Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,298,651	T→C	intergenic (+139071/+122286)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,298,651	0	T	C	100.0%	218.7 / NA	76	intergenic (+139071/+122286)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/40);  total (36/40)

AATATCGGCTTTTTTCACGCTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTTCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCAAACTCACTGTCAGGTTGAATACCA  >  1/1298410‑1298893                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   aaTATCGGCTTTTTTCACGCTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTg                                                                                                                                                                                                                                            >  1:821744/1‑250 (MQ=255)  atatCGGCTTTTTTCACGCTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGcc                                                                                                                                                                                                                                          <  1:482720/251‑1 (MQ=255)           tttttCACGCTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTc                                                                                                                                                                                                                                 <  1:798338/251‑1 (MQ=255)                    cTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAatat                                                                                                                                                                                                                            <  1:733786/247‑1 (MQ=255)                    cTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATAttt                                                                                                                                                                                                                        >  1:441671/1‑251 (MQ=255)                    cTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGGTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATAttt                                                                                                                                                                                                                        >  1:223874/1‑251 (MQ=255)                      aTCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAat                                                                                                                                                                                                                              >  1:131110/1‑243 (MQ=255)                      aTCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTa                                                                                                                                                                                                                       >  1:693057/1‑250 (MQ=255)                      aTCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTa                                                                                                                                                                                                                       >  1:791086/1‑250 (MQ=255)                          ggATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAATCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGAt                                                                                                                                                                                                                   <  1:432558/250‑1 (MQ=255)                          ggATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGAt                                                                                                                                                                                                                   <  1:851917/250‑1 (MQ=255)                                   cTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACAttt                                                                                                                                                                                                         >  1:395643/1‑251 (MQ=255)                                     gATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCatat                                                                                                                                                                                                                                               >  1:605240/1‑211 (MQ=255)                                        aaGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCcac                                                                                                                                                                                                    <  1:857290/251‑1 (MQ=255)                                           cGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCacac                                                                                                                                                                                                  <  1:843732/250‑1 (MQ=255)                                           cGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCacac                                                                                                                                                                                                  <  1:348893/250‑1 (MQ=255)                                           cGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACAcc                                                                                                                                                                                                 <  1:391765/251‑1 (MQ=255)                                           cGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACAcc                                                                                                                                                                                                 <  1:607402/251‑1 (MQ=255)                                                  cATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTcaca                                                                                                                                                                                          >  1:110924/1‑251 (MQ=255)                                                   aTGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGGCAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACAt                                                                                                                                                                                         <  1:543085/251‑1 (MQ=255)                                                             cGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTcaca                                                                                                                                                                                          >  1:312036/1‑240 (MQ=255)                                                              ggTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCaa                                                                                                                                                                                                              >  1:421247/1‑219 (MQ=255)                                                               gTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTcaca                                                                                                                                                                                          <  1:334406/238‑1 (MQ=255)                                                                      ttCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCAt                                                                                                                                                                      <  1:832763/251‑1 (MQ=255)                                                                          tGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTc                                                                                                                                                                                                                                 <  1:215538/188‑1 (MQ=255)                                                                          tGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTa                                                                                                                                                                                                                       <  1:77399/198‑1 (MQ=255)                                                                          tGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACAt                                                                                                                                                                                         >  1:369777/1‑228 (MQ=255)                                                                          tGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGtt                                                                                                                                                                  >  1:233395/1‑251 (MQ=255)                                                                           gTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCAc                                                                                                                                                                             <  1:544483/239‑1 (MQ=255)                                                                            taataaACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATAtt                                                                                                                                                                                                                                              <  1:786346/173‑1 (MQ=255)                                                                            taataaACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTc                                                                                                                                                                 <  1:245249/250‑1 (MQ=255)                                                                            taataaACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTc                                                                                                                                                                 <  1:497674/250‑1 (MQ=255)                                                                                aaaCGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTc                                                                                                                                                            <  1:43053/251‑1 (MQ=255)                                                                                aaaCGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTc                                                                                                                                                            <  1:641503/251‑1 (MQ=255)                                                                                aaaCGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTc                                                                                                                                                            <  1:50895/251‑1 (MQ=255)                                                                                  aCGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAatat                                                                                                                                                                                                                            >  1:207824/1‑185 (MQ=255)                                                                                          cacaGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGTCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAgg                                                                                                                                                    <  1:449622/249‑1 (MQ=255)                                                                                              ggTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTtgt                                                                                                                                              >  1:626470/1‑251 (MQ=255)                                                                                               gTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGttt                                                                                                                                                 <  1:671507/247‑1 (MQ=255)                                                                                                tGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTgtgt                                                                                                                                            <  1:452190/251‑1 (MQ=255)                                                                                                  gCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCCGCATCGTTCATGTCAAGTAAGGTTTTGTGTAg                                                                                                                                          >  1:484734/1‑251 (MQ=255)                                                                                                    gggTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACg                                                                                                                                                                                                                     <  1:441204/174‑1 (MQ=255)                                                                                                             aTCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGt                                                                                                                                                   >  1:363789/1‑231 (MQ=255)                                                                                                                     ccGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTa                                                                                                                                                                                                                       >  1:677288/1‑155 (MQ=255)                                                                                                                     ccGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAAt                                                                                                                       <  1:721313/251‑1 (MQ=255)                                                                                                                     ccGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAAt                                                                                                                       <  1:327819/251‑1 (MQ=255)                                                                                                                            gTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATAtt                                                                                                                                                                                                                                              <  1:278181/125‑1 (MQ=255)                                                                                                                            gTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTAACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAg                                                                                                                >  1:257411/1‑251 (MQ=255)                                                                                                                                          gCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCTTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATaaa                                                                                                  >  1:419426/1‑251 (MQ=255)                                                                                                                                          gCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATaaa                                                                                                  >  1:785293/1‑251 (MQ=255)                                                                                                                                           cTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAg                                                                                                 <  1:869181/251‑1 (MQ=255)                                                                                                                                               gTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGaaaa                                                                                             >  1:539210/1‑251 (MQ=255)                                                                                                                                                    gTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACg                                                                                                                                                                                                                     <  1:66719/126‑1 (MQ=255)                                                                                                                                                    gTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTc                                                                                                                                                            <  1:222562/183‑1 (MQ=255)                                                                                                                                                            ccAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAAt                                                                                <  1:663837/251‑1 (MQ=255)                                                                                                                                                              aaTTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTc                                                                              <  1:852870/251‑1 (MQ=255)                                                                                                                                                               aTTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCa                                                                             >  1:78352/1‑251 (MQ=255)                                                                                                                                                                        gCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGaaaa                                                                    >  1:98658/1‑251 (MQ=255)                                                                                                                                                                           gTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGaaa                                                                                              >  1:477703/1‑222 (MQ=255)                                                                                                                                                                             gAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAg                                                                                            <  1:529415/222‑1 (MQ=255)                                                                                                                                                                             gAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGa                                                                >  1:650199/1‑250 (MQ=255)                                                                                                                                                                               atatTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCTGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCg                                                             >  1:676595/1‑251 (MQ=255)                                                                                                                                                                                     aCCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACtt                                                        <  1:533535/250‑1 (MQ=255)                                                                                                                                                                                         cACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATg                                                    <  1:570746/250‑1 (MQ=255)                                                                                                                                                                                                   tttCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGttt                                         >  1:51993/1‑251 (MQ=255)                                                                                                                                                                                                         actccaacAGATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATc                                   <  1:684268/251‑1 (MQ=255)                                                                                                                                                                                                                  gATTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCa                          >  1:115924/1‑251 (MQ=255)                                                                                                                                                                                                                   aTTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCg                                                  <  1:199987/226‑1 (MQ=255)                                                                                                                                                                                                                   aTTGCAGGCAATGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCaa                         >  1:89713/1‑251 (MQ=255)                                                                                                                                                                                                                             aTGCGCGTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCAAACTCACTGTc               <  1:678283/251‑1 (MQ=255)                                                                                                                                                                                                                                  cgTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAAc                                                                  >  1:289732/1‑195 (MQ=255)                                                                                                                                                                                                                                  cgTGTAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCAAACTCACTGTCAGGtt          >  1:667932/1‑251 (MQ=255)                                                                                                                                                                                                                                   gtgtAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCAAACTCACTGTCAGGTTg         >  1:218161/1‑251 (MQ=255)                                                                                                                                                                                                                                    tgtAATACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCAAACTCACTGTCAGGTTGa        >  1:616397/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTACAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGa                                                 >  1:699268/1‑206 (MQ=255)                                                                                                                                                                                                                                          aCAAATGTCCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCAAACTCACTGTCAGGTTGAATACCa  >  1:208670/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   AATATCGGCTTTTTTCACGCTATCGGGATCGCCGCTGATAAGCGGATGCCATGAGGGCAACGGTTTTCCTTCATGTAATAAACGATAAGCACAGGTGGCGGGTAACCAATCGAAATCCGACAAGTTTTCTTTGGTGAGCTTGGTGCAGTCTTCTTCCAATTCAAAACGCCGTGAATATTGACCGCACTTTCCCGTTTCCAACTCCAACAGATTGCAGGCAATGCGCGTGTAATACAAATGTTCATATTTGCCATAGCCGTCAATATATTTACGATAGCAACATTTCCCACACCCGTCACATAACGCCTCCCACTCGGCATCGTTCATGTCAAGTAAGGTTTTGTGTAGCCAAAAATCGGGTTCTAATTGCATAGCGAAAAATGATAAAGAAAAGTGCGGTCAAAATTCATCGTGAAAAACGACCGCACTTTATGCGATTGAGTTTAAAATCCTTCTTTCAAACTCACTGTCAGGTTGAATACCA  >  1/1298410‑1298893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑