Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,290,333	C→T	intergenic (+130753/+130604)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,290,333	0	C	T	100.0%	85.6 / NA	34	intergenic (+130753/+130604)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

GCAATCATGGCGTTAGACAAAGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCACATTGATTTCAGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTCAGTTAACAATCAAAAAAACAGGTGAAATTTTAATCATTCGAGATAAAAAAACAATAATAATCTTCCGTTCAAGCCGTATTTTTACTTGAACGAAAGATTAACTATTTCATTAC  >  1/1290101‑1290514                                                                                                                                                                                                                                         |                                                                                                                                                                                      gCAATCATGGCGTTAGACAAAGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGgatgat                                                                                                                                                                      <  1:569628/250‑1 (MQ=255)        tGGCGTTAGACAAAGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAg                                                                                                                                                              <  1:781956/251‑1 (MQ=255)          gCGTTAGACAAAGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTgctgtcccttatacacatccccga                                                                                                                                                            >  1:114841/1‑228 (MQ=255)          gCGTTAGACAAAGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGaa                                                                                                                                                            >  1:681722/1‑251 (MQ=255)                gACAAAGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGgaga                                                                                                                                                      >  1:807821/1‑251 (MQ=255)                    aaGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCGATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCg                                                                                                                                                  >  1:415167/1‑251 (MQ=255)                       tGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAg                                                                                                                                               <  1:64851/251‑1 (MQ=255)                             cGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATcc                                                                                                                                         <  1:668959/251‑1 (MQ=255)                               aTCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCAt                                                                                                                                       >  1:204757/1‑251 (MQ=255)                               aTCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAATTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCAt                                                                                                                                       >  1:438661/1‑251 (MQ=255)                                 cAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGCTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTg                                                                                                                                                                                   >  1:630779/1‑205 (MQ=255)                                            tGGATAAATAATAAACGCGACGTGACCCTTCCGTTCTGGCTCGGCGGGCTGTGTTTATCCACCCTTCCTTCACAAAATCACGCACTGCATATAAGGTCGCTTTTAACCAATCGCGCTGGCTTGCCTCTTTAGTCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGGTGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAAt                                                                                                                          <  1:472159/251‑1 (MQ=255)                                            tGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAAt                                                                                                                          <  1:357487/251‑1 (MQ=255)                                            tGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAAt                                                                                                                          <  1:125171/251‑1 (MQ=255)                                              gATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAAt                                                                                                                          <  1:669582/249‑1 (MQ=255)                                                           cgcgACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAg                                                                                                                        >  1:680971/1‑238 (MQ=255)                                                               aCGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAA‑GTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCAt                                                                                                                                       >  1:554912/1‑218 (MQ=255)                                                                            gTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTGACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGgag                                                                                                                                                       <  1:409659/190‑1 (MQ=255)                                                                            gTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGAATGATCGTACAAGaa                                                                                                                                                            >  1:431765/1‑185 (MQ=255)                                                                                                    aTCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTCAGTTAACAATCAAAAAAA‑AGGTGAAATTTTAATCATTCGAGATaaaaa                                                                  <  1:527694/250‑1 (MQ=255)                                                                                                    aTCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTCAGTTAACAATCAAAAAAA‑AGGTGAAATTTTAATCATTCGAGATaaaaa                                                                  <  1:70081/250‑1 (MQ=255)                                                                                                           cTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTcc                                                                                                                     >  1:113283/1‑193 (MQ=255)                                                                                                                                cTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCAt                                                                                                                                       >  1:714525/1‑154 (MQ=255)                                                                                                                                  gCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAAt                                                                                                                          >  1:325913/1‑165 (MQ=255)                                                                                                                                  gCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAg                                                                                                                        <  1:821467/167‑1 (MQ=255)                                                                                                                                  gCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAg                                                                                                                        <  1:58027/167‑1 (MQ=255)                                                                                                                                  gCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTc                                                                                                                   >  1:319200/1‑172 (MQ=255)                                                                                                                                               cgTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATaa                                                                                                                             <  1:187389/149‑1 (MQ=255)                                                                                                                                                                 gACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTcc                                                                                                                     >  1:188223/1‑139 (MQ=255)                                                                                                                                                                   cTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTCAGTTAACAATCAAAAAAA‑AGGTGAAATTTTAATCATTCGAGATAAAAAAACAATAATAATCTTCCGTTCAAGCCGTATTTTTACTTGAACGAAAGATTAACTATTTCATTAc  <  1:430481/251‑1 (MQ=255)                                                                                                                                                                          ctTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTCAGTTAACAAt                                                                                                         <  1:717596/142‑1 (MQ=255)                                                                                                                                                                          ctTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTCAGTTAACAATCAAAAAAA‑AGGTGAAATTTTAATCATTCGAGATAAAAAAACAATAATAATCTTCCGTTCAAGCCGTATTTTTACTTGAACGAAAGATTAACTATTTCATTAc  >  1:30756/1‑244 (MQ=255)                                                                                                                                                                                             aaGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGgag                                                                                                                                                       >  1:315983/1‑77 (MQ=255)                                                                                                                                                                                                                           cttcAATGATGCCATATTGATTTCGGGATGATCGTACAAGAATGGAGAATCGAAg                                                                                                                                               >  1:483239/1‑55 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                      GCAATCATGGCGTTAGACAAAGTGCGACCGATCAAAAACTCCATGGATAAATAATAAACGCGACGTGAACCTTCCGTTCTGGCTCGGCGGGCTGTGGTGATCCAACCTTCCGTCACAAAATCACGCACTGCATATAAGGTCGCGTTTAACCAATCGCGCTGACTTGCCTCTTTAGGCGAGCGCCCGATAAGAAAAATCAGTTTATAGACGATCGCCTTCTTCAATGATGCCACATTGATTTCAGGATGATCGTACAAGAATGGAGAATCGAAGTTATCCATTATCATATAAAATAGTCCTCAGTTAACAATCAAAAAAACAGGTGAAATTTTAATCATTCGAGATAAAAAAACAATAATAATCTTCCGTTCAAGCCGTATTTTTACTTGAACGAAAGATTAACTATTTCATTAC  >  1/1290101‑1290514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑