Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,295,182	C→T	intergenic (+135602/+125755)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,295,182	0	C	T	100.0%	189.8 / NA	67	intergenic (+135602/+125755)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/37);  total (30/37)

AACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAACCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCCTCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAGGGCGAAGTTCGTGATC  >  1/1294958‑1295427                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      aaCCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCa                                                                                                                                                                                                                             <  1:163696/251‑1 (MQ=255) aaCCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCa                                                                                                                                                                                                                             <  1:419054/251‑1 (MQ=255)   ccGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCcaccac                                                                                                                                                                                                                                       >  1:254464/1‑239 (MQ=255)      gCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAg                                                                                                                                                                                                                         <  1:635584/250‑1 (MQ=255)       cATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAggg                                                                                                                                                                                                                       <  1:92192/251‑1 (MQ=255)       cATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAggg                                                                                                                                                                                                                       <  1:127447/251‑1 (MQ=255)       cATAGGTGCCGCGTATTGTCACTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAggg                                                                                                                                                                                                                       <  1:378653/251‑1 (MQ=255)                   gTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAAt                                                                                                                                                                                                           >  1:189214/1‑251 (MQ=255)                   gTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAAt                                                                                                                                                                                                           >  1:46183/1‑251 (MQ=255)                    tATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAAtt                                                                                                                                                                                                          <  1:571584/251‑1 (MQ=255)                        gTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAAttt                                                                                                                                                                                                         >  1:745331/1‑248 (MQ=255)                            ctGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGa                                                                                                                                                                                                  >  1:348577/1‑251 (MQ=255)                               gTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCATGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGAttt                                                                                                                                                                                               >  1:538826/1‑251 (MQ=255)                                       gTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCaa                                                                                                                                                                                       >  1:475780/1‑251 (MQ=255)                                                 tGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCa                                                                                                                                                                             <  1:95923/251‑1 (MQ=255)                                                  ggTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCa                                                                                                                                                                                                                             >  1:392229/1‑202 (MQ=255)                                                   gTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCAtt                                                                                                                                                                           >  1:538097/1‑251 (MQ=255)                                                   gTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCAtt                                                                                                                                                                           >  1:480175/1‑251 (MQ=255)                                                      aaaCCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCAttttt                                                                                                                                                                        <  1:406737/251‑1 (MQ=255)                                                         ccTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAg                                                                                                                                                                                     <  1:289371/235‑1 (MQ=255)                                                         ccTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGa                                                                                                                                                                     <  1:387658/251‑1 (MQ=255)                                                                      actcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAggg                                                                                                                                                                                                                       >  1:860870/1‑188 (MQ=255)                                                                      actcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTc                                                                                                                                                             <  1:238887/246‑1 (MQ=255)                                                                       ctcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCcaccac                                                                                                                                                                                                                                       <  1:199675/171‑1 (MQ=255)                                                                       ctcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCTGTCAACGTGCGGTCATTTTTTGATGTGATTCTGAAg                                                                                                                                                        >  1:387253/1‑250 (MQ=255)                                                                       ctcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGa                                                                                                                                                                     <  1:129196/237‑1 (MQ=255)                                                                       ctcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAg                                                                                                                                                        >  1:612408/1‑250 (MQ=255)                                                                       ctcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAg                                                                                                                                                        >  1:877716/1‑250 (MQ=255)                                                                       ctcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAg                                                                                                                                                        >  1:671561/1‑250 (MQ=255)                                                                        tcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGcc                                                                                                                                                      <  1:102674/251‑1 (MQ=255)                                                                        tcaTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGcc                                                                                                                                                      <  1:755305/251‑1 (MQ=255)                                                                          aTACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAg                                                                                                                                                                                     <  1:566407/218‑1 (MQ=255)                                                                            acacAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAGCCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTg                                                                                                                                                                                   <  1:658945/218‑1 (MQ=255)                                                                             cacaATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGc                                                                                                                                                                                                                                    <  1:418356/168‑1 (MQ=255)                                                                              acaATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTg                                                                                                                                                <  1:130832/251‑1 (MQ=255)                                                                                 aTGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAATCCCCGCTTGCGTTTCAGGCGAGCTTAAATACGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGt                                                                                                                                             <  1:38452/251‑1 (MQ=255)                                                                                 aTGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGt                                                                                                                                             <  1:684939/251‑1 (MQ=255)                                                                                         ggTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTg                                                                                                                                     >  1:88290/1‑251 (MQ=255)                                                                                          gTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTg                                                                                                                                     >  1:437651/1‑250 (MQ=255)                                                                                                ggCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCAccc                                                                                                                               <  1:770201/250‑1 (MQ=255)                                                                                                   gTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCAttttt                                                                                                                                                                        <  1:576546/206‑1 (MQ=255)                                                                                                               ttGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATg                                                                                                               >  1:21112/1‑251 (MQ=255)                                                                                                                     ctctGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCacac                                                                                                         <  1:632939/251‑1 (MQ=255)                                                                                                                     ctctGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCacac                                                                                                         <  1:80852/251‑1 (MQ=255)                                                                                                                                 aaTGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAAtt                                                                                             >  1:679399/1‑251 (MQ=255)                                                                                                                                     cACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAAcc                                                                                         <  1:374541/251‑1 (MQ=255)                                                                                                                                         aTCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGc                                                                                                                                                       <  1:322676/185‑1 (MQ=255)                                                                                                                                         aTCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGt                                                                                     >  1:25161/1‑251 (MQ=255)                                                                                                                                         aTCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGt                                                                                     >  1:861158/1‑251 (MQ=255)                                                                                                                                            aaCCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGa                                                                                  >  1:809039/1‑251 (MQ=255)                                                                                                                                                    ccGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGAt                                                                          <  1:25617/251‑1 (MQ=255)                                                                                                                                                        tttCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACGAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTcc                                                                       >  1:419699/1‑250 (MQ=255)                                                                                                                                                          tCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCacac                                                                                                         <  1:141491/214‑1 (MQ=255)                                                                                                                                                           cGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCg                                                                     >  1:8439/1‑249 (MQ=255)                                                                                                                                                            ggTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACATGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGct                                                                   >  1:712776/1‑250 (MQ=255)                                                                                                                                                             gTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGctca                                                                 >  1:808480/1‑250 (MQ=255)                                                                                                                                                                  tAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCtt                                                             >  1:105822/1‑250 (MQ=255)                                                                                                                                                                       ggCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGctc                                                                  <  1:548499/240‑1 (MQ=255)                                                                                                                                                                        gCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCAttttt                                                                                                                                                                        >  1:821765/1‑137 (MQ=255)                                                                                                                                                                                       tcgCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGTATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCt                                        <  1:422975/250‑1 (MQ=255)                                                                                                                                                                                              acaaAAACCACGCTTGCGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCTGTTgcgc                                 >  1:50260/1‑250 (MQ=255)                                                                                                                                                                                                              cGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAggg                <  1:326599/251‑1 (MQ=255)                                                                                                                                                                                                              cGTTTCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAggg                <  1:459066/251‑1 (MQ=255)                                                                                                                                                                                                                 ttCAGGCGAGCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAgggaga             <  1:589820/251‑4 (MQ=255)                                                                                                                                                                                                                          gCTTAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAGGGAGAAGTTCGTGa    >  1:122765/1‑251 (MQ=255)                                                                                                                                                                                                                            ttAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAGGGAGAAGTTCGTGATc  <  1:667133/251‑1 (MQ=255)                                                                                                                                                                                                                            ttAAATCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCATCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAGGGAGAAGTTCGTGATc  <  1:779806/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      AACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAACTCATACACAATGGTTTCGGTCCAGGGCGTATTCGGCGCTTTGTCACTCTGCCAGTCAAATGCACGATCAACCACCACCGCTTTCGGTGCATAGCGGGCGTTATCCCGCTCGTCGCTCAACAAAAACCACGCTTGCGTTTCAGGCGAGCTTAAACCCGGTTTGCCCACCACGGCCTTGGCATAAGGGTCCAGCATCAATTTCTGCGGATTTGCCGTCAAAGTGCGGTCATTTTTTGATGTGATTCTGAAGCCATATTGGGTTCCGTTTGCCACCCCGCTTAACGCCAAATGCCACACGTTGTCGGAATTAACCATGTTGAAACGGGATTCCCGCTCCTCTTCAAACAGACAAAGTTCCACCTGTTGCGCCGCCGAAGAATACAGGGCGAAGTTCGTGATC  >  1/1294958‑1295427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑