Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,294,788	C→T	intergenic (+135208/+126149)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,294,788	0	C	T	100.0%	197.4 / NA	73	intergenic (+135208/+126149)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/39);  total (34/39)

CGACGGGTAATGTGATTGGACAAATTCAGCATATTGCCGCAACCGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGCGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTATCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAAC  >  1/1294543‑1295028                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 cGACGGGTAATGTGATTGGACAAATTCAGCATATTGCCGCAACCGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAg                                                                                                                                                                                                                                               <  1:244237/249‑1 (MQ=255) cGACGGGTAATGTGATTGGACAAATTCAGCATATTGCCGCAACCGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGc                                                                                                                                                                                                                                              <  1:29393/250‑1 (MQ=255)  gACGGGTAATGTGATTGGACAAATTCAGCATATTGCCGCAACCGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCaa                                                                                                                                                                                                                                            >  1:884222/1‑251 (MQ=255)      ggTAATGTGATTGGACAAATTCAGCATATTGCCGCAACCGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAatat                                                                                                                                                                                                                                         <  1:218191/250‑1 (MQ=255)           tgtgATTGGACAAATTCAGCATATTGCCGCAACCGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGt                                                                                                                                                                                                                                   <  1:646628/251‑1 (MQ=255)                                           ccGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAAcc                                                                                                                                                                                                   <  1:726113/251‑1 (MQ=255)                                               tCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCaa                                                                                                                                                                                               <  1:492776/251‑1 (MQ=255)                                                   gTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATCGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACa                                                                                                                                                                                                                      >  1:876882/1‑224 (MQ=255)                                                   gTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCaataat                                                                                                                                                                                           >  1:385052/1‑251 (MQ=255)                                                   gTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCaataat                                                                                                                                                                                           >  1:279635/1‑251 (MQ=255)                                                     ttAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTg                                                                                                                                                                                         <  1:858197/251‑1 (MQ=255)                                                        aaTATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACgg                                                                                                                                                                                                            <  1:72488/229‑1 (MQ=255)                                                         atatTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATa                                                                                                                                                                                     >  1:758256/1‑251 (MQ=255)                                                             tCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAAccc                                                                                                                                                                                 <  1:129737/251‑1 (MQ=255)                                                             tCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACAGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAAccc                                                                                                                                                                                 <  1:118606/251‑1 (MQ=255)                                                                 cTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCtt                                                                                                                                                                             >  1:628423/1‑251 (MQ=255)                                                                  ttGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCttt                                                                                                                                                                            <  1:179254/251‑1 (MQ=255)                                                                       gggTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAAc                                                                                                                                                                                                    >  1:312468/1‑222 (MQ=255)                                                                       gggTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTttgttg                                                                                                                                                                        <  1:16502/250‑1 (MQ=255)                                                                             gCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTttgttg                                                                                                                                                                        <  1:226879/244‑1 (MQ=255)                                                                                aataatAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGt                                                                                                                                                              <  1:544510/251‑1 (MQ=255)                                                                                 ataataGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGtt                                                                                                                                                             >  1:108637/1‑251 (MQ=255)                                                                                         tttCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTac                                                                                                                                                      <  1:413659/250‑2 (MQ=255)                                                                                         tttCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTac                                                                                                                                                      <  1:268109/250‑2 (MQ=255)                                                                                                atcaATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAAt                                                                                                                                              >  1:603417/1‑251 (MQ=255)                                                                                                    aTGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTa                                                                                                                                           >  1:243425/1‑250 (MQ=255)                                                                                                              gACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGcaacaa                                                                                                                                 >  1:401907/1‑250 (MQ=255)                                                                                                              gACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGcaacaa                                                                                                                                 >  1:298114/1‑250 (MQ=255)                                                                                                                   aaGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGAc                                                                                                                           <  1:64895/251‑1 (MQ=255)                                                                                                                      gTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACggg                                                                                                                                       >  1:696148/1‑236 (MQ=255)                                                                                                                      gTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTg                                                                                                                         >  1:869430/1‑250 (MQ=255)                                                                                                                       ttGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGc                                                                                                                        <  1:325253/250‑1 (MQ=255)                                                                                                                               tATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATAc                                                                                                                <  1:847185/250‑1 (MQ=255)                                                                                                                                aTTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCt                                                                                                              <  1:119004/251‑1 (MQ=255)                                                                                                                                         aTTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGAt                                                                                                                                                                                      <  1:484486/170‑1 (MQ=255)                                                                                                                                              gccgTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTttgttg                                                                                                                                                                        <  1:805337/179‑1 (MQ=255)                                                                                                                                                    tGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTg                                                                                                                                                                  >  1:316305/1‑179 (MQ=255)                                                                                                                                                      gTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTg                                                                                                                                                                                         <  1:426746/154‑1 (MQ=255)                                                                                                                                                      gTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCctc                                                                                                                                                                               <  1:730934/164‑1 (MQ=255)                                                                                                                                                      gTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTac                                                                                                                                                      >  1:860182/1‑188 (MQ=255)                                                                                                                                                      gTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCg                                                                                        >  1:711787/1‑251 (MQ=255)                                                                                                                                                      gTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCg                                                                                        >  1:835070/1‑251 (MQ=255)                                                                                                                                                      gTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCg                                                                                        >  1:99674/1‑251 (MQ=255)                                                                                                                                                      gTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCg                                                                                        >  1:148950/1‑251 (MQ=255)                                                                                                                                                           acacCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTg                                                                                    >  1:806983/1‑250 (MQ=255)                                                                                                                                                                  gTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCttt                                                                                                                                                                            <  1:791816/155‑1 (MQ=255)                                                                                                                                                                             cTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCttt                                                                                                                                                                            <  1:514338/144‑1 (MQ=255)                                                                                                                                                                                 aaTGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAgg                                                             <  1:3399/251‑1 (MQ=255)                                                                                                                                                                                  aTGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGt                                                            <  1:617477/251‑1 (MQ=255)                                                                                                                                                                                    gcctgcctCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTttg                                                                                                                                                                           >  1:868117/1‑138 (MQ=255)                                                                                                                                                                                        gcctCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCcgcg                                                      >  1:392058/1‑251 (MQ=255)                                                                                                                                                                                         cctCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGt                                                     <  1:846888/251‑1 (MQ=255)                                                                                                                                                                                                 aaGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGtctc                                             <  1:901130/251‑1 (MQ=255)                                                                                                                                                                                                   ggCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGtct                                              >  1:558837/1‑248 (MQ=255)                                                                                                                                                                                                     cTTTCACCATTTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGt                                         <  1:548844/251‑1 (MQ=255)                                                                                                                                                                                                       ttCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTg                                                                                                                                                                                         <  1:406271/105‑1 (MQ=255)                                                                                                                                                                                                       ttCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTaa                                       >  1:872496/1‑251 (MQ=255)                                                                                                                                                                                                         cACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAAt                                      >  1:49402/1‑250 (MQ=255)                                                                                                                                                                                                          aCCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTc                                    <  1:736312/251‑1 (MQ=255)                                                                                                                                                                                                               gtgtTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGt                                                            >  1:457519/1‑222 (MQ=255)                                                                                                                                                                                                               gtgtTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGttt                               >  1:893922/1‑251 (MQ=255)                                                                                                                                                                                                                 gtTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTc                                    <  1:893675/244‑1 (MQ=255)                                                                                                                                                                                                                   ttAAATTCATTTAACGGGTTCGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTaa                           <  1:223951/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGCCATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCacac       <  1:518179/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAAcc                                                                                                                                                                                                   >  1:232000/1‑61 (MQ=255)                                                                                                                                                                                                                                         ccATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCttt                                                                                                                                                                            <  1:659794/84‑1 (MQ=255)                                                                                                                                                                                                                                         ccATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATAc                                                                                               <  1:610729/161‑1 (MQ=255)                                                                                                                                                                                                                                         ccATATTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACact      >  1:683639/1‑250 (MQ=255)                                                                                                                                                                                                                                           atatTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTttgttg                                                                                                                                                                        >  1:103556/1‑86 (MQ=255)                                                                                                                                                                                                                                           atatTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACactcaa   >  1:37852/1‑251 (MQ=255)                                                                                                                                                                                                                                           atatTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACactcaa   >  1:715199/1‑251 (MQ=255)                                                                                                                                                                                                                                            tatTGGCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACactcaac  <  1:774650/251‑1 (MQ=255)                                                                                                                                                                                                                                                ggCTTGTGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTACCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTAt                                                   >  1:715696/1‑198 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 CGACGGGTAATGTGATTGGACAAATTCAGCATATTGCCGCAACCGGTCCAGTTTAAATATTCGTCTTGCGGGGTTTGCCAATAATAGGTTTCGTCATCAATGCCACGTTGACTAAAGGTTGGGAAGTATTTTTCCGATTCCGCCGTATGGTTGAACACCACGTCTAAAATCACTTCAATGCCTGCCTCATGCAAGGCTTTCACCATGTGTTTAAATTCATTTAACGGGTTCGCCATATTGGCTTGCGAGCAATATTTCGGTTCCACGGCAAACATGGCTAACGGGTTGTAACCCCAATAATTGGATAACCCTCTTTGTTGTAAGTGCGGTTCGCTTATCGCGTAATTTACGGGCAACAACTCGACTGCCGTTATACCTAACTTTTTCAAATACGCAATCGTTTGCGCATGTGCCAAACCGGCATAGGTGCCGCGTATTGTCTCTGGTAATTCCGTTTGTAATTTGGTAAAACCTTTCACACTCAAC  >  1/1294543‑1295028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑