Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,294,239	G→T	intergenic (+134659/+126698)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,294,239	0	G	T	100.0%	166.0 / NA	61	intergenic (+134659/+126698)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/37);  total (24/37)

TGATTTTCTACCGCACTTTGGTGCGGATCGGTTAAATCCAATTCGCTGCCTTCAAAGCCATGGTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCGGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGTTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGGTGTTTCGCGCCCTAACGCAGTCGCCA  >  1/1293994‑1294477                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               tGATTTTCTACCGCACTTTGGTGCGGATCGGTTAAATCCAATTCGCTGCCTTCAAAGCCATGGTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCaaa                                                                                                                                                                                                                                           <  1:83181/251‑1 (MQ=255)  gATTTTCTACCGCACTTTGGTGCGGATCGGTTAAATCCAATTCGCTGCCTTCAAAGCCATGGTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAg                                                                                                                                                                                                                                          >  1:415135/1‑251 (MQ=255)                                    aTCCAATTCGCTGCCTTCAAAGCCATGGTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTGTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCAc                                                                                                                                                                                                                          <  1:443796/233‑1 (MQ=255)                                         aTTCGCTGCCTTCAAAGCCATGGTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGcac                                                                                                                                                                                                   <  1:386600/251‑1 (MQ=255)                                              cTGCCTTCAAAGCCATGGTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATatc                                                                                                                                                                                              <  1:576331/251‑1 (MQ=255)                                                              ggTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCAc                                                                                                                                                                                                                          >  1:460464/1‑207 (MQ=255)                                                              ggTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCg                                                                                                                                                                              >  1:314297/1‑251 (MQ=255)                                                               gTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACt                                                                                                                                                                                                                         <  1:273637/207‑1 (MQ=255)                                                               gTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACata                                                                                                                                                                                                <  1:277596/232‑1 (MQ=255)                                                                   taACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCa                                                                                                                                                                         <  1:76601/251‑1 (MQ=255)                                                                      cTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAGATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTc                                                                                                                                                                      <  1:833825/251‑1 (MQ=255)                                                                                cATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAaataa                                                                                                                                                            <  1:134969/251‑1 (MQ=255)                                                                                                    tcttcGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTg                                                                                                                                        <  1:753307/251‑1 (MQ=255)                                                                                                                 cTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATg                                                                                                                            <  1:412767/250‑1 (MQ=255)                                                                                                                 cTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATg                                                                                                                            <  1:826195/250‑1 (MQ=255)                                                                                                                   tCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGc                                                                                                                                   <  1:623580/241‑1 (MQ=255)                                                                                                                           tttttGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTcc                                                                                                                  <  1:670794/250‑1 (MQ=255)                                                                                                                                ggTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCt                                                                                                                                             <  1:839146/218‑1 (MQ=255)                                                                                                                                 gTTATAACTGGTTAAATCGCGCAAGGTGGAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCTCATATCATCACGAAAACGGTCGTGCCGTTCAGCGATATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAggc                                                                                                            <  1:895538/250‑2 (MQ=255)                                                                                                                                           gTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCAGATGTCCCAAGGCTCACTGATTa                                                                                                  <  1:96358/250‑1 (MQ=255)                                                                                                                                              ggtggaactttgtctgtttgaagatGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAAttt                                                                                              >  1:739343/24‑251 (MQ=255)                                                                                                                                              aaaTCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAAttt                                                                                              <  1:750770/251‑1 (MQ=255)                                                                                                                                               aaTCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAggc                                                                                                            >  1:107904/1‑235 (MQ=255)                                                                                                                                                    cgcAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCAccc                                                                                         <  1:621564/250‑1 (MQ=255)                                                                                                                                                         ggTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGt                                                                                                                           <  1:521493/211‑1 (MQ=255)                                                                                                                                                          gTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCaa                                                                                  >  1:827324/1‑251 (MQ=255)                                                                                                                                                               gCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCg                                                                             >  1:779886/1‑251 (MQ=255)                                                                                                                                                                ccATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTc                                                                              <  1:120492/249‑1 (MQ=255)                                                                                                                                                                  atcatGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGt                                                                                                                                                                                >  1:116071/1‑149 (MQ=255)                                                                                                                                                                       gAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTg                                                                      >  1:72469/1‑250 (MQ=255)                                                                                                                                                                         gCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAAttt                                                                                              <  1:599129/224‑1 (MQ=255)                                                                                                                                                                          cGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTgcgc                                                                   <  1:510488/250‑1 (MQ=255)                                                                                                                                                                            gTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTc                                                                                                                                                                      >  1:759077/1‑149 (MQ=255)                                                                                                                                                                            gTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAaataa                                                                                                                                                            <  1:835771/159‑1 (MQ=255)                                                                                                                                                                            gTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGa                                                                                                                       <  1:26785/196‑1 (MQ=255)                                                                                                                                                                               ataaAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCAc                                                                                                                                                                                                                                  >  1:532951/1‑86 (MQ=255)                                                                                                                                                                               ataaAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAAttt                                                                                              <  1:703285/218‑1 (MQ=255)                                                                                                                                                                                    aTTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATatc                                                                                                                                                                                              <  1:790012/117‑1 (MQ=255)                                                                                                                                                                                      tCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAaac                                                      <  1:64803/251‑1 (MQ=255)                                                                                                                                                                                      tCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAaac                                                      <  1:707261/251‑1 (MQ=255)                                                                                                                                                                                      tCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAaac                                                      <  1:362985/251‑1 (MQ=255)                                                                                                                                                                                      tCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAaac                                                      <  1:732718/251‑1 (MQ=255)                                                                                                                                                                                           gACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAAcg                                                  >  1:763091/1‑250 (MQ=255)                                                                                                                                                                                            aCGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAAttt                                                                                              >  1:361390/1‑205 (MQ=255)                                                                                                                                                                                            aCGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAAcgc                                                 >  1:356201/1‑250 (MQ=255)                                                                                                                                                                                              gTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATGATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAAcgccg                                               >  1:448801/1‑250 (MQ=255)                                                                                                                                                                                              gTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCaa                                                                                                               <  1:324880/186‑1 (MQ=255)                                                                                                                                                                                              gTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAaac                                                      >  1:720895/1‑243 (MQ=255)                                                                                                                                                                                                aTGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCAt                                            >  1:815077/1‑251 (MQ=255)                                                                                                                                                                                                   cGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATgg                                          <  1:531094/250‑1 (MQ=255)                                                                                                                                                                                                   cGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATgg                                          <  1:199615/250‑1 (MQ=255)                                                                                                                                                                                                         gcgcCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGt                                                              >  1:581055/1‑224 (MQ=255)                                                                                                                                                                                                         gcgcCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCAt                                            >  1:211472/1‑242 (MQ=255)                                                                                                                                                                                                                tCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCgg                            <  1:359801/251‑1 (MQ=255)                                                                                                                                                                                                                  cTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGgtg                          >  1:342820/1‑251 (MQ=255)                                                                                                                                                                                                                    gTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCGCTGATTGATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGGTGtt                        >  1:437395/1‑251 (MQ=255)                                                                                                                                                                                                                    gTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAAttt                                                                                              >  1:272266/1‑181 (MQ=255)                                                                                                                                                                                                                    gTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCAACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGGTGtt                        >  1:730267/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGGTGTTTCGCGCCCTAACGc          <  1:247343/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccGGAGCCCGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGGTGTTTCGCGCCCTAACGc          <  1:520057/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGCCAGACGTTCTGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGCTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGGTGTTTCGCGCCCTAACGCAGTCGCCa  >  1:792495/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               TGATTTTCTACCGCACTTTGGTGCGGATCGGTTAAATCCAATTCGCTGCCTTCAAAGCCATGGTTATAACTGTAATTTTCATTGCGCCCGTCACGGTTTTCTTCGCCATTGGCTTCATTGTGTTTTTGGTTATAACTGGTTAAATCGCGCAAGGTGAAGCCATCATGAGCGGTAATAAAATTCAGCGACGTATGCGGCAGGCGCCGTTCCTGTTTAAAAATGTCGCCGGAGCCCGCCAGACGTTCGGCAAAGGCGCCCACTTCCCCACTTTTCCACAACCAGAAACGGCACATATCATCACGAAAACGGTCGTTCCATTCAGCGAAATAATGGGGAAAATTGCCTAATTGATAGCCATAATGTCCGATGTCCCAAGGTTCACTGATTAATTTCACCCGCTGCAAACTCGACGTTTGCGCAATGTCGTTAAACAACGCCGCATGGTGATTAAAATCCGGTGTTTCGCGCCCTAACGCAGTCGCCA  >  1/1293994‑1294477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑