Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,297,207	G→A	intergenic (+137627/+123730)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,297,207	0	G	A	100.0%	209.7 / NA	73	intergenic (+137627/+123730)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/42);  total (31/42)

ATCCCATTGGTTAGCTTTACGACGGGCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACGACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGTTGATAAGCA  >  1/1296971‑1297451                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     aTCCCATTGGTTAGCTTTACGACGGGCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAg                                                                                                                                                                                                                                         <  1:39149/250‑1 (MQ=255)              gCTTTACGACGGGCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTa                                                                                                                                                                                                                                      >  1:857831/1‑240 (MQ=255)               cTTTACGACGGGCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTAc                                                                                                                                                                                                                           >  1:51239/1‑250 (MQ=255)                 ttACGACGGGCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACcac                                                                                                                                                                                                                        <  1:803748/251‑1 (MQ=255)                      acgGGCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGaaa                                                                                                                                                                                                                   <  1:828389/251‑1 (MQ=255)                         ggCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAAcc                                                                                                                                                                                                                 <  1:128074/250‑1 (MQ=255)                                       gCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTgg                                                                                                                                                                                                   <  1:741174/250‑1 (MQ=255)                                            acacTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGc                                                                                                                                                                                                         <  1:786481/239‑1 (MQ=255)                                            acacTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCca                                                                                                                                                                                             >  1:545041/1‑251 (MQ=255)                                            acacTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCca                                                                                                                                                                                             >  1:730026/1‑251 (MQ=255)                                                             gATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACg                                                                                                                                                                             >  1:887048/1‑250 (MQ=255)                                                                     aCGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGac                                                                                                                                                                     <  1:260258/250‑1 (MQ=255)                                                                          gTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGt                                                                                                                                                                >  1:891359/1‑250 (MQ=255)                                                                           tATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTc                                                                                                                                                               <  1:661058/250‑1 (MQ=255)                                                                              cGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACAt                                                                                                                                                           <  1:500627/251‑1 (MQ=255)                                                                                  aCGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAAcc                                                                                                                                                                                                                 >  1:874377/1‑193 (MQ=255)                                                                                       aCTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTc                                                                                                                                                         >  1:609504/1‑244 (MQ=255)                                                                                       aCTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGaaa                                                                                                                                                  >  1:631271/1‑251 (MQ=255)                                                                                        cTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTCGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATg                                                                                                                                                     <  1:527049/247‑1 (MQ=255)                                                                                                     gCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAAt                                                                                                                                                                                                                                  <  1:476395/157‑1 (MQ=255)                                                                                                      cATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACt                                                                                                                                   <  1:491782/251‑1 (MQ=255)                                                                                                       aTAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACtt                                                                                                                                  <  1:854560/251‑1 (MQ=255)                                                                                                       aTAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACtt                                                                                                                                  <  1:199943/251‑1 (MQ=255)                                                                                                             gTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATAc                                                                                                                             <  1:777399/250‑1 (MQ=255)                                                                                                                 gCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACg                                                                                                                        >  1:524780/1‑251 (MQ=255)                                                                                                                       aaGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTaaaaa                                                                                                                  <  1:178424/251‑1 (MQ=255)                                                                                                                            gAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAg                                                                                                                                                                                                                                         <  1:649051/127‑1 (MQ=255)                                                                                                                            gAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGaca                                                                                                                                                                    <  1:349318/196‑1 (MQ=255)                                                                                                                            gAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGaa                                                                                                              >  1:79398/1‑250 (MQ=255)                                                                                                                               gTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTaaaa                                                                                                                   <  1:513653/242‑1 (MQ=255)                                                                                                                                ttGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTgaga                                                                                                                                                                      >  1:704371/1‑190 (MQ=255)                                                                                                                                         cAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATAc                                                                                                                                    <  1:578947/215‑1 (MQ=255)                                                                                                                                            tCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCa                                                                                             <  1:331772/251‑1 (MQ=255)                                                                                                                                              gATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCAtt                                                                                           >  1:225244/1‑251 (MQ=255)                                                                                                                                                   aTTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATc                                                                                       >  1:11390/1‑250 (MQ=255)                                                                                                                                                        aaTTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGACCAATTCCCCCACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATc                                                                                 <  1:324098/251‑1 (MQ=255)                                                                                                                                                        aaTTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGacac                                                                                                                                                                   >  1:459647/1‑169 (MQ=255)                                                                                                                                                        aaTTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAAt                                                                                  >  1:433666/1‑250 (MQ=255)                                                                                                                                                        aaTTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAAt                                                                                  >  1:734452/1‑250 (MQ=255)                                                                                                                                                        aaTTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATc                                                                                 <  1:27894/251‑1 (MQ=255)                                                                                                                                                          tttATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATAc                                                                                                                                    >  1:709114/1‑198 (MQ=255)                                                                                                                                                          tttATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCAt                                                                               >  1:782800/1‑251 (MQ=255)                                                                                                                                                          tttATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCAt                                                                               >  1:800982/1‑251 (MQ=255)                                                                                                                                                           ttATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCAt                                                                                                                               <  1:34746/202‑1 (MQ=255)                                                                                                                                                                   ttGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAatcat                                                                        <  1:573726/249‑1 (MQ=255)                                                                                                                                                                     gCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACACCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAatc                                                                          <  1:614493/245‑1 (MQ=255)                                                                                                                                                                                aCTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACg                                                                                                                            <  1:282790/184‑1 (MQ=255)                                                                                                                                                                                aCTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACgg                                                           <  1:23381/249‑1 (MQ=255)                                                                                                                                                                                           gAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCa                                                                                <  1:696784/217‑1 (MQ=255)                                                                                                                                                                                                   cccACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAAc                                                             <  1:553930/228‑1 (MQ=255)                                                                                                                                                                                                      acacGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGAc                                   <  1:478645/251‑1 (MQ=255)                                                                                                                                                                                                        acGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGActc                                 <  1:100314/251‑1 (MQ=255)                                                                                                                                                                                                                tGCCGGGTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCaataa                                           >  1:513867/1‑233 (MQ=255)                                                                                                                                                                                                                    gggTGTAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATa                     >  1:254003/1‑251 (MQ=255)                                                                                                                                                                                                                      gtgtAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACg                                                                                                                            <  1:457935/146‑1 (MQ=255)                                                                                                                                                                                                                      gtgtAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTc                                                   >  1:727789/1‑219 (MQ=255)                                                                                                                                                                                                                      gtgtAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATaca                   >  1:289188/1‑251 (MQ=255)                                                                                                                                                                                                                      gtgtAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATac                    <  1:121652/250‑1 (MQ=255)                                                                                                                                                                                                                      gtgtAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATac                    <  1:846271/250‑1 (MQ=255)                                                                                                                                                                                                                      gtgtAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATac                    <  1:550136/250‑1 (MQ=255)                                                                                                                                                                                                                         tAAGCGCATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACAcc                 <  1:832286/250‑1 (MQ=255)                                                                                                                                                                                                                            gcgcATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTAcccc                         >  1:609132/1‑239 (MQ=255)                                                                                                                                                                                                                              gcATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGt           >  1:325395/1‑251 (MQ=255)                                                                                                                                                                                                                              gcATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGt           >  1:292791/1‑251 (MQ=255)                                                                                                                                                                                                                               cATTGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGtt          <  1:878907/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGGATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGTTGAt       <  1:144615/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCaataa                                           <  1:625134/214‑1 (MQ=255)                                                                                                                                                                                                                                   ggATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGTTGAt       >  1:542679/1‑250 (MQ=255)                                                                                                                                                                                                                                    gATGGCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAg            <  1:73717/244‑1 (MQ=255)                                                                                                                                                                                                                                       ggCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATAc                                                                                                                                    <  1:521167/121‑1 (MQ=255)                                                                                                                                                                                                                                       ggCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCaataa                                           >  1:176849/1‑210 (MQ=255)                                                                                                                                                                                                                                       ggCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGActc                                 >  1:284745/1‑220 (MQ=255)                                                                                                                                                                                                                                       ggCGACAACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGTTGATAAGCa  >  1:816551/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     ATCCCATTGGTTAGCTTTACGACGGGCTTCCGTCATCGGCACGACACTCGGCAACGGACTGATTTGTGACGCGGTATCGGGACGTAACTGAGAACTGAAGGCATAAGGGTCGGCTTTTAAGCGGAGGTTGCCATGGCAATCGATTAATTCAAATTTATACAGTTGCCCCAAAGAGACTTTCGGCAGGAACAATTCCCACACGCCGCTTGCCGGGTGTAAGCGCATTGGATGGCGACGACCGTCCCAATAGTTAAAATCGCCTACCACAGAAACCCGTTTGGCATTTGGCGCCCACAAACGGAAATTCACGCCTGAGACACCGTCACATTCAATGAAATGCGCGCCTAATACTTCATACGGACGTAAAAACGAACCTTCCGCCAACAACCATTCATCCAAATCATTAATCATCGGGTGATAACGGTACGGGTCTTCAATAATTTGTGACTCTTTACCCCAATACACCTGTAGTTGATAAGCA  >  1/1296971‑1297451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑