttGCCGATGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGaa                                                                                                                                                                          <  1:124529/101‑1 (MQ=255)                                                                                                                                                                                                                                   aTGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTcgac              <  1:582959/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTGTGCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTcgac              <  1:406482/251‑1 (MQ=255)                                                                                                                                                                                                                                     gtgCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAGCGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAAcc                                                                 <  1:571494/198‑1 (MQ=255)                                                                                                                                                                                                                                     gtgCAAAATGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTcgacga            >  1:745466/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTGTGTTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAatat     >  1:211856/1‑251 (MQ=255)                                                                                                                                                                                                                                              gtgtTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAATATaa   >  1:643224/1‑251 (MQ=255)                                                                                                                                                                                                                                              gtgtTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAATATaa   >  1:531542/1‑251 (MQ=255)                                                                                                                                                                                                                                              gtgtTGCTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGCGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAATATaa   >  1:320212/1‑251 (MQ=255)                                                                                                                                                                                                                                               tgttgcTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAATATAAt  <  1:742390/251‑1 (MQ=255)                                                                                                                                                                                                                                               tgttgcTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAATATAAt  <  1:630520/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ATTTTATCCGACAATAAAACCGTTTATCACGCCAAAATTGATTCCGTAGACCCGGCAACAACCACCATCAGCGACAATTCAAGCTCAAACAGTTCAAGCTCAGGTTCCAGTTCCTCCTCCGGTTCAAGCAGCAGCGCAGTATATTATTACGCCAACATTATCGTTGAAAATCCCGAACATGTTTTGCGTATTGGTATGACCACGGAAAATAACATTAAAATTGCCGATGTGCAAAATGTGTTGTTTATTCCGAATTTGGCAGTGCAAAAGCAGCAAGACAAATATGTAGTCAATGTACTAAACGGCAATACAACACAAGAACGGGAAATCGAAATCGGCGTGCAAAATGATTTCCAAACGGAAGTCAAATCCGGTTTAACGGAAGGCGAAAAAGTAGTGATTTCACAAGTTGCTGCCGGTGAAACCTTCGGCGATCCTGATGCCCCGATTATCTTCTAAAGTGCGGTGGGTTTCGACGATGAATATAAT  >  1/1301471‑1301959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑