Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,291,448	A→G	intergenic (+131868/+129489)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,291,448	0	A	G	100.0%	212.7 / NA	77	intergenic (+131868/+129489)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/41);  total (36/41)

GTTTAAGATACCCACCAAGCGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACAGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTGGCATCGTGATAAGGATTACCTTCGCGTGCGTACAAA  >  1/1291213‑1291693                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      gTTTAAGATACCCACCAAGCGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCa                                                                                                                                                                                                                                        >  1:378242/1‑251 (MQ=255)         tACCCACCAAGCGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGa                                                                                                                                                                                                                                <  1:402137/251‑1 (MQ=255)             caccaAGCGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATgg                                                                                                                                                                                                                            >  1:786884/1‑251 (MQ=255)                 aaGCGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGaa                                                                                                                                                                                                                        >  1:672292/1‑251 (MQ=255)                    cGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAacac                                                                                                                                                                                                                     <  1:655773/251‑1 (MQ=255)                    cGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAacac                                                                                                                                                                                                                     <  1:828732/251‑1 (MQ=255)                    cGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAacac                                                                                                                                                                                                                     <  1:786708/251‑1 (MQ=255)                     gACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGcc                                                                                                                                                                                                                                         >  1:693902/1‑230 (MQ=255)                      aCCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGAt                                                                                                                                                                                                                                              <  1:469754/224‑1 (MQ=255)                      aCCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCg                                                                                                                                                                                                                   <  1:8644/251‑1 (MQ=255)                        ccTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGAc                                                                                                                                                                                                                 <  1:888410/251‑1 (MQ=255)                                   agagTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCg                                                                                                                                                                                                      >  1:107526/1‑251 (MQ=255)                                            aaTAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATg                                                                                                                                                                                             <  1:528862/251‑1 (MQ=255)                                             aTAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGa                                                                                                                                                                                            <  1:634556/251‑1 (MQ=255)                                                 aCCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGAtata                                                                                                                                                                                        >  1:749510/1‑251 (MQ=255)                                                  ccTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATgg                                                                                                                                                                                                                            >  1:493138/1‑214 (MQ=255)                                                  ccTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATaa                                                                                                                                                                                       >  1:51505/1‑251 (MQ=255)                                                     tGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATa                                                                                                                                                                                    <  1:653931/251‑1 (MQ=255)                                                        aaaCCGTACGAAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATAcgc                                                                                                                                                                                 <  1:107071/251‑1 (MQ=255)                                                                    aaTTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCGTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATcc                                                                                                                                                                     <  1:120819/251‑1 (MQ=255)                                                                         cGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGt                                                                                                                                                                <  1:710740/251‑1 (MQ=255)                                                                           gCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGa                                                                                                                                                                                            <  1:473497/221‑1 (MQ=255)                                                                                 tAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCg                                                                                                                                                        <  1:468045/251‑1 (MQ=255)                                                                                     ttctttcGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTggg                                                                                                                                                    <  1:335535/251‑1 (MQ=255)                                                                                      tctttcGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGc                                                                                                                                                   <  1:772797/251‑1 (MQ=255)                                                                                       ctttcGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCa                                                                                                                                                  >  1:175758/1‑251 (MQ=255)                                                                                           cGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATgg                                                                                                                                                                                                                            <  1:279846/173‑1 (MQ=255)                                                                                                 ggTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAacac                                                                                                                                                                                                                     >  1:67237/1‑174 (MQ=255)                                                                                                 ggTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGa                                                                                                                                                                                            >  1:200257/1‑199 (MQ=255)                                                                                                 ggTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATa                                                                                                                                                                                    >  1:598872/1‑207 (MQ=255)                                                                                                 ggTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATa                                                                                                                                                                                    >  1:557074/1‑207 (MQ=255)                                                                                                   tCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTc                                                                                                                                       <  1:150637/250‑1 (MQ=255)                                                                                                      ggCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGCGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTgcg                                                                                                                                   >  1:97977/1‑251 (MQ=255)                                                                                                        cTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTgcgc                                                                                                                                  <  1:75801/250‑1 (MQ=255)                                                                                                              gccgTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGcac                                                                                                                                             <  1:279311/233‑1 (MQ=255)                                                                                                              gccgTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGccacca                                                                                                                           >  1:641685/1‑251 (MQ=255)                                                                                                                   gACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATAc                                                                                                                                                                                   >  1:261905/1‑190 (MQ=255)                                                                                                                        caTCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATATGcc                                                                                                                                                                                                                                         >  1:325338/1‑131 (MQ=255)                                                                                                                        caTCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCaaa                                                                                                                 >  1:847736/1‑251 (MQ=255)                                                                                                                          tCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAAcc                                                                                                               <  1:756930/251‑1 (MQ=255)                                                                                                                             gAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGa                                                                                                                                                                                            <  1:558621/171‑1 (MQ=255)                                                                                                                             gAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGc                                                                                                                         >  1:611257/1‑238 (MQ=255)                                                                                                                             gAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGAt                                                                                                            >  1:71262/1‑251 (MQ=255)                                                                                                                             gAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGAt                                                                                                            >  1:37018/1‑251 (MQ=255)                                                                                                                               ataataTAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAAt                                                                                                                                                                                                                              >  1:693196/1‑135 (MQ=255)                                                                                                                                 aataTAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGcc                                                                                                        <  1:327043/251‑1 (MQ=255)                                                                                                                                  atatAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGc                                                                                                                                                                                                            >  1:890657/1‑150 (MQ=255)                                                                                                                                  atatAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATAcgc                                                                                                                                                                                 <  1:314486/177‑1 (MQ=255)                                                                                                                                  atatAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAAcc                                                                                                               >  1:458622/1‑243 (MQ=255)                                                                                                                                    ataAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTc                                                                                                                                       <  1:322100/217‑1 (MQ=255)                                                                                                                                            gCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGa                                                                                                                                                                                            >  1:738529/1‑156 (MQ=255)                                                                                                                                            gCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTgcg                                                                                                                                   <  1:615951/213‑1 (MQ=255)                                                                                                                                            gCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCAc                                                                                              <  1:777639/250‑1 (MQ=255)                                                                                                                                            gCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCAc                                                                                              <  1:538749/250‑1 (MQ=255)                                                                                                                                                     tAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATa                                                                                                                                                                                    >  1:668249/1‑155 (MQ=255)                                                                                                                                                     tAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGacccaac                                                                                     >  1:597904/1‑250 (MQ=255)                                                                                                                                                      aaGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGacccaaccc                                                                                   <  1:616851/251‑1 (MQ=255)                                                                                                                                                         aaaTTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACccaacccaac                                                                                <  1:235198/251‑1 (MQ=255)                                                                                                                                                           aTTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAg                                                                              <  1:109214/251‑1 (MQ=255)                                                                                                                                                              tGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAggg                                                                            >  1:857195/1‑250 (MQ=255)                                                                                                                                                              tGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGc                                                                           <  1:36080/251‑1 (MQ=255)                                                                                                                                                                    aagaaTTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATg                                                                                                                                                                                             >  1:898862/1‑131 (MQ=255)                                                                                                                                                                    aagaaTTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCg                                                                     <  1:78035/251‑1 (MQ=255)                                                                                                                                                                        aTTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGatat                                                                                                                                                                                         >  1:602607/1‑131 (MQ=255)                                                                                                                                                                        aTTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTta                                                                 >  1:738076/1‑251 (MQ=255)                                                                                                                                                                                      acaTGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGaa                                                                                                                                                                                                                               <  1:591258/79‑1 (MQ=255)                                                                                                                                                                                           gAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGaa                                                                                                                                                                                                                               <  1:359085/74‑1 (MQ=255)                                                                                                                                                                                           gAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCAc                                                                                              >  1:323008/1‑203 (MQ=255)                                                                                                                                                                                                  tCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAAtt                                       <  1:41331/251‑1 (MQ=255)                                                                                                                                                                                                   cTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTg                                      <  1:192200/251‑1 (MQ=255)                                                                                                                                                                                                        ggCAAACCGATTTCAAATAAATGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCAACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGCTTATAATTGTCGCCGTAATCGTTGTAATTGGCAt                                  >  1:190920/1‑250 (MQ=255)                                                                                                                                                                                                                        ataaatGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTGGCATCGTGATAAGGATTACCt                 <  1:199117/251‑1 (MQ=255)                                                                                                                                                                                                                             tGGCGATAATCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTGGCATCGTGATAAGGATTACCTTcgcg            >  1:78112/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTGGCATCGTGa                             <  1:392863/226‑1 (MQ=255)                                                                                                                                                                                                                                      tCAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCC‑GTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTGGCATCGTGATAAGGATTACCTTCGCGTGCGTACaaa  >  1:54779/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGacc                                                                                         >  1:580421/1‑164 (MQ=255)                                                                                                                                                                                                                                       cAAACGGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTGGCATCGTGATAAGGATTACCTTCGCGTGCGTACaaa  <  1:514944/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      GTTTAAGATACCCACCAAGCGACCCTCTTGATCGAGAGTGGACAATAAACCTTGTAAACCGTACGCAAATTCCGGCGTGGTAATTTCTTTCGCATAGGTCGGGCTCACCGCCGTGACCACATCGGAATAATATAAACCGGCTTTCAAATAAGAAATTTGTCCGAAGAATTCCAAACCGTTCACATGGAACATTTCTGCCGGCAAACCGATTTCAAATAAATGGCGATAATCAAACAGACCTTGATACGCCAGATTATGAATGGTGAACACCGACTTGGCAGGGCGACCTTTATGATATAAATACGCGGCGGTCAATCCGGCGTGCCAGTCGTGGGCATGCACCACTTCTGCGCGCCACCAGCTGTCCAAACCGATCGCCAGTTCTGCACCGACCCAACCCAACAGGGCGAAGCGTTTATAATTGTCGCCGTAATCGTTGTAATTGGCATCGTGATAAGGATTACCTTCGCGTGCGTACAAA  >  1/1291213‑1291693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑