| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,300,823 | G→A | intergenic (+141243/+120114) | unknown → / ← unknown | tRNA‑Val‑GAC/tRNA‑Met‑CAT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,300,823 | 0 | G | A | 85.7% | 72.2 / ‑2.1 | 35 | intergenic (+141243/+120114) | unknown/unknown | tRNA‑Val‑GAC/tRNA‑Met‑CAT |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/20); minor base C (5/0); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.25e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CATAAAAAGCGAGGAATGCGGCTTATTGTTGAAATAAATGTGCAAACTACCCTTATTTTTAATTGAGTATCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCGGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGAAAAAAGGCGATTTAATTGCCGAGATTGATTCCACCACCCAAATTAATA > 1/1300578‑1301030 | cATAAAAAGCGAGGAATGCGGCTTATTGTTGAAATAAATGTGCAAACTACCCTTATTTTTAATTGAGTATCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTTCGCGGTTCCAgcg‑c > 1:782645/1‑250 (MQ=255) aaaGCGAGGAATGCGGCTTATTGTTGAAATAAATGTGCAAACTACCCTTATTTTTAATTGAGTATCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTTTTTTTTTTTTTGCGATTCCAGCA‑CACaaaa > 1:218815/1‑250 (MQ=255) aaGCGAGGAATGCGGCTTATTGTTGAAATAAATGTGCAAACTACCCTTATTTTTAATTGAGTATCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAAcc < 1:326357/251‑1 (MQ=255) aaTGCGGCTTATTGTTGAAATAAATGTGCAAACTACCTTTATTTTTAATTGAGTATCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCacttact < 1:515189/250‑1 (MQ=255) ttgttgAAATAAATGTGCAAACTACCCTTATTTTTAATTGAGTATCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTaa < 1:710502/242‑1 (MQ=255) aTCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTACGCGATTTCAGCG‑CAAAAAACACCTACTTaaag > 1:685730/1‑199 (MQ=255) aTCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑cac < 1:872817/184‑1 (MQ=255) tCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTACGCGGTTCCCGCG‑CACAAAACACTTACTTTAC‑GGAagag > 1:56299/1‑206 (MQ=255) gaATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGCTTTTTTTTTTTAAGCGCTTCCAGCG‑CACAAAACACCTACTTAAC‑CGGAGAGGGGAAAACCGGCAATATTTAAAAGAAAGAGGTTGCTTCCGGGTCCCATCGaaga > 1:224867/1‑249 (MQ=255) cAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATAc < 1:594922/250‑1 (MQ=255) tttCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTACGCGATTCCCGCG‑CACAAACCACCTACTTTAA‑GGAAGAGGGCAAAAGCGGCAAAATTTAAAAAAAAAAGGGTGCTACC‑GGGTCCATCGAAAAAATCAATACTGTTTATTTGGGGGGGCAgggggg > 1:727897/1‑247 (MQ=255) ttCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCacttac < 1:703714/152‑1 (MQ=255) ttgttgTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTg < 1:40478/173‑1 (MQ=255) ttgttgTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGCTTTTTTTTTGTTCACAATTCCACCG‑CACCAAACACTTACTTAAC‑GGAAGAGGGCAAACGCGGCAATATTGAAAAAAAAGCGGTTGCTACC‑CGTTCCATCGAAAATATCAATACTGTTGTTGTTGGGGCGCCGGGGTCA‑GGGAAAGtaa > 1:406803/1‑249 (MQ=255) gttgtATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTTTTTTTTTTTTCGCGATTCCCGCGCCCCAAACC‑CTTCCTTTAC‑GGGAGAGGGCAAACACGGCCATATTTTAAAAAAAGTGGGTGCTTCC‑GGGTTCATCTAAAAAATCAATACTGTTTTTGTGGGGGCGCAGGGGTCA‑GGGGAAGGCAc > 1:437108/1‑250 (MQ=255) gttgtATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTTCGCGATTTCAGCG‑CACAAACCACTTACTTAAc > 1:855885/1‑145 (MQ=255) gttgtATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTTCGCGATTTCAGCG‑CACAAACCACCTACTTAAC‑GGAAGAGGGCAAACACGGCCATATTTAAAAAAAAGTGGTTGCTAcccggt > 1:50827/1‑191 (MQ=255) gttgtATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTggg < 1:754424/226‑1 (MQ=255) gttgtATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGATTTTTTTTTTTTCGCGCTTTCAGCG‑CACCAACCCCCTACTTAAA‑GGAAGAGGGCAAACGCGGGAATATTTAAAAGAAAGAGGGTTCTACC‑CGGTCCATCCAAAGTATCAATACTGTTGATGTGGGGGGGCAGGGGTCA‑GGGGAAgggaa > 1:403679/1‑246 (MQ=255) gtgtGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTACGCGATTCCAGCG‑CACAAACCACCTACTTAAC‑GGAAGAGGGCAAAAGCGGCAATATTTAAAAAAAAGTGGGTGCTACC‑GGGTCCATCGAAAATATCAAAAATGTTGATGTGGGCGCGCAGGGGTCA‑ggg > 1:210317/1‑234 (MQ=255) gtgtGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGtt < 1:878531/187‑1 (MQ=255) aTTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAAc < 1:528935/251‑1 (MQ=255) cTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTACGCGCTTTCACCC‑CACAAACCCCCTACTTAACAGGAA‑AGGGCAAAAACGGCAAAATTTAAAAAAAAGAGGTTGCTACC‑CGGTCCATCCAAAAAATCAAAAATTTTTATTTGGGGGCGCAGGGGGCA‑GGGAAAGAAACCAAACATTATGTTAAAATGGGgcaacaag > 1:15885/1‑249 (MQ=255) agagaAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTCGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGaaa < 1:785732/250‑1 (MQ=255) aaaaaGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGAAAAAAgg < 1:82538/251‑1 (MQ=255) aagaaAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGAAAAAAGGCGATTTAAt < 1:702675/251‑1 (MQ=255) ccTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTACGCGATTCCCGCG‑CACAAACCACCTACTTAAC‑GGAAGAGGGCAAACGCGGCAATATTTAAAAAAAAGTGGGTGCTACC‑GGTTCCATCGAAAGGATCAATACTGTTGATGTGGGGGCGCCGGGGGCA‑GGGAAAGTAACCCAAATTTTTGTCAAAATGGGGCAAAAGGGGAAAAAAAGCGATTTAATTTCCgagag > 1:535260/1‑250 (MQ=255) ctctTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGAtgtg < 1:18176/167‑1 (MQ=255) tattattaCTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGAAAAAAGGCGATTTAATTGCCg < 1:256640/242‑1 (MQ=255) attaCTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAg < 1:272191/140‑1 (MQ=255) cTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTTTACGCGGTTTCCGCG‑CCCAAACCACCTACTTAAA‑GGGAGAGGGCAAAAGCGGCCAAATTTAAAAAAAAGGGGGTGCTACC‑GGGTCCCTCCAAAAAAATAAAAATGTTGATGTGGGGGCGCCGGGGGCAGGGGAAAG‑CACCAAACATTATGTTAAAATGGGGCAACAAGGGAAAAAAAGGGAATTAATTGCCGAGGATGATTCcaccac > 1:175128/1‑250 (MQ=255) gCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGaaa < 1:332401/103‑1 (MQ=255) ttGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATAGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGAAAAAAGTCGATTTAATTGCCGAGATTGATTCCACCACCCAAAtt < 1:887223/251‑1 (MQ=255) ctgctgTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGAAAAAAGGCGATTTAATTGCCGAGATTGATTCCACCACCCAAATTAATa < 1:735312/249‑1 (MQ=255) gATTACCGGCGGTTATTTTTTTGTACGCGATTCCAGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGAtg < 1:832924/134‑1 (MQ=255) | CATAAAAAGCGAGGAATGCGGCTTATTGTTGAAATAAATGTGCAAACTACCCTTATTTTTAATTGAGTATCATTGGGAGAATAATGGCAACACGCTAAAAATTTTAATAATTTCTTAAGTTTTGTTGTATAGTGTGGCGATTCATTGTGGACTTATGAGAGAAAAAGATGAAGAAAAAACCTCTTATTATTACTGCTATTGCAGCTGCTGTGATTACCGGCGGTTATTTTTTTGTACGCGATTCCGGCG‑CACAAACCACTTACTTAAC‑GGAAGAGGTCAAACGCGGCAATATTGAAAAGAACGTGGTTGCTACC‑GGTTCCATCGAAAGTATCAATACTGTTGATGTGGGCGCGCAGGTGTCA‑GGGAAAGTCACCAAACTTTATGTCAAACTGGGACAACAGGTGAAAAAAGGCGATTTAATTGCCGAGATTGATTCCACCACCCAAATTAATA > 1/1300578‑1301030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |