Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,289,021	G→A	intergenic (+129441/+131916)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,289,021	0	G	A	100.0%	145.9 / NA	55	intergenic (+129441/+131916)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/29);  total (26/29)

TGGCAAGACGCACTTTGTTATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGGCAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCAAAAATCATTTGTAAATGACGTGGCAGGATTTTCGCCATCATTTCTAC  >  1/1288788‑1289262                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  tGGCAAGACGCACTTTGTTATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTAcc                                                                                                                                                                                                                                  <  1:69234/251‑1 (MQ=255)    cAAGACGCACTTTGTTATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCAt                                                                                                                                                                                                                                <  1:279930/250‑1 (MQ=255)        aCGCACTTTGTTATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAAt                                                                                                                                                                                                                           <  1:83525/251‑1 (MQ=255)             cTTTGTTATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAg                                                                                                                                                                                                                      >  1:495615/1‑251 (MQ=255)                  ttATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAAt                                                                                                                                                                                                                 <  1:694320/251‑1 (MQ=255)                   tATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAAtt                                                                                                                                                                                                                <  1:328700/251‑1 (MQ=255)                        aaTTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTgccg                                                                                                                                                                                                            <  1:365528/250‑1 (MQ=255)                         aTTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTgccgcc                                                                                                                                                                                                          <  1:223239/251‑1 (MQ=255)                             aaTGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCacac                                                                                                                                                                                                      <  1:283872/251‑1 (MQ=255)                             aaTGTCGGCAATCGCACGTTTAAATTCAGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCacac                                                                                                                                                                                                      <  1:449178/251‑1 (MQ=255)                                             cGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTGACCAACGACGTGGAGCAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAgg                                                                                                                                                                                                                                       >  1:739235/1‑202 (MQ=255)                                                  aaaTTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTgag                                                                                                                                                                                       <  1:322763/245‑1 (MQ=255)                                                            tCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACaa                                                                                                                                                                       >  1:89416/1‑251 (MQ=255)                                                                       ttCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTAc                                                                                                                                                                             <  1:773303/234‑1 (MQ=255)                                                                           ttCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCAc                                                                                                                                                        <  1:726033/251‑1 (MQ=255)                                                                                   tgcaatTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGtt                                                                                                                                                <  1:606408/251‑1 (MQ=255)                                                                                            gCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACct                                                                                                                                        <  1:334461/250‑1 (MQ=255)                                                                                                        aCGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAg                                                                                                                           <  1:60980/251‑1 (MQ=255)                                                                                                         cGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACaa                                                                                                                                                                                                                                                <  1:177198/133‑1 (MQ=255)                                                                                                         cGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGa                                                                                                                          <  1:120110/251‑1 (MQ=255)                                                                                                                    gCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCAc                                                                                                                                                        >  1:285711/1‑210 (MQ=255)                                                                                                                    gCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGa                                                                                                               >  1:136786/1‑251 (MQ=255)                                                                                                                       gATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGa                                                                                                            >  1:480323/1‑251 (MQ=255)                                                                                                                        atatatTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCacac                                                                                                                                                                                                      >  1:773060/1‑160 (MQ=255)                                                                                                                           tatTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATc                                                                                                        <  1:608067/251‑1 (MQ=255)                                                                                                                            atTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATc                                                                                                        >  1:678250/1‑250 (MQ=255)                                                                                                                               gATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTG‑ATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCatat                                                                                                   >  1:464134/1‑251 (MQ=255)                                                                                                                                aTCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGAc                                                                                                                                                                                               >  1:687492/1‑159 (MQ=255)                                                                                                                                 tCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGtt                                                                                                                                                >  1:488496/1‑205 (MQ=255)                                                                                                                                   aaaaaGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCgg                                                                                                <  1:169368/251‑1 (MQ=255)                                                                                                                                              ccAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAg                                                                                     <  1:427220/251‑1 (MQ=255)                                                                                                                                                  tttCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGttt                                                                                 >  1:643704/1‑251 (MQ=255)                                                                                                                                                                   cTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAg                                                                <  1:563914/251‑1 (MQ=255)                                                                                                                                                                     aaCCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGt                                                                                               <  1:632528/218‑1 (MQ=255)                                                                                                                                                                     aaCCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTAGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTg                                                              >  1:443001/1‑251 (MQ=255)                                                                                                                                                                      aCCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGAc                                                                                                                                                                         >  1:810158/1‑143 (MQ=255)                                                                                                                                                                       ccAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGAt                                                            >  1:652415/1‑251 (MQ=255)                                                                                                                                                                             acgTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTcac                                                                             <  1:870729/228‑1 (MQ=255)                                                                                                                                                                             acgTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTaa                                                      >  1:685445/1‑251 (MQ=255)                                                                                                                                                                                 ggAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGt                                                                                            >  1:740569/1‑209 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAAcc                                                                                                                                                                     >  1:718373/1‑135 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACg                                                                                                                                                  <  1:685833/154‑1 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCAcc                                                                                                                                         >  1:615874/1‑163 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATg                                                                                        >  1:86019/1‑212 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGa                                                                  <  1:178977/234‑1 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCa                                                 >  1:363725/1‑251 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCa                                                 >  1:515447/1‑251 (MQ=255)                                                                                                                                                                                  gAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCa                                                 >  1:790923/1‑251 (MQ=255)                                                                                                                                                                                                    cGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCacac                                                                                                                                                                                                      >  1:227669/1‑84 (MQ=255)                                                                                                                                                                                                      ttAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAgg                                                                   <  1:678399/213‑1 (MQ=255)                                                                                                                                                                                                      ttAGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCAAAAATCATTTGTAAATGACg                             >  1:328744/1‑251 (MQ=255)                                                                                                                                                                                                        aGTGAATCGTTCCGGATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCAAAAATCATTTGTAAATGACGTg                           >  1:234776/1‑251 (MQ=255)                                                                                                                                                                                                                     ggATAAATACGGGCAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGt                                                                                                                                                 <  1:321688/120‑1 (MQ=255)                                                                                                                                                                                                                                  cAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCAAAAATCATTTGTAAATGACGTGGCAGGATTTTCGCCATCATTTCTAc  <  1:649484/250‑1 (MQ=255)                                                                                                                                                                                                                                  cAAAATCGACAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCAAAAATCATTTGTAAATGACGTGGCAGGATTTTCGCCATCATTTCTAc  <  1:369448/250‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  TGGCAAGACGCACTTTGTTATCGAATTTAATGTCGGCAATCGCACGTTTAAATTCGGCTTCATTGGCGTGTTCTTTCAGCAATGCAATTTGGCTTAAATCGCAACGCCAATCGCTGCCGATATATTGATCAAAAAGCGATGCCAATTTCGGGTTCGCTACGGCTAACCAACGACGTGGAGTAATACCGTTGGTGACGTTAGTGAATCGTTCCGGATAAATACGGGCAAAATCGGCAAAGGTTGAGGTTACCATTAAATCGGAGTGAATTGCCGCCACACCGTTGACTTTGTGAGAACCGACTACGGACAACCAGCCCATGCGCACTTTACGTTGGTCACCTTCTTCCACCAAAGATACGCGACGGATGAAATCCATATCGGTAGTGATGTAGGTTTTCACATATTCGAGGAAGTGATCGTTAATTTCAAAAATCATTTGTAAATGACGTGGCAGGATTTTCGCCATCATTTCTAC  >  1/1288788‑1289262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑