Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,287,913	G→A	intergenic (+128333/+133024)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,287,913	0	G	A	100.0%	192.7 / NA	71	intergenic (+128333/+133024)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/41);  total (30/41)

GTGCCAGTAATCTGGATTGGTTGCACGAACAAATAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTGATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGATAATAGTCGTGATATTGGAGACCCTGTAAC  >  1/1287670‑1288144                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        gTGCCAGTAATCTGGATTGGTTGCACGAACAAATAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCg                                                                                                                                                                                                                                  >  1:74581/1‑251 (MQ=255)    ccAGTAATCTGGATTGGTTGCACGAACAAATAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGatt                                                                                                                                                                                                                               >  1:732802/1‑251 (MQ=255)       gTAATCTGGATTGGTTGCACGAACAAATAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGattatt                                                                                                                                                                                                                            >  1:680453/1‑251 (MQ=255)         aaTCTGGATTGGTTGCACGAACAAATAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTAtttt                                                                                                                                                                                                                          >  1:76426/1‑251 (MQ=255)         aaTCTGGATTGGTTGCACGAACAAATAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTAtttt                                                                                                                                                                                                                          >  1:468032/1‑251 (MQ=255)                               aaaTAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAatat                                                                                                                                                                                                    <  1:761446/251‑1 (MQ=255)                               aaaTAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAatat                                                                                                                                                                                                    <  1:397986/251‑1 (MQ=255)                               aaaTAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAatat                                                                                                                                                                                                    <  1:284205/251‑1 (MQ=255)                                      aaaaGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGat                                                                                                                                                                                             <  1:900540/251‑1 (MQ=255)                                                   gTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCatat                                                                                                                                                                                >  1:667065/1‑251 (MQ=255)                                                       tttATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTcc                                                                                                                                                                                                 >  1:52199/1‑230 (MQ=255)                                                       tttATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTc                                                                                                                                                                              >  1:379122/1‑249 (MQ=255)                                                         tataTTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTa                                                                                                                                                                          <  1:356972/251‑1 (MQ=255)                                                          atatTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTcc                                                                                                                                                                                                 <  1:117142/227‑1 (MQ=255)                                                           tatTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAAt                                                                                                                                                                        <  1:612886/251‑1 (MQ=255)                                                           tatTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAAt                                                                                                                                                                        <  1:275692/251‑1 (MQ=255)                                                           tatTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAAt                                                                                                                                                                        <  1:41387/251‑1 (MQ=255)                                                            atTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATc                                                                                                                                                                       <  1:29129/251‑1 (MQ=255)                                                             ttCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCg                                                                                                                                                                      <  1:224080/251‑1 (MQ=255)                                                                        gTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAAt                                                                                                                                                                        >  1:452786/1‑238 (MQ=255)                                                                               tCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGaaa                                                                                                                                                     <  1:835146/250‑1 (MQ=255)                                                                                 atatTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAg                                                                                                                                                  <  1:381750/251‑1 (MQ=255)                                                                                   atTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGcc                                                                                                                                                <  1:743450/251‑1 (MQ=255)                                                                                          gCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTg                                                                                                                                         >  1:326026/1‑251 (MQ=255)                                                                                               gAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAAt                                                                                                                                                                        >  1:767085/1‑215 (MQ=255)                                                                                               gAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGaaa                                                                                                                                                     <  1:78189/234‑1 (MQ=255)                                                                                                    ggTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAAt                                                                                                                                                    >  1:389219/1‑230 (MQ=255)                                                                                                    ggTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGAt                                                                                                                                    >  1:794927/1‑246 (MQ=255)                                                                                                    ggTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGtttt                                                                                                                               >  1:489032/1‑251 (MQ=255)                                                                                                    ggTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGtttt                                                                                                                               >  1:272814/1‑251 (MQ=255)                                                                                                     gTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCgg                                                                                                                                                                                                            <  1:479231/173‑1 (MQ=255)                                                                                                     gTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTg                                                                                                                              >  1:110626/1‑251 (MQ=255)                                                                                                     gTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTg                                                                                                                              >  1:728144/1‑251 (MQ=255)                                                                                                        taAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAAt                                                                                                                           <  1:724147/251‑1 (MQ=255)                                                                                                        taAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAAt                                                                                                                           <  1:553893/251‑1 (MQ=255)                                                                                                                   aaacaaaacCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGcc                                                                                                                                                >  1:599083/1‑219 (MQ=255)                                                                                                                   aaacaaaacCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCaa                                                                                                                >  1:593306/1‑251 (MQ=255)                                                                                                                   aaacaaaacCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCAT‑AATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAAt                                                                                                               >  1:670301/1‑251 (MQ=255)                                                                                                                     acaaaacCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAAt                                                                                                                           >  1:256778/1‑238 (MQ=255)                                                                                                                      caaaacCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATcc                                                                                                             <  1:750052/251‑1 (MQ=255)                                                                                                                                   cTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAAt                                                                                                                                                                        <  1:666730/179‑1 (MQ=255)                                                                                                                                              gtcgtcTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTtc                                                                                      <  1:384699/250‑1 (MQ=255)                                                                                                                                              gtcgtcTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTtc                                                                                      <  1:279613/250‑1 (MQ=255)                                                                                                                                                                    cTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCatat                                                                >  1:523238/1‑250 (MQ=255)                                                                                                                                                                    cTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATc                                                               <  1:435020/251‑1 (MQ=255)                                                                                                                                                                            acacTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAAc                                                       <  1:762254/251‑1 (MQ=255)                                                                                                                                                                              acTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTg                                                     <  1:721819/251‑1 (MQ=255)                                                                                                                                                                                    aaTTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATgg                                                                                                                         <  1:863274/177‑1 (MQ=255)                                                                                                                                                                                     aTTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAAt                                              >  1:176769/1‑251 (MQ=255)                                                                                                                                                                                                   tttCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATa                                                         <  1:468482/226‑1 (MQ=255)                                                                                                                                                                                                    ttCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCGCTTTTTGCATATCCGCATAACTGCGGAAATCGGCGAATGCCTGat                               <  1:425286/251‑1 (MQ=255)                                                                                                                                                                                                       ataaataaACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGataat                            <  1:683405/251‑1 (MQ=255)                                                                                                                                                                                                       ataaataaACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGataat                            <  1:212534/251‑1 (MQ=255)                                                                                                                                                                                                       ataaataaACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCACATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGataat                            <  1:91690/251‑1 (MQ=255)                                                                                                                                                                                                        taaataaaCAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCa                                                                   <  1:235413/211‑1 (MQ=255)                                                                                                                                                                                                        taaataaaCAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAAc                                                       >  1:473906/1‑223 (MQ=255)                                                                                                                                                                                                        taaataaaCAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAAc                                                       >  1:835485/1‑223 (MQ=255)                                                                                                                                                                                                            taaaCAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATaa                                                        >  1:774224/1‑218 (MQ=255)                                                                                                                                                                                                            taaaCAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGATAATAGTCg                       <  1:263955/251‑1 (MQ=255)                                                                                                                                                                                                             aaaCAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCgg                                                  >  1:387533/1‑223 (MQ=255)                                                                                                                                                                                                               aCAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGataa                             >  1:737622/1‑242 (MQ=255)                                                                                                                                                                                                                     ccccGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGATAATAGTCGTGATGTTgg              <  1:676647/251‑1 (MQ=255)                                                                                                                                                                                                                       ccGCTCAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGATAATAGTCGTGATATTGgag            <  1:454014/251‑1 (MQ=255)                                                                                                                                                                                                                            cAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTc                                                                                                                  >  1:303050/1‑144 (MQ=255)                                                                                                                                                                                                                            cAAATATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGATAATAGt                         <  1:868172/233‑1 (MQ=255)                                                                                                                                                                                                                              aaTATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCgg                                                                                                                                                                                                            <  1:521213/52‑1 (MQ=255)                                                                                                                                                                                                                              aaTATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCa                                                                                                        <  1:713082/152‑1 (MQ=255)                                                                                                                                                                                                                              aaTATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAAc                                                       <  1:519518/201‑1 (MQ=255)                                                                                                                                                                                                                              aaTATGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGataa                             >  1:793297/1‑227 (MQ=255)                                                                                                                                                                                                                                 atGAACGGGGTTGATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGATAATAGTCGTGATATTGGAGACCCTGTAAc  <  1:557317/251‑1 (MQ=255)                                                                                                                                                                                                                                             gATTTTTAATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCat                                                                  <  1:32333/175‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                        GTGCCAGTAATCTGGATTGGTTGCACGAACAAATAAAAAAAGGACAGTCGGTGATTTATATTCTGGTTGCCGTTTTAGTCATATTCGTTGCCTGGAAATGGTATAAAGCACGCAAAACAAAACCCGCCAACTAAAAGTGCGGTCGTCTTTGATGCACTTTTCACTTCAAAAACACTGAAAATTTCAACCGCACTTTTCATAAATAAACAAAACCCCGCTCAAATATGAACGGGGTTGATTTTTGATGACCGATTATTTTGCCGGTTTTAGCGGTTCAATATTCCAGATATTTTCCGCATATTCCTTAATCGTCCGGTCCGAGGAGAAATAGCCCATGTTGACGATGTTTTGAATGGTGCTTTCAATCCACGCATCTCGATTTTTATACTTCTCGTCCACCACTTTTTGCATATCCACATAACTGCGGAAATCGGCGAATGCCTGATAATAGTCGTGATATTGGAGACCCTGTAAC  >  1/1287670‑1288144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑