Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,299,549	A→G	intergenic (+139969/+121388)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,299,549	0	A	G	100.0%	196.4 / NA	68	intergenic (+139969/+121388)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/35);  total (33/35)

ATTGTTTATTCGCTTCTTCGCGGAAATCGGCACAATCAATATAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTAGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAACACCCAATCATACAAACGA  >  1/1299302‑1299789                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 atTGTTTATTCGCTTCTTCGCGGAAATCGGCACAATCAATATAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTc                                                                                                                                                                                                                                               <  1:198009/251‑1 (MQ=255)           cGCTTCTTCGCGGAAATCGGCACAATCAATATAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATac                                                                                                                                                                                                                                     <  1:266249/251‑1 (MQ=255)             cttcttCGCGGAAATCGGCACAATCAATATAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATacga                                                                                                                                                                                                                                   <  1:113271/251‑1 (MQ=255)                               cacaATCAATATAGATTTCTTTTGTGAGCGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATGGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATg                                                                                                                                                                                                                 <  1:274140/251‑1 (MQ=255)                                  aatcaatATAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGa                                                                                                                                                                                                               >  1:604099/1‑250 (MQ=255)                                       atatAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCgg                                                                                                                                                                                                         >  1:107336/1‑251 (MQ=255)                                       atatAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCgg                                                                                                                                                                                                         >  1:60470/1‑251 (MQ=255)                                            gATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATacga                                                                                                                                                                                                                                   <  1:818864/220‑1 (MQ=255)                                               ttCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAAc                                                                                                                                                                                                 <  1:399220/251‑1 (MQ=255)                                               ttCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAAc                                                                                                                                                                                                 <  1:119966/251‑1 (MQ=255)                                               ttCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAAc                                                                                                                                                                                                 <  1:32184/251‑1 (MQ=255)                                                tCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAAcc                                                                                                                                                                                                <  1:648791/251‑1 (MQ=255)                                                             gTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAAc                                                                                                                                                                                                 <  1:355070/237‑1 (MQ=255)                                                             gTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAg                                                                                                                                                                                   >  1:586070/1‑251 (MQ=255)                                                               aaTTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGcc                                                                                                                                                                                 <  1:879386/251‑1 (MQ=255)                                                                     cGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGaaa                                                                                                                                                                            <  1:256138/250‑1 (MQ=255)                                                                     cGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGaaa                                                                                                                                                                            <  1:281075/250‑1 (MQ=255)                                                                         ctcCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATac                                                                                                                                                                                                                                     <  1:463075/189‑1 (MQ=255)                                                                         ctcCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGtat                                                                                                                                                                                                    <  1:635077/222‑1 (MQ=255)                                                                               gTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGaa                                                                                                                                                                                                                   >  1:128633/1‑201 (MQ=255)                                                                               gTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCa                                                                                                                                                                                               >  1:294597/1‑221 (MQ=255)                                                                               gTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGaaa                                                                                                                                                                            >  1:386242/1‑240 (MQ=255)                                                                               gTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATa                                                                                                                                                                 >  1:261698/1‑251 (MQ=255)                                                                                  cTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATAcgc                                                                                                                                                              <  1:253985/251‑1 (MQ=255)                                                                                  cTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATAcgc                                                                                                                                                              <  1:568661/251‑1 (MQ=255)                                                                                  cTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATAcgc                                                                                                                                                              <  1:709166/251‑1 (MQ=255)                                                                                                tGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGcc                                                                                                                                                <  1:21285/251‑1 (MQ=255)                                                                                                  aTTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGt                                                                                                                                              >  1:760354/1‑251 (MQ=255)                                                                                                            gTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCg                                                                                                                                               <  1:490624/240‑1 (MQ=255)                                                                                                            gTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGAttt                                                                                                                                     >  1:57077/1‑250 (MQ=255)                                                                                                                 tatTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAAc                                                                                                                                                                                                 <  1:885661/185‑1 (MQ=255)                                                                                                                              tCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTa                                                                                                                   <  1:873810/250‑1 (MQ=255)                                                                                                                                 aaGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAagag                                                                                                               <  1:338209/251‑1 (MQ=255)                                                                                                                                   gTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCa                                                                                                                                                                                               <  1:852517/169‑1 (MQ=255)                                                                                                                                          aTCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGttt                                                                                                      <  1:637893/251‑1 (MQ=255)                                                                                                                                              ccacTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAagag                                                                                                               >  1:177164/1‑238 (MQ=255)                                                                                                                                               cccTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAgg                                                                                                 <  1:194239/249‑1 (MQ=255)                                                                                                                                               cacTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAgg                                                                                                 <  1:563726/251‑1 (MQ=255)                                                                                                                                                 cTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGtt                                                                                               <  1:827597/251‑1 (MQ=255)                                                                                                                                                     cACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAgg                                                                                           <  1:416066/251‑1 (MQ=255)                                                                                                                                                              gatcACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTa                                                                                  >  1:539606/1‑251 (MQ=255)                                                                                                                                                               atcACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTaa                                                                                 >  1:76958/1‑251 (MQ=255)                                                                                                                                                                   ccGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAAc                                                                                                                                                                                                 <  1:18771/135‑1 (MQ=255)                                                                                                                                                                     gCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAagag                                                                                                               >  1:806140/1‑215 (MQ=255)                                                                                                                                                                                  gtgCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCa                                                              >  1:71975/1‑251 (MQ=255)                                                                                                                                                                                      aTTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTtcatca                                                                                                                                >  1:89681/1‑181 (MQ=255)                                                                                                                                                                                                 aaTCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGAt                                                                                                                                                          >  1:80709/1‑144 (MQ=255)                                                                                                                                                                                                           aaTGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCaa                                     >  1:291465/1‑251 (MQ=255)                                                                                                                                                                                                            aTGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAgg                                                                                           <  1:199939/196‑1 (MQ=255)                                                                                                                                                                                                                 ggCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGa                               >  1:331804/1‑251 (MQ=255)                                                                                                                                                                                                                   cTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGc                                                                                        <  1:879293/192‑1 (MQ=255)                                                                                                                                                                                                                      cTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGt                                                                                          >  1:5838/1‑187 (MQ=255)                                                                                                                                                                                                                      cTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGt                                                                                          <  1:773399/187‑1 (MQ=255)                                                                                                                                                                                                                      cTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATAttt                          >  1:785021/1‑251 (MQ=255)                                                                                                                                                                                                                      cTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATAttt                          >  1:901104/1‑251 (MQ=255)                                                                                                                                                                                                                      cTAATGCACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATAttt                          >  1:651544/1‑251 (MQ=255)                                                                                                                                                                                                                            cACTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCaa                     >  1:109444/1‑250 (MQ=255)                                                                                                                                                                                                                              cTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATAtt                                                          >  1:326076/1‑211 (MQ=255)                                                                                                                                                                                                                              cTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAaca                   >  1:510151/1‑250 (MQ=255)                                                                                                                                                                                                                              cTGTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAaca                   >  1:266093/1‑250 (MQ=255)                                                                                                                                                                                                                                gTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTtcatca                                                                                                                                <  1:167465/139‑1 (MQ=255)                                                                                                                                                                                                                                gTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCAcc                                                                                                                          >  1:640208/1‑145 (MQ=255)                                                                                                                                                                                                                                gTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAACAcc                 >  1:253413/1‑250 (MQ=255)                                                                                                                                                                                                                                gTATTCCACCACGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTCAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAACAcc                 >  1:710755/1‑250 (MQ=255)                                                                                                                                                                                                                                          acGTTATCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAACACCCAATCATACaa      >  1:142141/1‑251 (MQ=255)                                                                                                                                                                                                                                               aTCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAgg                                                                                           >  1:178032/1‑161 (MQ=255)                                                                                                                                                                                                                                               aTCTTGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAACACCCAATCATACAAacga  >  1:134762/1‑250 (MQ=255)                                                                                                                                                                                                                                                   tGTTTGGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTagag                                                                                                  <  1:662271/150‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ATTGTTTATTCGCTTCTTCGCGGAAATCGGCACAATCAATATAGATTTCTTTTGTGAACGGTAATTGGCGTTCTCCTAGTTCTGGACGATTCGGGTGATTTGGCGCAGTCAATATTTCTTCACCGTCAAAGTTTTCAATCACCACTTTCACCGGATCGATCACCGCCATGGCGCGTGGTGCATTTTGGTTTAAATCATCACGAATGCAGGCTTCTAATGCACTGTATTCCACCACGTTATCTTGTTTAGTCACGCCAATACGACGGCAAAATTCACGAATGGACGCCGGTGTATAACCACGACGGCGCAAGCCTGAAATGGTTGGCATACGCGGATCGTTCCAGCCGTCCACGATTTCATCATTCACCAATTTTAAGAGTTTGCGTTTAGAGGTTAAGGTGCCTTCTAAATTTAAACGGGAAAATTCATATTGGTGCGGCAGCGGGCGTTCAATGCTGATATTTTCCAACACCCAATCATACAAACGA  >  1/1299302‑1299789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑